BD PuraMatrix peptide hydrogel as a culture system for human fetal Schwann cells in spinal cord regeneration

Moradi, Fatemeh; Bahktiari, Mehrdad; Joghataei, Mohammad Taghi; Nobakht, Maliheh; Soleimani, Masoud; Hasanzadeh, Gholamreza; Fallah, Ali; Zarbakhsh, Sam; Hejazian, Leila Beigom; Shirmohammadi, Maryam; Maleki, Fatemeh

doi:10.1002/jnr.23120

Cited by 66 publications

(51 citation statements)

References 36 publications

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“…RADA also showed spinal cord regeneration efficiency despite the delay in treatment [107]. Moradi et al transplanted RADA nanofiber scaffold with Schwann cells to spinal cord 7 d after injury.…”

Section: Rada/cells For Cns Treatmentmentioning

confidence: 97%

Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

Liu

Wang

et al. 2014

Front. Mater. Sci.

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Central nervous system (CNS) presents a complex regeneration problem due to the inability of central neurons to regenerate correct axonal and dendritic connections. However, recent advances in developmental neurobiology, cell signaling, cell-matrix interaction, and biomaterials technologies have forced a reconsideration of CNS regeneration potentials from the viewpoint of tissue engineering and regenerative medicine. The applications of a novel tissue regeneration-inducing biomaterial and stem cells are thought to be critical for the mission. The use of peptide nanofiber hydrogels in cell therapy and tissue engineering offers promising perspectives for CNS regeneration. Self-assembling peptide undergo a rapid transformation from liquid to gel upon addition of counterions or pH adjustment, directly integrating with the host tissue. The peptide nanofiber hydrogels have mechanical properties that closely match the native central nervous extracellular matrix, which could enhance axonal growth. Such materials can provide an optimal three dimensional microenvironment for encapsulated cells. These materials can also be tailored with bioactive motifs to modulate the wound environment and enhance regeneration. This review intends to detail the recent status of selfassembling peptide nanofiber hydrogels for CNS regeneration.

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Section: Rada/cells For Cns Treatmentmentioning

confidence: 97%

Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

Liu

Wang

et al. 2014

Front. Mater. Sci.

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“…PuraMatrix (BD), a self-assembling peptide hydrogel, is a three-dimensional cell culture system for various types of cells and has been applied to regenerative tissue repair. Transplantation of SCs cultured in PuraMatrix promotes their survival in the injured spinal cord and reduces astrogliosis and locomotor impairment (Moradi et al, 2012). Alginic acid sodium hydrogel co-transplantation with SCs reduces their apoptosis when they are placed within the injured spinal cord and enhances SC-mediated recovery of locomotor function after SCI .…”

Section: Combination Of Sc Transplantation With Novel Molecules/matermentioning

confidence: 99%

“…Previous studies suggest that the use of matrices to suspend SCs for transplantation can improve their longterm survival within the injured spinal cord (Patel et al, 2010;Moradi et al, 2012;Wang et al, 2012). Matrigel (BD), a matrix composed of laminin, collagen type IV, heparan sulfate proteoglycans, entactin, and growth factors, has been employed with SC grafting in various models of SCI.…”

Section: Combination Of Sc Transplantation With Novel Molecules/matermentioning

confidence: 99%

Schwann cell transplantation for spinal cord injury repair: its significant therapeutic potential and prospectus

Kanno¹,

Pearse²,

Ozawa

et al. 2015

Reviews in the Neurosciences

105

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AbstractTransplantation of Schwann cells (SCs) is a promising therapeutic strategy for spinal cord repair. The introduction of SCs into the injured spinal cord has been shown to reduce tissue loss, promote axonal regeneration, and facilitate myelination of axons for improved sensorimotor function. The pathology of spinal cord injury (SCI) comprises multiple processes characterized by extensive cell death, development of a milieu inhibitory to growth, and glial scar formation, which together limits axonal regeneration. Many studies have suggested that significant functional recovery following SCI will not be possible with a single therapeutic strategy. The use of additional approaches with SC transplantation may be needed for successful axonal regeneration and sufficient functional recovery after SCI. An example of such a combination strategy with SC transplantation has been the complementary administration of neuroprotective agents/growth factors, which improves the effect of SCs after SCI. Suspension of SCs in bioactive matrices can also enhance transplanted SC survival and increase their capacity for supporting axonal regeneration in the injured spinal cord. Inhibition of glial scar formation produces a more permissive interface between the SC transplant and host spinal cord for axonal growth. Co-transplantation of SCs and other types of cells such as olfactory ensheathing cells, bone marrow mesenchymal stromal cells, and neural stem cells can be a more effective therapy than transplantation of SCs alone following SCI. This article reviews some of the evidence supporting the combination of SC transplantation with additional strategies for SCI repair and presents a prospectus for achieving better outcomes for persons with SCI.

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“…21 Spinal cord samples were incubated in the 4% PFA at 4°C for 24 hours and then embedded in paraffin (Merck, Darmstadt, Germany). 21 Spinal cord samples were incubated in the 4% PFA at 4°C for 24 hours and then embedded in paraffin (Merck, Darmstadt, Germany).…”

Section: Histological Stainingmentioning

confidence: 99%

Targeting axonal degeneration and demyelination using combination administration of 17β‐estradiol and Schwann cells in the rat model of spinal cord injury

Namjoo

Mortezaee

Joghataei

et al. 2018

J of Cellular Biochemistry

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Schwann cells (SCs) are known to be responsible for axonal ensheathing and myelination, and their transplantation is used commonly for treatment of spinal cord injury (SCI). 17β-estradiol (E2) has also reported for its protective roles in neurons in the transplanted SCs to the SCI model. In the current study, we evaluated the roles of E2 administration before SCs transplantation in targeting SCI-induced axonal degeneration and demyelination. E2 (25 µg/kg, IP) was administered to the male Wistar rats underwent contusive SCI at T10 segment. At 7 days after injury, 1 × 10 SCs were transplanted to the injury epicenter of the spinal cord. The groups were laminectomy, SCI, SCI+E2, and SCI+E2+SCs. Functional recovery was evaluated using the Basso-Bresnahan-Beattie locomotor test. Sections from spinal cord were also assessed for histoloical staining, including Luxol fast blue, Bielschowsky's silver and immunofluorescence evaluation of myelin basic protein (MBP). The SCI group showed impaired locomotion in the hind limb, increased number of cavities within spinal cord, low observable numbers of regenerating fibers, and a significant decrease in the rate of expression for MBP. These changes were counteracted in the treatment groups ( P < 0.05 vs SCI) with no significant changes among them. From the results, it may be concluded that application of E2 and SCs could be effective when axons undergo demyelination and degenerative processes, and their combination could partly provide cumulative outcomes.

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BD PuraMatrix peptide hydrogel as a culture system for human fetal Schwann cells in spinal cord regeneration

Cited by 66 publications

References 36 publications

Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

Schwann cell transplantation for spinal cord injury repair: its significant therapeutic potential and prospectus

Targeting axonal degeneration and demyelination using combination administration of 17β‐estradiol and Schwann cells in the rat model of spinal cord injury

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