BCSG1 siRNA delivered by lentiviral vector suppressed proliferation and migration of MDA-MB-231 cells

Abstract

Breast cancer-specific gene 1 (BCSG1), also referred to as γ-synuclein (SNCG), is highly expressed in human infiltrating breast carcinomas, but not in normal or benign breast tissue. The present study aimed to evaluate the effects of BCSG1 siRNA delivered by lentiviral vector on breast cancer cells and investigate the underlying mechanisms. BCSG1 RNAi lentiviral vector was constructed and transfected into MDA-MB-231 cells. BCSG1 mRNA levels were determined by quantitative polymerase chain reaction analysis. Cell proliferation, migration and apoptosis were evaluated by using the cell counting kit‑8, Transwell assay and flow cytometry, respectively, followed by western blotting to determine the relative levels of AKT, extracellular signal‑regulated kinase (ERK), p-AKT and p-ERK expression. BCSG1 mRNA levels were significantly reduced in MDA-MB‑231 cells following transfection of BCSG1 siRNA delivered by lentiviral vector. Cell migration and proliferation were significantly decreased and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were significantly lower in the BCSG1 siRNA-treated groups compared with the control and negative control groups. Therefore, BCSG1 siRNA delivered by lentiviral vector was able to significantly reduce BCSG1 expression, suppress cell migration and proliferation, possibly through the reduction of the protein levels of p-AKT and p-ERK.

Curcumin induces re‑expression of BRCA1 and suppression of γ synuclein by modulating DNA promoter methylation in breast cancer cell lines

“…This result is consistent with a previous study that demonstrated that curcumin is a potent growth inhibitor of various breast cancer cell lines (41). Previous studies have found that BRCA1 deficiency and the aberrant expression of SNCG enhance the proliferation of cancer cells (42)(43)(44) and that the restoration of normal expression patterns to these two genes reduces cell proliferation. In the present study, the re-expression of BRCA1 in HCC-38 and UACC-3199 cells and the suppression of SNCG in T47D may have been one mechanism by which curcumin influenced the inhibition of cell proliferation in the three cell lines.…”