BCSG1 Methylation Status and BCSG1 Expression in Breast Tissues Derived from Chinese Women with Breast Cancer

Abstract

Objective: To investigate the role of DNA methylation in the regulation of BCSG1 gene expression in breast tissues derived from Chinese women with breast cancer. Methods: The methylation status of exon 1 of the BCSG1 gene in breast cancer, and matched non-neoplastic adjacent and benign lesion tissues was extensively examined using methylation-specific PCR analysis. Corresponding mRNA expression levels were determined by real-time PCR. Results: We found: (1) the BCSG1 gene was universally demethylated in all breast tissues regardless of the tissue state, although 50–60% of the samples displayed methylated products as well; (2) DNA methylation correlated to the expression of the BCSG1 gene in tumor tissues, but not in non-neoplastic adjacent and benign tissues; (3) breast tumor tissues expressed lower BCSG1 than non-neoplastic adjacent tissues. Conclusions: Our data revealed a unique expression pattern and methylation status of the BCSG1 gene in breast tissues derived from Chinese patients and suggested both methylation status and mechanisms underlying BCSG1 regulation might be closely related to the racial background.

Changes in DNA 5-Hydroxymethylcytosine Levels and the Underlying Mechanism in Non-functioning Pituitary Adenomas

“…Additionally, there are TET2 mutations in breast cancer (24). Breast cancer samples with TET2 mutations showed lower 5hmC levels than those of samples without TET2 mutations (24). However, we did not find a TET2 mutation in 6 NFPA sequences of all coding regions.…”

“…TET2 mutations are common in myeloid diseases and are directly related with 5hmC levels (14,15,22,23). Additionally, there are TET2 mutations in breast cancer (24). Breast cancer samples with TET2 mutations showed lower 5hmC levels than those of samples without TET2 mutations (24).…”

Deregulation of 2 Potassium Channels in Pancreas Adenocarcinomas

“…29 Methylation of K + channels in pancreatic cancer has never been previously described, and studies comparing methylation promoter region and protein expression are limited. 30,31 Gene promoter methylation could be associated with poor prognosis of the tumor and poor overall survival of patients. 32,33 The correlation between K V 1.3 expression and metastatic tumors and the observed trend linking methylation of K V 1.3 gene promoter to metastatic tumors prompted an investigation of methylation versus survival.…”

Physical Activity and Differential Methylation of Breast Cancer Genes Assayed from Saliva: A Preliminary Investigation

“…A considerable body of work to date has focused on DNA methylation at CpG sites in genes associated with breast cancer [6, 13–16]. While the evidence for DNA methylation effects on breast cancer development and prognosis are increasingly clear, the next step is to understand the behavioral and environmental stimuli that may mitigate or exacerbate the epigenetic modifications that increase the risk for breast cancer.…”