Bclaf1 critically regulates the type I interferon response and is degraded by alphaherpesvirus US3

Qin, Chao; Zhang, Rui; Lang, Yue; Shao, Anwen; Xu, Aotian; Feng, Wen-hai; Han, Jun; Wang, Mengdong; He, Wanwei; Yu, Cuilian; Tang, Jun

doi:10.1101/392555

Cited by 1 publication

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References 56 publications

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“…PRV WT (Bartha K61), the recombinant PRV EP0-Knockout virus (PRV-EP0 KO) and KOS strain of HSV-1 were described previously (30,31).…”

Section: Cell Culture and Virusesmentioning

confidence: 99%

Swine Promyelocytic Leukemia Isoform II Inhibits Pseudorabies Virus Infection by Suppressing Viral Gene Transcription in Promyelocytic Leukemia Nuclear Bodies

Lang

et al. 2020

J Virol

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Promyelocytic leukaemia nuclear bodies (PML-NBs) possess an important intrinsic antiviral activity against α-herpesvirus infection. PML is the structural backbone of NBs, comprising different isoforms. However, the contribution of each isoform to α-herpesvirus restriction is not well understood. Here, we report the role of PML-NBs and swine PML (sPML) isoforms in pseudorabies virus (PRV) infection in its natural host swine cells. We found that sPML-NBs exhibit an anti-PRV activity in the context of increasing the expression level of endogenous sPML. Of four sPML isoforms cloned and examined, only isoform sPML-II/IIa, not sPML-I and IVa, expressed in a sPML knockout cells inhibits PRV infection. Both the unique 7b region of sPML-II and sumoylation-dependent normal formation of PML-NBs are required. 7b possesses a transcriptional repression activity and suppresses viral gene transcription during PRV infection with the cysteine residue 589 and 599 being critically involved. We conclude that sPML-NBs inhibit PRV infection partly by repressing viral gene transcription through the 7b region of sPML-II. IMPORTANCE PML-NBs are nuclear sites that mediate the antiviral restriction of α-herpesvirus gene expression and replication. However, the contribution of each PML isoform to this activity of PML-NBs is not well characterized. Using PRV and its natural host swine cells as a system, we have discovered that the unique C-terminus of sPML isoform II is required for PML-NBs to inhibit PRV infection by directly engaging in repression of viral gene transcription. Our study not only confirms in swine cells that PML-NBs have an anti-viral function, but also presents a mechanism to suggest that PML-NBs inhibit viral infection in an isoform specific manner.

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“…PRV WT (Bartha K61), the recombinant PRV EP0-Knockout virus (PRV-EP0 KO) and KOS strain of HSV-1 were described previously (30,31).…”

Section: Cell Culture and Virusesmentioning

confidence: 99%

Swine Promyelocytic Leukemia Isoform II Inhibits Pseudorabies Virus Infection by Suppressing Viral Gene Transcription in Promyelocytic Leukemia Nuclear Bodies

Lang

et al. 2020

J Virol

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Bclaf1 critically regulates the type I interferon response and is degraded by alphaherpesvirus US3

Abstract: 13Type I interferon response plays a prominent role against viral infection, which is frequently 14 disrupted by viruses. Here, we report Bcl-2 associated transcription factor 1 (Bclaf1) is 15

Cited by 1 publication

References 56 publications

Swine Promyelocytic Leukemia Isoform II Inhibits Pseudorabies Virus Infection by Suppressing Viral Gene Transcription in Promyelocytic Leukemia Nuclear Bodies

Swine Promyelocytic Leukemia Isoform II Inhibits Pseudorabies Virus Infection by Suppressing Viral Gene Transcription in Promyelocytic Leukemia Nuclear Bodies

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