“…The ChIPs were done essentially as previously described (Williams et al, 2016), with the following modifications. 4 × 10 7 formaldehyde-fixed cells were lysed in SDS lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, supplemented with Complete, EDTA-free Protease Inhibitor Cocktail (Roche)) and diluted 4-fold with dilution buffer (16.4 mM Tris-HCl, pH 8.0, 167 mM NaCl, 1.2 mM EDTA, 0.01% SDS, 1.1% Triton X-100, supplemented with inhibitors).…”