Bcl-2, p53, and Ki-67 expression in pterygium and normal conjunctiva and their relationship with pterygium recurrence

Turan, Meydan; Turan, Gülay

doi:10.1177/1120672120945903

Cited by 13 publications

(5 citation statements)

References 26 publications

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“…While less studied than DNA damage, altered expression patterns of the apoptotic regulators p53, Bcl-2 and Bax have been shown in pterygium [ 79 , 80 ]. To emphasize the role of programmed cell death as cellular reaction, we take p53 by way of an example.…”

Section: Uv-induced Cellular Reactionsmentioning

confidence: 99%

Pterygium—The Good, the Bad, and the Ugly

Acker

Bogerd

Haagdorens

et al. 2021

Cells

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Pterygium is a multifaceted pathology that displays apparent conflicting characteristics: benign (e.g., self-limiting and superficial), bad (e.g., proliferative and potentially recurrent) and ugly (e.g., signs of preneoplastic transformation). The natural successive question is: why are we lacking reports showing that pterygium lesions become life-threatening through metastasis, especially since pterygium has considerable similarities with UV-related malignancies on the molecular level? In this review, we consider how our pathophysiological understanding of the benign pterygium pathology overlaps with ocular surface squamous neoplasia and skin cancer. The three UV-related disorders share the same initial insult (i.e., UV radiation) and responsive repair mechanisms to the ensuing (in)direct DNA damage. Their downstream apoptotic regulators and other cellular adaptations are remarkably alike. However, a complicating factor in understanding the fine line between the self-limiting nature of pterygium and the malignant transformation in other UV-related diseases is the prominent ambiguity in the pathological evaluation of pterygium biopsies. Features of preneoplastic transformation (i.e., dysplasia) are used to define normal cellular reactions (i.e., atypia and metaplasia) and vice versa. A uniform grading system could help in unraveling the true nature of this ancient disease and potentially help in identifying the earliest intervention point possible regarding the cellular switch that drives a cell’s fate towards cancer.

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Section: Uv-induced Cellular Reactionsmentioning

confidence: 99%

Pterygium—The Good, the Bad, and the Ugly

Acker

Bogerd

Haagdorens

et al. 2021

Cells

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“…The accumulation of the extracellular matrix, rather than its degradation in patients with POAG and PEG, according to scientists, occurs due to elevated levels of TIMP-2 over MMP-2. In addition, some authors noted that the pathological remodelling of the lattice plate of the sclera under the influence of MMP in patients with glaucoma, there is a loss of neural tissue, rather than its scarring [37,38]. Apparently, this fact can explain the development of glaucomatous excavation of the optic disc in glaucoma with normal intraocular pressure.…”

Section: Research Resultsmentioning

confidence: 99%

Cell-molecular mechanisms of progression of ophthalmological pathology on the background of influence of environmental factors. Literature review

Nedtzvetskaya¹,

Bagmut²,

Soboleva³

et al. 2021

SR:MS

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Modern scientists are increasingly paying attention to the molecular mechanisms of diseases of the visual organ in conditions of anthropogenic pollution. Environmental pollution is mainly due to atmospheric emissions from the metallurgical, automotive, aviation and petrochemical industries, waste from livestock farms and due to the use of mineral fertilizers and pesticides. Ukraine ranks one of the first in Europe in terms of the amount of industrial dirt per capita. The aim of this literature review was to analyze the role of extra- and intracellular protein structures and molecular mechanisms of some pathological processes of the visual organ that occur under the influence of anthropogenic stress on the human body. Material and methods. Scientific publications in foreign and Ukranian journals on relevant topics in the last 5 years, the Internet resources. Research results and their discussion. The literature review expanded the scientific understanding of the role of reparative enzyme (MGMT), vascular endothelial growth factor, Bcl-2 family proteins, p53 and Ki 67 proteins, matrix metalloproteinases in some ophthalmic pathology. Anthropoecological environmental factors have been shown to cause oxidative stress due to mitochondrial dysfunction and apoptosis, which are a component of a complex pathophysiological process in the most common diseases of the visual analyzer. Conclusions. The study of molecular mechanisms of occurrence and progression of diseases of the visual organ with the participation of protein factors makes it possible to expand the understanding of the pathogenetic links of their development in order to predict the course of the pathological process, adequate treatment and prevention

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“…The study by Tan et al [29] found that the development of pterygium was due to the disruption of the normal process of conjunctival cell apoptosis. And the expression of the anti-apoptotic protein Bcl-2 may be associated with pterygium recurrence, and it also plays a vital role in the anti-apoptotic process of pterygium cells [30] . Significant expression of Bcl-2 was observed in the basal epithelial layer of all pterygium epithelial cells, while normal conjunctiva did not show high expression of Bcl-2.…”

Section: Nintedanib Induces Apoptosis Of Pterygium Epitheliummentioning

confidence: 99%

Nintedanib induces apoptosis in human pterygium cells through the FGFR2-ERK signalling pathway

Gong¹,

Qian²,

Li³

et al. 2023

Int J Ophthalmol

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AIM: To investigate whether nintedanib can inhibit pterygium cells through the fibroblast growth factor receptor 2 (FGFR2)/extracellular-signal-regulated kinase (ERK) pathway. METHODS: Human primary pterygium cells were cultured in vitro. After treatment with nintedanib, the cell morphology was observed under microscopy, the morphological changes of the nucleus were observed after DAPI staining, apoptosis was analyzed by Annexin-V FITC/PI double staining, and the changes of apoptosis-associated proteins were detected by Western blot. The binding ability of nintedanib to FGFR2 was predicted by molecular docking. Finally, by silencing FGFR2, we explored whether nintedanib inhibited FGFR2/ERK pathway. RESULTS: The results showed that nintedanib inhibited the growth of pterygium cells and caused nuclear pyknosis. The results of Annexin-VFITC/PI double staining showed that nintedanib was able to induce early and late apoptosis of pterygium cells, significantly increasing the expression of apoptosis-associated proteins Bax and cleaved-Caspase3 (P<0.05), and reducing the expression of Bcl-2 (P<0.05). In addition, nintedanib significantly inhibited ERK1/2 phosphorylation through FGFR2 (P<0.05). After silencing the expression of FGFR2, there was no significant difference in the inhibition of ERK1/2 phosphorylation by nintedanib (P>0.05). CONCLUSION: Nintedanib induces apoptosis of pterygium cells by inhibiting FGFR2/ERK pathway.

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Bcl-2, p53, and Ki-67 expression in pterygium and normal conjunctiva and their relationship with pterygium recurrence

Cited by 13 publications

References 26 publications

Pterygium—The Good, the Bad, and the Ugly

Pterygium—The Good, the Bad, and the Ugly

Cell-molecular mechanisms of progression of ophthalmological pathology on the background of influence of environmental factors. Literature review

Nintedanib induces apoptosis in human pterygium cells through the FGFR2-ERK signalling pathway

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