Bcl-2 and Bcl-xL differentially protect human prostate cancer cells from induction of apoptosis by melanoma differentiation associated gene-7, mda-7/IL-24

Enhanced delivery of mda-7/IL-24 using a serotype chimeric adenovirus (Ad.5/3) improves therapeutic efficacy in low CAR prostate cancer cells

“…25 The P69 cells are normal human prostate epithelial cells immortalized by the SV40 T/t antigen and are cultured as described previously. 25 Cell viability was determined by standard MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays.…”

“…25 The P69 cells are normal human prostate epithelial cells immortalized by the SV40 T/t antigen and are cultured as described previously. 25 Cell viability was determined by standard MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. 25 Construction of Ad.5/3-mda-7 The genome of Ad.5/3-mda-7 ( Figure 1a) was generated by homologous DNA recombination in Escherichia coli between linearized plasmid pShCMV.mda-7 and BstBIdigested genomic DNA of Ad.5/3-Luc1 vector.…”

“…25 Cell viability was determined by standard MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. 25 Construction of Ad.5/3-mda-7 The genome of Ad.5/3-mda-7 ( Figure 1a) was generated by homologous DNA recombination in Escherichia coli between linearized plasmid pShCMV.mda-7 and BstBIdigested genomic DNA of Ad.5/3-Luc1 vector. The resultant pAd.5/3-mda-7 plasmid was selected within.…”

The antitumor activity of TRAIL and IL-24 with replicating oncolytic adenovirus in colorectal cancer

“…Each group was comprised of eight animals and the tumor growth was monitored and measured weekly and tumor volume (V) was calculated using the formula V (mm 3 ) ¼ length  width 2 /2. When the tumors were 100-150 mm 3 in size, mice were randomized into five groups and a daily dose of 5  10 8 PFU of examined viruses suspended in 100 ml of PBS or 100 ml PBS alone was administrated intratumorally for 4 days. At the end of the experiments, the tumors were harvested for histopathological and TdTmediated dUTP-biotin nick end labeling (TUNEL) analysis.…”

“…Earlier studies showed that expression of IL-24 by plasmid or replication-defective adenovirus that harbored IL-24 suppressed cell growth and induced apoptosis in a wide variety of cancer cell lines, including melanoma, breast, lung, cervix, colon and prostate carcinomas, but not normal cells. [2][3][4][5] However, the mechanism by which IL-24 induces its selective apoptosis is complicated, depending on the cell type studied. Previous studies have demonstrated that IL-24 can induce apoptosis at multiple levels by activating caspase cascade and upregulating P53, Bax, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and growth arrest and DNA damage protein family genes.…”