BBD-driven optimization of an RP-HPLC method for simultaneous analysis of two major ısoflavone aglycones in Tofu
Florentinus Dika Octa RISWANTO,
Dina Christin Ayuning PUTRI,
Michael Raharja GANI
Abstract:Tofu, one of soy food products widely consumed in Indonesia, has been reported as a source of natural protein. It is important to evaluate the contents of genistein and daidzein to provide information according to the nutrient information on soy food products. A reversed-phase high performance liquid chromatography (RP-HPLC) was developed in this study in order to analyze genistein and daidzein content in tofu samples simultaneously. Employing a Box-Behnken design (BBD) for designing the experiment, the respon… Show more
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