Bazi Bushen mitigates epigenetic aging and extends healthspan in naturally aging mice

Mao, Xinjing; Hou, Yunlong; Fang, Chao; Ma, Kai-Kuang; Zhang, Shixiong; Guo, Zhifang; Kang, Ning; Niu, Kunxu; Shen, Xiaogang; Li, Yawen; Jiang, Yuning; Song, Ye‐Qiong; Wang, Lu; Li, Hongrong; Chang, Liping; Wu, Yiling; Li, Mengnan

doi:10.1016/j.biopha.2023.114384

Cited by 13 publications

(17 citation statements)

References 56 publications

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“…Several anti-aging treatments are suggested to have an underlying epigenetic mechanism. Indeed, a reduction of epigenetic age was observed in several murine tissues after different intervention typologies, including rapamycin, dasatinib + quercitin, caloric restriction and exercise [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Human LAV-BPIFB4 Gene Therapy on the Epigenetic Clock and Health of Aged Mice

Giuliani

Barbi

Bigossi

et al. 2023

IJMS

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The homozygous genotype of the Longevity-Associated Variant (LAV) in Bactericidal/Permeability-Increasing Fold-Containing Family B member 4 (BPIFB4) is enriched in long-living individuals of three independent populations and its genetic transfer in C57BL/6J mice showed a delay in frailty progression and improvement of several biomarkers of aging and multiple aspects of health. The C57BL/6J strain is a suitable model for studying therapies aimed at extending healthy aging and longevity due to its relatively short lifespan and the availability of aging biomarkers. Epigenetic clocks based on DNA methylation profiles are reliable molecular biomarkers of aging, while frailty measurement tools are used to evaluate overall health during aging. In this study, we show that the systemic gene transfer of LAV-BPIFB4 in aged C57BL/6J mice was associated with a significant reduction in the epigenetic clock-based biological age, as measured by a three CpG clock method. Furthermore, LAV-BPIFB4 gene transfer resulted in an improvement of the Vitality Score with a reduction in the Frailty Index. These findings further support the use of LAV-BPIFB4 gene therapy to induce beneficial effects on epigenetic mechanisms associated with aging and frailty in aged mice, with potential implications for future therapies to prevent frailty in humans.

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Section: Discussionmentioning

confidence: 99%

Effects of Human LAV-BPIFB4 Gene Therapy on the Epigenetic Clock and Health of Aged Mice

Giuliani

Barbi

Bigossi

et al. 2023

IJMS

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“…B20020585). As previously described, the preparation of Bazi Bushen has been described in sufficient detail 21‐25 . The chemical components of Bazi Bushen are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…The dosage of Bazi Bushen capsules (2.8 g kg −1 ) was based on the team's previous research data and literature reports. [21][22][23][24][25] The SAMR1 group and SAMP6 group were orally administrated with sterile saline. The mice in the BZBS group were orally administrated with the same volume of 2.8 g kg −1 of Bazi Bushen for 9 weeks.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

“…Bazi Bushen has been demonstrated to improve lipid metabolism, atherogenusis, aging-related hypogonadism, and aging-associated cognitive impairments. [21][22][23][24][25] Clinical studies have proved that it can mitigate the symptoms of patients with kidney essence deficiency, increase vitality, and improve the sexual function of impotence patients. However, there are few reports on the potential pharmacological functions of Bazi Bushen in maintaining intestinal homeostasis and regulating intestinal inflammation and gut microbiota.…”

Section: Introductionmentioning

confidence: 99%

“…The whole formula can tonify kidney, replenish essence, balance yin and yang, and delay aging. Bazi Bushen has been demonstrated to improve lipid metabolism, atherogenusis, aging‐related hypogonadism, and aging‐associated cognitive impairments 21‐25 . Clinical studies have proved that it can mitigate the symptoms of patients with kidney essence deficiency, increase vitality, and improve the sexual function of impotence patients.…”

Section: Introductionmentioning

confidence: 99%

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Bazi Bushen maintains intestinal homeostasis through inhibiting TLR4/NFκB signaling pathway and regulating gut microbiota in SAMP6 mice

Man

Gong

et al. 2023

J Sci Food Agric

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BACKGROUNDBazi Bushen is a Chinese patented medicine with multiple health benefits and geroprotective effects, yet, no research has explored its effects on intestinal homeostasis. In this study, we aimed to investigate the effect of Bazi Bushen on intestinal inflammation and the potential mechanism of gut microbiota dysbiosis and intestinal homeostasis in senescence‐accelerated mouse prone 6 (SAMP6). The hematoxylin and eosin (H&E) staining and Immunohistochemistry were performed to assess the function of the intestinal mucosal barrier. The enzyme‐linked immunosorbent assay (ELISA) and western blotting were used to determine the level of intestinal inflammation. The aging‐related β‐galactosidase (SA‐β‐gal) staining and western blotting were used to measure the extent of intestinal aging. The 16S ribosomal RNA (16S rRNA) was performed to analyze the change in gut microbiota composition and distribution.RESULTSBazi Bushen exerted remarkable protective effects in SAMP6, showing a regulated mucosal barrier and increased barrier integrity. It also suppressed intestinal inflammation through downregulating pro‐inflammatory cytokines (IL‐6, IL‐1β, and TNF‐α) and inhibiting TLR4/NFκB signaling pathway (MYD88, p‐p65, and TLR4). Bazi Bushen improved intestinal aging by reducing the area of SA‐β‐gal‐positive cells and the expression of senescence markers p16, p21, and p53. In addition, Bazi Bushen effectively rebuilt the gut microbiota ecosystem by decreasing the abundance of Bacteroides and Klebsiella, whiles increasing the ratio of Lactobacillus/Bacteroides and the abundance of Akkermansia.CONCLUSIONOur study shows that Bazi Bushen could serve as a potential therapy for maintaining intestinal homeostasis.This article is protected by copyright. All rights reserved.

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Role of epigenetics in the regulation of skin aging and geroprotective intervention: A new sight

Pozzo,

Xu,

Lin

et al. 2024

Biomedicine & Pharmacotherapy

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Bazi Bushen mitigates epigenetic aging and extends healthspan in naturally aging mice

Cited by 13 publications

References 56 publications

Effects of Human LAV-BPIFB4 Gene Therapy on the Epigenetic Clock and Health of Aged Mice

Effects of Human LAV-BPIFB4 Gene Therapy on the Epigenetic Clock and Health of Aged Mice

Bazi Bushen maintains intestinal homeostasis through inhibiting TLR4/NFκB signaling pathway and regulating gut microbiota in SAMP6 mice

Role of epigenetics in the regulation of skin aging and geroprotective intervention: A new sight

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