“…In yeast, decay rates were also determined with a temperature‐sensitive yeast strain that requires heat (or cold) shock that affects cell physiology (Grigull, Mnaimneh, Pootoolal, Robinson, & Hughes, ; Wang et al, ). Recently, several reports demonstrated that imaging technologies, such as smFISH (single molecule FISH) (Gómez‐Schiavon, Chen, West, & Buchler, ; Iyer, Park, & Kim, ; Raj, van den Bogaard, Rifkin, van Oudenaarden, & Tyagi, ; Trcek, Larson, Moldón, Query, & Singer, ), the MS2‐tagging system (Hocine, Raymond, Zenklusen, Chao, & Singer, ; Tutucci et al, ), and TREAT (Horvathova et al, ) can determine the kinetics of mRNAs by counting the absolute number of transcripts in single cells. These imaging technologies measure the rate of transcription and/or decay for specific transcripts, but these methods are typically restricted to a small number of mRNAs.…”