Bax and Bcl‐2 are involved in the apoptosis induced by local testicular heating in the boar testis

Hao, Xi Shan; Fan, Xue‐Gong; Zhang, Z; Liang, Yuan; Li, Q; He, Junlin

doi:10.1111/rda.12904

Cited by 13 publications

(9 citation statements)

References 36 publications

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“…Decreased numbers of PCNA and PLZF‐positive germ cells and expression levels suggest that the proliferation of spermatogonia and spermatocytes were mainly affected, which could explain the decrease in sperm concentration and implicate the affection of subsequent meiosis. Bax, which is involved in germ cell apoptosis, is up‐regulated when testis suffer from damage stress 21,22 . Results showed that CP caused a significant increase in TUNEL‐positive cell numbers of round and elongated spermatids, as well as Bax‐positive cells in spermatogonia, spermatocyte and elongated spermatids, which may regulate germ cell apoptosis and reflect the toxic effects of CP.…”

Section: Discussionmentioning

confidence: 98%

Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide

Liu

Wang

et al. 2021

J Cellular Molecular Medi

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Cyclophosphamide (CP) is a clinical anticancer drug that can cause male reproductive abnormalities, but the underlying mechanisms for this remain unknown. The present study aimed to explore the potential toxicity induced by CP in spermatogenesis events of germ cell proliferation, meiosis, and blood‐testis barrier integrity at the molecular level. CP‐treated mice showed significantly reduced serum testosterone levels, sperm motility and concentration. The results of immunohistochemistry and Western blot showed that CP reduced the proliferation of germ cells (PCNA, PLZF) and increased germ cell apoptosis (Bax and TUNEL‐positive cells) in CP‐treated mice testes. The expression of meiotic related proteins (SYCP3, REC8, MLH1) decreased significantly in the fourth week after administration, and the expression of blood‐testis barrier related proteins (β‐catenin, ZO‐1) and sperm quality‐associated proteins (PGK2, HSPA4) decreased significantly in the first week after administration. CP leads to the apoptosis of male germ cells, inhibits the proliferation of germ cells, and affects meiosis and the blood‐testis barrier, resulting in the decline of sperm quality. This study provides information to further the study of molecular mechanism and protective strategy of CP influence.

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Section: Discussionmentioning

confidence: 98%

Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide

Liu

Wang

et al. 2021

J Cellular Molecular Medi

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“…The Bcl-2 gene family serves a meditative and regulatory role in cell apoptosis (49). Key anti-apoptosis and pro-apoptosis factors in this family are Bcl-2 and Bax (50). As a proto-oncogene, Bcl-2 is a mitochondrial membrane protein and is considered the most important anti-apoptosis gene (51).…”

Section: Discussionmentioning

confidence: 99%

Protective functions of Lycium barbarum polysaccharides in H2O2‑injured vascular endothelial cells through anti‑oxidation and anti‑apoptosis effects

Xue

Zhu

et al. 2019

biom rep

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Cell injury in the cardiovascular endothelia caused by oxidative stress is among the major inducers of endothelium dysfunction and serves an important role in initiating cardiovascular diseases (CVDs). Therefore, protecting and improving the normal function of endothelial cells are considered key measures against CVDs. As a traditional Chinese medicinal component, Lycium barbarum is regarded to have high medicinal value. The present study aimed to investigate the potential anti-apoptosis and anti-oxidation effects of Lycium barbarum polysaccharides (LBPs) on injured rat artery endothelial cells, to demonstrate the experimental and medicinal values of LBPs. In the present study, the aortic endothelial cells of rats were cultivated and randomly divided into five groups: A control group, H2O2-injured group (H2O2 group), H2O2+LBPs (110 µg/ml) group (low-dose group, LT), H2O2+LBPs (220 µg/ml) group (medium-dose group, MT) and H2O2+LBPs (440 µg/ml) group (high-dose group, HT). Among these, the activity of superoxide dismutase (SOD), and the levels of malondialdehyde (MDA) and nitric oxide (NO) were detected by colorimetry. Additionally, the expression of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were detected by western blotting. It was observed that SOD activity and NO content decreased while MDA content increased significantly in the H2O2 group (P<0.05 vs. control); that SOD activity in the MT and HT group, and NO content in all three LBP groups were increased, while MDA content in the three LBP groups was decreased, compared with the H2O2 group (all P<0.05); that Bcl-2 expression decreased significantly in the H2O2 group while the expression of Bax increased significantly compared with the control group (both P<0.05); and that Bcl-2 expression in all three LBP groups increased, while Bax expression in the MT and HT groups decreased compared with the H2O2 group (all P<0.05), with these altered Bax levels being statistically similar to those in the control group (P>0.05). On light microscopy, the cells in the control group exhibited spindle-shaped morphology, consistent sizes, defined boundaries, and distinct nuclei of equivalent sizes with round or oval morphology. Additionally, the chromatin in the nuclei was evenly distributed, and all cells were adhered in a paving-stone arrangement. Notably, only few cells died. Conversely, the cells in the H2O2 group exhibited signs of damage and enlarged gaps, and focal cells died. In the HT group, the cells once again appeared adherent and exhibited similar morphological status to the normal cells. Overall, these results indicate that LBPs serve a protective role in oxidative-injured vascular endothelial cells through anti-apoptosis and anti-oxidation effects.

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“…Three boars were treated with local scrotal exposure to 42°C for approximately 1 hr with a home‐made electric blanket of controlled temperature as local scrotal heating group (Local scrotal heating), the other three boars received no heat treatment and were left at standard room temperature as control group (Control). Heating of the scrota of the boars was performed as described by our laboratory previous study (Xi et al., ). After 1 hr of heat treatment, the thermo‐controlled blanket for scrotal heating was removed, and the three boars were returned to their rooms.…”

Section: Methodsmentioning

confidence: 99%

Effect of local scrotal heating on the expression of tight junction‐associated molecule Occludin in boar testes

Zhang

Fan

Hao

et al. 2018

Reprod Domestic Animals

Self Cite

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The aim of this study was to determine whether local scrotal heating (42°C, for 1 hr) had an effect on the expression of tight junction (TJ)-associated molecule Occludin in boar testes. Adult boars (Landrace, n = 6) were used and randomly divided into two groups (n = 3 each). Three boars were given local scrotal exposure to 42°C for approximately 1 h with a home-made electric blanket of controlled temperature as local scrotal heating group, the other three boars received no heat treatment and were left at standard room temperature as control group. After 6 hr, all boars were castrated and the testes were harvested. qRT-PCR, Western blotting and immunohistochemistry were used to explore the expression and localization of Occludin. qRT-PCR and Western blotting showed that the protein and mRNA levels of Occludin significantly decreased in local scrotal heating group as compared to the control. Furthermore, immunoreactivity staining of Occludin was localized at the sites of the blood-testis barrier (BTB) and formed an almost consecutive and strong immunoreactivity strand in the control, while Occludin was limited to Sertoli cells (SCs) and no obvious immunoreactivity strand was present in local scrotal heating group. These data indicated that local scrotal heating decreased the expression of TJ-associated molecule Occludin, which may be involved in heat-induced spermatogenesis damage.

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Bax and Bcl‐2 are involved in the apoptosis induced by local testicular heating in the boar testis

Cited by 13 publications

References 36 publications

Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide

Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide

Protective functions of Lycium barbarum polysaccharides in H2O2‑injured vascular endothelial cells through anti‑oxidation and anti‑apoptosis effects

Effect of local scrotal heating on the expression of tight junction‐associated molecule Occludin in boar testes

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