Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

Silva, Mariana C.C.; Paula, Cláudia Alessandra Andrade de; Ferreira, Joana Gasperazzo; Paredes‐Gamero, Edgar Julian; Vaz, Angela M.S.F.; Sampaio, Misako U.; Correia, Maria T.S.; Oliva, Maria Luíza Vilela

doi:10.1016/j.bbagen.2014.03.009

Cited by 41 publications

(25 citation statements)

References 55 publications

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“…Plants used in folk medicine have been considered an important source of compounds with pharmacological activities, including antitumour. B. forficata is traditionally used in Brazil for treating cancer; nonetheless few studies concerning the cytotoxic properties of this plant have been carried out (Lim et al 2006;Gomes de Melo et al 2011;Silva et al 2014). In this work, the cytotoxic properties both of B. forficata leaves crude extract and FRF against two different tumour cell lines have been evaluated.…”

Section: Introductionmentioning

confidence: 96%

Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract

Miceli

Buongiorno

Celi

et al. 2015

Natural Product Research

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Bauhinia forficata Link. is utilised as an antidiabetic in Brazilian folk-medicine; furthermore, its antioxidant properties suggest a potential usefulness in the prevention of diabetes complications associated with oxidative stress. The contribution of a flavonoid-rich fraction (FRF), HPLC-PDA-ESI-MS characterised, to the antioxidant and cytotoxic properties of B. forficata hydro-alcoholic leaves extract was evaluated for the first time. Both extract and FRF showed radical-scavenging activity and reducing power with a strong relationship with the flavonoid content found; hence, flavonoids are mainly responsible for the primary antioxidant activity of B. forficata extract. The extract significantly decreased FO-1 cell viability at the higher concentrations. FRF did not exert any effect; thus, flavonoids do not appear to be responsible for the cytotoxicity of the extract. The extract resulted virtually non-toxic against both Artemia salina and normal human lymphocytes, demonstrating potential selectivity in inhibiting cancer cell growth. Finally, no antimicrobial activity was observed against the bacteria and yeasts tested.

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Section: Introductionmentioning

confidence: 96%

Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract

Miceli

Buongiorno

Celi

et al. 2015

Natural Product Research

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“…Such low Dox uptake from solution is attributed to possible Pgp efflux which limits intracellular Dox concentrations (Dong & Mumper, 2010). The very high Dox uptake of Pul NPs (11-fold enhancement compared with Dox solution) was possibly mediated by endocytosis through integrin receptors known to be expressed on MCF 7 cells (Silva et al, 2014;Singh et al, 2015).…”

Section: Pes Gantrezmentioning

confidence: 99%

Polyethylene sebacate doxorubicin nanoparticles: role of carbohydrate anchoring on in vitro and in vivo anticancer efficacy

et al. 2016

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We report carbohydrate-anchored polyethylene sebacate (PES)-Gantrez Õ AN 119 Doxorubicin hydrochloride (Dox) nanoparticles (NPs) for enhanced anticancer efficacy. The carbohydrates Arabinogalactan (AGn), an adjuvant in anticancer chemotherapy and pullulan (Pul) reported to promote collagen synthesis, were selected as ligands. PES Dox NPs of an average size around 200 nm, greater than 20% w/w Dox loading and negative zeta potential were anchored with Pul, AGn, and Pul-AGn combination by simple incubation. Increase in particle size and zeta potential confirmed carbohydrate anchoring. FTIR confirmed ionic complexation of Dox and Gantrez Õ AN 119. DSC and XRD demonstrated amorphization of Dox. Higher Dox release in pH 5.5 as compared with pH 7.4 is beneficial for reduced systemic toxicity and enhanced drug release in tumors. Good in vitro serum stability and low hemolysis revealed suitability for intravenous administration. All NPs revealed circulation longevity in normal rats. Pul NPs revealed superior anticancer efficacy in vitro and an 11-fold enhancement in uptake in MCF-7 breast cancer cells. The greater efficacy in vivo is attributed to possible pullulan-mediated integrin receptor uptake and interaction with tumor collagen. Histopathology confirmed safety and suggested promise of Pul NPs in improved anticancer efficacy.

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“…candida alba Buch.-Ham flowers. Nevertheless, the effect of a lectin isolated from B. forficata was studied and showed strong cytotoxic effects against MCF-7 cells; at 5 and 10 μM, the extracts were able to cause cells death after 24 and 48 h, respectively (Silva et al, 2014). By analysing the irradiation effects, the applied doses did not significantly affect the activity, except for the NCI-H460 cell line, in which the activity increased with the dose of 1 kGy.…”

Section: Effects Of Electron Beam Irradiation On the Bioactive Propermentioning

confidence: 99%

The influence of electron beam radiation in the nutritional value, chemical composition and bioactivities of edible flowers of Bauhinia variegata L. var. candida alba Buch.-Ham from Brazil

et al. 2018

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A B S T R A C TAs edible flowers are highly perishable, irradiation technology can be applied to increase their shelf life, as also for phytosanitary purposes. Herein, flowers of Bauhinia variegata L. var. candida alba Buch.-Ham were submitted to electron beam irradiation at the doses of 0.5, 0.8 and 1 kGy, to study the effects in the nutritional and chemical profiles, and also in antioxidant, cytotoxic and anti-inflammatory activities. The petals of white flowers revealed interesting bioactive properties being kaempferol derivatives the most abundant compounds, especially kaempferol-3-O-rutinoside. The applied irradiation doses did not highly affect the nutritional profile. No changes were produced in cytotoxicity, but the anti-inflammatory activity slightly decreased. However, the antioxidant activity was increased, especially in the dose of 0.5 kGy, in agreement with the higher content in phenolic compounds found at this dose.

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Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

Cited by 41 publications

References 55 publications

Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract

Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract

Polyethylene sebacate doxorubicin nanoparticles: role of carbohydrate anchoring on in vitro and in vivo anticancer efficacy

The influence of electron beam radiation in the nutritional value, chemical composition and bioactivities of edible flowers of Bauhinia variegata L. var. candida alba Buch.-Ham from Brazil

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