The main aim of the study was to find out whether cultural tourism could be a driver of rural development in the selected area and in general. In case yes, to what extent and under what conditions. Three districts in the South-Moravian Region, Znojmo, Břeclav, and Hodonín, situated in the rural borderland with Austria and Slovakia represented the study area. Both geographical and sociological methods were used to gather evidence for cultural tourism in that study. Firstly, attractiveness analysis of the area defined for cultural tourism took place. Next, factors influencing the potential for cultural tourism affecting rural development in South Moravia were evaluated. Finally, synergistic relations were discussed. In the territory, many forms of tourism intersect. Based on the results, it can be stated that cultural tourism can hardly be the main driver of rural development after the decline of agriculture because the region’s economy has branched out in several directions. However, it can be an important complementary activity that yields both economic and non-economic benefits.