Batch purification of high-purity lysozyme from egg white and characterization of the enzyme modified by PEGylation

Freitas, Débora da Silva; Abrahão-Neto, José

doi:10.3109/13880200903196859

Cited by 13 publications

(3 citation statements)

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“…6 Although our choice of a 5-kDa PEG is smaller than the latest favorites of the industry, protein drugs such as Adagen R , Oncaspar R , and Somavert R are modified using 5 kDa PEGs. 4,7 Numerous studies have investigated the PEGylation process, its effect on biological activity and circulation time, [8][9][10][11][12][13][14] and the effect of PEGylation on the protein thermal stability. 8,15,16 There are, however, surprisingly few studies that report the impact of the PEGylation on the pharmaceutical formulation development.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Protein PEGylation on Physical Stability in Liquid Formulation

Holm

McUmber

Rasmussen

et al. 2014

Journal of Pharmaceutical Sciences

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Section: Introductionmentioning

confidence: 99%

The Effect of Protein PEGylation on Physical Stability in Liquid Formulation

Holm

McUmber

Rasmussen

et al. 2014

Journal of Pharmaceutical Sciences

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“…The enzymatic activity of the protein–polymer conjugates was determined against substrate M. Lysodeikticus. ,, Remarkably, lysozyme–PEGMA and lysozyme–PGMA still provided intense enzymatic activity, which was between 70 and 90% of the corresponding activity of the untreated naked lysozyme tested under the same conditions (Figure ). These results showed that the PEGMA and PGMA conjugation did not cause substantial alterations in protein stability and accessibility of the active binding site.…”

Section: Resultsmentioning

confidence: 92%

Selective Protein Conjugation of Poly(glycerol monomethacrylate) and Poly(polyethylene glycol methacrylate) with Tunable Topology via Reductive Amination with Multifunctional ATRP Initiators for Activity Preservation

et al. 2022

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In this study, we compare poly(glycerol monomethacrylate) (PGMA) of different chain lengths and architectures (linear and two-arm) with poly(poly(ethylene glycol) methyl ether methacrylate) (PPEGMA) as an alternative polymer platform for the synthesis of a new generation of protein–polymer conjugates. Mono- and two-arm functional atom-transfer radical polymerization (ATRP) initiators were designed and selectively attached to lysozyme at the N-terminus via reductive amination. Site-specific, grafting from activator regenerated by electron transfer (ARGET) ATRP was carried out in phosphate buffer, and the reaction parameters were optimized to obtain polymer conjugates with predetermined molar mass and topology. The activity preservation under proteolytic and high-temperature conditions showed a clear dependence on the structure of the repeating unit and on the macromolecular architecture. These results highlighted the potential of PGMA as a poly(ethylene glycol) (PEG) alternative for the half-life extension of biotherapeutics. Moreover, this synthetic approach may inspire the design of a new class of protein–polymer conjugates through an optimal combination of macromolecular composition and topology.

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“…Similar results were found upon PEGylation of trichosanthin, which showed prolonged plasma half-life and reduced immunogenicity (He et al, 1999). PEGylation of lysozyme similarly lead to stabilization of the protein against pH and temperature variation as well as resistance against proteolysis (Silva Freitas & Abrahão-Neto, 2010). Most of these studies have been carried out in the context of pharmaceutical application.…”

Section: Pegylationmentioning

confidence: 99%

Advances in Chemical Engineering

Marin¹

2011

Advances in Chemical Engineering

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Dr Zeeshan Nawaz received his Bachelor and Master degree's with distinction in Chemical Engineering from MUET Jamshoro and UET Lahore, respectively. He earned his Doctoral degree in Chemical Engineering & Technology from Tsinghua University, Beijing in 2010. He was engaged in number of process design assignments at Chemical Process & Plant Design Center, Islamabad. Later he worked at Beijing Key Laboratory for Green Chemical Reaction Engineering & Technology (FLOTU), on catalyst design and fluidization engineering. He won many awards and schallorships in his carrier, the most recents are Excellent Researcher Award of Tsinghua University 2009 sponsored by Eternal Chemical Co. Ltd., and Best Young Scientist Award 2011 by National Association of Young Scientists. Currently, he is working as Scientist in SABIC Technology Center, Saudi Basic Industries Corporation, Riyadh, and involved in the high fidelity reactor modeling and vatious technology development activities. Dr. Zeeshan is also member of editorial boards for various reputed research journals.Prof Dr Shahid Naveed born in Pakistan received his Doctoral degree in Chemical Engineering in 1987 from University of Newcastle, UK. He is associated with academic and research work in Chemical Engineering in the country and abroad for more than 35 years. He has served at the University of North Carolina at Charlotte, USA on a Post Doctoral Fulbright Fellowship. His current research interests relates to energy with focus on coal gasification. At present he is serving as Chairman Chemical Engineering Department at UET, Lahore Pakistan. He is the winner of HEC Best Teacher Award 2010. Dr. Naveed is Team Leader of Gasification Research group and is supervising a number of R&D projects.

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Batch purification of high-purity lysozyme from egg white and characterization of the enzyme modified by PEGylation

Cited by 13 publications

References 54 publications

The Effect of Protein PEGylation on Physical Stability in Liquid Formulation

The Effect of Protein PEGylation on Physical Stability in Liquid Formulation

Selective Protein Conjugation of Poly(glycerol monomethacrylate) and Poly(polyethylene glycol methacrylate) with Tunable Topology via Reductive Amination with Multifunctional ATRP Initiators for Activity Preservation

Advances in Chemical Engineering

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