Batch procedure for thermal elution of DNA from hydroxyapatite

Brenner, Don J.; Fanning, G R; Rake, A. V.; Johnson, Karl

doi:10.1016/0003-2697(69)90199-7

Cited by 220 publications

(79 citation statements)

References 11 publications

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“…The change in absorbance of each sample was followed as the temperature was increased at a rate of 0.5"C/min. (3,4). Samples diluted to 0.12 M PB were added to 1.0 ml of freshly prepared hydroxyapatite equilibrated in the same buffer at 65°C.…”

Section: Methodsmentioning

confidence: 99%

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Brown

Vickers

1980

International Journal of Systematic Bacteriology

106

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DNA homology studies were performed with strains of Legionella pneumophila Brenner et al., 1979, including the type strain, ATCC 33152, and a group of seven previously unclassified bacteria which resemble L. pneumophila in growth characteristics and colonial morphology and by their content of large amounts of branched-chain fatty acids. The organisms studied include three isolates of the Pittsburgh pneumonia agent (PPA), TATLOCK, WIGA (ALLOI), MI-15 (ALLOZ), and NY-23 (ALLOJ. The purified DNAs were labeled with 32P in vitro by a nick translation method, which made it possible to achieve very high specific activities. Results of our hybridization experiments suggest that these previously unclassified organisms are not related to L. pneumophila at the genus level, and thus two new genera within the family Legionellaceae are proposed for them: Tatlockia and Fluoribacter. The type species of these genera are T. micdadei (Hebert et al., 1980) comb. nov. and F. bozemanae sp. nov., respectively. The type strain of 2 ' . micdadei is strain TATLOCK (= ATCC 33218), and the type strain of F. bozemanae is strain WIGA (= ATCC 33217).Legionnaires' disease, first recognized during an outbreak in Philadelphia in 1976 (9), has been shown to be due to infection with a bacterium (15) for which the name Legionella pneumophila has been proposed (5). This organism is gram negative and requires cysteine, iron salts, and a medium buffered at pH 6.9 for optimal growth (8).Since the isolation of L. pneumophila, intensive study of previously unclassified organisms (1, 10, 16, 24) and a search for new isolates (7, 12, 14, 21, 25) from both human and environmental sources has led to the recognition of a number of organisms resembling L. pneumophila with respect to nutritional requirements and colonial morphology (7, 12, 14). Of these organisms, L. pneurnophila, WIGA (ALLOI), Pittsburgh pneumonia agent (PPA), HEBA, and TATLOCK possess common flagellar antigens (10, 12), a relationship which may exist among all flagellated members of this group of organisms and which may be of taxonomic importance. Cellular fatty acid analysis disclosed the presence of branched-chain fatty acids in unusually large amounts for gram-negative organisms (19), and characteristic fatty-acid patterns were described for L. pneumophila and for several others of these bacteria (7, 10, 14, 18, 19).In this article, we present extensive deoxyribonucleic acid (DNA)-DNA homology data in an attempt to determine the relatedness among members of this group. Since the original submission of this manuscript for publication, two articles have appeared proposing scientific names for the organisms referred to as TAT-LOCK and PPA (11,20); one of the articles also refers to the name L. bozemanii for the WIGA bacterium, but a formal proposal is not made (11). Both sets of authors have placed these organisms in the genus Legionella. Our data, as well as the available data presented in these other reports, do not support the classification of these organisms into a single genus, but arg...

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Section: Methodsmentioning

confidence: 99%

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Brown

Vickers

1980

International Journal of Systematic Bacteriology

106

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“…After incubation, samples were diluted to 0.14 M phosphate buffer, and sodium dodecyl sulfate was added to 0.4%. Reassociated DNA was separated from unreacted DNA on hydroxyapatite by use of a batch procedure (5). Unreacted DNA did not bind to hydroxyapatite.…”

Section: Meat South Carolinamentioning

confidence: 99%

Deoxyribonucleic Acid Relatedness Between Serratia plymuthica and Other Serratia Species, with a Description of Serratia odorifera sp. nov. (Type Strain: ICPB 3995)

Grimont

Richard

et al. 1978

International Journal of Systematic Bacteriology

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Seven new isolates of Serratiaplymuthica (including two from a human source) and 24 isolates resembling unclustered Serratia strain 38 (Grimont et al., J. Gen. Microbiol98:39-66, 1977) are described. Deoxyribonucleic acid relatedness studies, obtained with labeled reference deoxyribonucleic acid from s. plymuthica 392 confirm that S. plymuthica is a discrete species. Mean percent relatedness of S. plymuthica isolates to strain 392 was 88 f 13.6 (standard deviation), whereas the mean percent relatedness of S. Ziquefaciens isolates (the closest species) to strain 392 was 52 f 12.4. Strain 38 and twenty-four "38-like" isolates constitute a new deoxyribonucleic acid hybridization group that is 28 to 43% related to Serratia species and to the "Citrobacter-like" group of Leclerc and Buttiaux, and 15 to 22% related to other known species of Enterobacteriaceae. This 38-like group constitutes a new species that is named Serratia odorifera sp. nov. (type strain, ICPB 3995). Two biotypes (1 and 2) are described. Strains of this species will grow on caprylate-thallous agar (selective for Serratia spp.), and they have a characteristic odor. Most strains of S. odorifera were recovered from clinical specimens.

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“…DNA was extracted and purified as described previously (Brenner et al, 1972). The procedures for labelling of DNA with tritium-labelled nucleotides and for hybridization experiments (S1 nuclease treatment, trichloroacetic acid procedure) have been detailed elsewhere (Grimont et al, 1980 ;Khammas et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

Scola¹,

Mallet²,

Grimont³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Batch procedure for thermal elution of DNA from hydroxyapatite

Cited by 220 publications

References 11 publications

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Deoxyribonucleic Acid Relatedness Between Serratia plymuthica and Other Serratia Species, with a Description of Serratia odorifera sp. nov. (Type Strain: ICPB 3995)

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

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