Batch foam separation of a soluble protein

To clarify oil-in-water (o/w) emulsion, flotation experiments were conducted by adding methylated milk casein (MeCS), which is a biodegradable flocculant. Emulsion used in this study was prepared by ultrasonic emulsification of heavy oil (bunker-A) and sodium dodecyl sulfate (SDS) solution. It was found that addition of MeCS enhanced clarification of oil droplets from o/w emulsion solution due to floc formation by adding MeCS. An optimum dosage of MeCS to form effective floc was determined by the relative turbidity in a clarification experiment. In flotation experiments, clarification ability was evaluated by removal rate constant, k , obtained by fitting of turbidity data to a pseudo-first-order kinetic equation. In case of varying dosage of MeCS and keeping superficial gas velocity, U g , at constant value (5.48 × 10 −2 cm/s), removal rate was greatly influenced by amount of MeCS dosage. The maximum value of k was also obtained at the most optimum dosage of MeCS, and k decreased with adding lesser or larger amount of MeCS than the optimum dosage. On the other hand, in case of keeping the dosage of MeCS at the optimum dosage, and varying superficial gas velocity, up to U g < ca. 0.2 cm/s, k was increased with increasing superficial gas velocity, and increase in removal rate of oil droplets was mostly proportional with increase in bubble surface area production rate. 

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“…[ 17] A bubble column of 4.4 cm inner diameter and 0.4 m in height was employed. The column was made of transparent acrylic resin.…”

Section: Flotation Experimentsmentioning

confidence: 99%

Enhancement of oil droplet removal from o/w emulsion by adding methylated milk casein in flotation technique

Maruyama

Tada

Seki

2009

Asia-Pacific J Chem Eng

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“…This accessible technique has been studied in many fields, such as chemical/biochemical engineering, analytical chemistry, and wastewater treatment (Maruyama et al, 2007). In principle, the strong amphipathic nature of proteins and enzymes, with polar and non-polar groups, causes them to be preferentially adsorbed at the gas-liquid interface and foam fractionation can be used to separate and to concentrate such proteins (Aksay and Mazza, 2007).…”

Section: Introductionmentioning

confidence: 99%

A novel method for hydrophobin extraction using CO2 foam fractionation system

Khalesi¹,

Venken

Deckers³

et al. 2013

Industrial Crops and Products

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“…Pre-purification steps may improve the yield of the final product. Foam fractionation is an accessible method for enriching the proteins in a solution with potential of lowering the costs of downstream processing [58,59]. The latter contributes almost 60 % of the total cost of production [60,61].…”

Section: Hydrophobin Pre-purification Using Foam Fractionationmentioning

confidence: 99%

Recent Advances in Fungal Hydrophobin Towards Using in Industry

2015

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Fungal hydrophobin is a family of low molecular weight proteins consisting of four disulfide bridges and an extraordinary hydrophobic patch. The hydrophobic patch of hydrophobins and the molecules of gaseous CO2 may interact together and form the stable CO2-nanobubbles covered by an elastic membrane in carbonated beverages. The nanobubbles provide the required energy to provoke primary gushing. Due to the hydrophobicity of hydrophobin, this protein is used as a biosurfactant, foaming agent or encapsulating agent in food products and medicine formulations. Increasing demands for using of hydrophobins led to a challenge regarding production and purification of this product. However, the main issue to use hydrophobin in the industry is the regulatory affairs: yet there is no approved legislation for using hydrophobin in food and beverages. To comply with the legislation, establishing a consistent method for obtaining pure hydrophobins is necessary. Currently, few research teams in Europe are focusing on different aspects of hydrophobins. In this paper, an up-to-date collection of highlights from those special groups about the bio-chemical and physicochemical characteristics of hydrophobins have been studied. The recent advances of those groups concerning the production and purification, positive applications and negative function of hydrophobin are also summarised.

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Batch foam separation of a soluble protein

Cited by 40 publications

References 42 publications

Enhancement of oil droplet removal from o/w emulsion by adding methylated milk casein in flotation technique

Enhancement of oil droplet removal from o/w emulsion by adding methylated milk casein in flotation technique

A novel method for hydrophobin extraction using CO2 foam fractionation system

Recent Advances in Fungal Hydrophobin Towards Using in Industry

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