Batch culture of Candida utilis in a medium deprived of a phosphorous source

Callieri, D. A. S.; Núñez, Carlos Germán; Ricci, Juan C. Díaz; Scida, L. A.

doi:10.1007/bf00251849

Cited by 9 publications

(1 citation statement)

References 7 publications

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“…The diminished cellular protein suggests that impaired protein synthesis may be crucial to trigger ASX synthesis in P. rhodozyma cells that are grown under low initial phosphate concentrations. Moreover, both impairments in the synthesis of proteins and nucleic acids, and diminished growth, have been reported as common outcomes when growing yeasts in phosphate-deficient media (Boer et al, 2010 ; Callieri et al, 1984 ).…”

Section: Factors That Encourage Asx Synthesis In P Rhodozymamentioning

confidence: 99%

Deciphering the mechanism by which the yeastPhaffia rhodozymaresponds adaptively to environmental, nutritional, and genetic cues

Flores-Cotera

Chávez-Cabrera

Martínez-Cárdenas

et al. 2021

Journal of Industrial Microbiology and Biotechnology

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Phaffia rhodozyma is a basidiomycetous yeast that synthesizes astaxanthin (ASX), which is a powerful and highly valuable antioxidant carotenoid pigment. P. rhodozyma cells accrue ASX and gain an intense red-pink coloration when faced with stressful conditions such as nutrient limitations (e.g. nitrogen or copper), the presence of toxic substances (e.g. antimycin A), or are affected by mutations in the genes that are involved in nitrogen metabolism or respiration. Since cellular accrual of ASX occurs under a wide variety of conditions, this yeast represents a valuable model for studying the growth conditions that entail oxidative stress for yeast cells. Recently, we proposed that ASX synthesis can be largely induced by conditions that lead to reduction-oxidation (redox) imbalances, particularly the state of the NADH/NAD+ couple together with an oxidative environment. In this work, we review the multiple known conditions that elicit ASX synthesis expanding on the data that we formerly examined. When considered alongside the Mitchell's chemiosmotic hypothesis, the study served to rationalize the induction of ASX synthesis and other adaptive cellular processes under a much broader set of conditions. Our aim was to propose an underlying mechanism that explains how a broad range of divergent conditions converge to induce ASX synthesis in P. rhodozyma. The mechanism that links the induction of ASX synthesis with the occurrence of NADH/NAD+ imbalances may help in understanding how other organisms detect any of a broad array of stimuli or gene mutations, and then adaptively respond to activate numerous compensatory cellular processes.

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Section: Factors That Encourage Asx Synthesis In P Rhodozymamentioning

confidence: 99%

Deciphering the mechanism by which the yeastPhaffia rhodozymaresponds adaptively to environmental, nutritional, and genetic cues

Flores-Cotera

Chávez-Cabrera

Martínez-Cárdenas

et al. 2021

Journal of Industrial Microbiology and Biotechnology

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Kinetics of growth and phosphate uptake in pure culture studies of Acinetobacter species

Hao

Chang

1987

Biotech & Bioengineering

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Acinetobacter has been found to be the major species responsible for mediating biological phosphate removal. The growth kinetics and phosphate uptake were investigated for an isolated Acinetobacter strain growing in a defined medium. The phosphate uptake is dependent on growth rate, temperature, and pH. Polyphosphate granules occurred in a balanced growth stage. The maximum phosphorus content in cells was 4.8% at the dilution rate of 12 day(-1). The specific phosphate uptake rate was found to be a quadratic polynomial function of the dilution rate. Increased calcium (up to 36 mg/L) and magnesium (up to 15 mg/L), and the addition of yeast extract (100 mg/L), primary effluent (20%), and fluoride (10 mg/L) did not affect phosphate uptake. Anaerobic conditioning (N(2) stripping), low pH (CO(2) stripping), and addition of sodium acetate under anaerobic conditions failed to stimulate immediate phosphate release. Nevertheless, After 21-24 h, the phosphate release was ca. 3, 5, and 15 mg P/g cell, respectively, for N(2) purging, the addition of acetate, and CO(2) purging. For two-stage completely stirred reactor operation, there was negligible phosphate overplus at the second reactor when phosphate was added, when the first reactor was subjected to phosphate limitation. When both phosphate and carbon limited the growth in the first reactor, there was slight phosphate accumulation under endogenous respiration conditions in the second reactor.

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Determination of the optimal conditions for the continuous culture of Candida utilis in sugarcane stillage

Ricci

Callieri

Garro

1987

Appl Microbiol Biotechnol

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Batch culture of Candida utilis in a medium deprived of a phosphorous source

Cited by 9 publications

References 7 publications

Deciphering the mechanism by which the yeastPhaffia rhodozymaresponds adaptively to environmental, nutritional, and genetic cues

Deciphering the mechanism by which the yeastPhaffia rhodozymaresponds adaptively to environmental, nutritional, and genetic cues

Kinetics of growth and phosphate uptake in pure culture studies of Acinetobacter species

Determination of the optimal conditions for the continuous culture of Candida utilis in sugarcane stillage

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