Batch culture enrichment of ANAMMOX populations from anaerobic and aerobic seed cultures

Suneethi, S.; Joseph, Kurian

doi:10.1016/j.biortech.2010.07.121

Cited by 21 publications

(8 citation statements)

References 16 publications

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“…In addition, different types of inoculating sludge lead to a certain difference in the tolerance to nitrogen loading and thereafter different performance stability. Suneethi et al enriched anammox populations from anaerobic and aerobic seed cultures in batch mode and found that anaerobic seed showed better stability and ammonia removal efficiency than the aerobic seed (Suneethi and Joseph 2011).…”

Section: Discussionmentioning

confidence: 98%

“…Several types of seeding sludge from various wastewater treatment processes have been studied by previous researchers, including sewage sludge, nitrifying sludge, denitrifying sludge, anaerobic granular sludge, and the sediments from freshwater and marine environments (Dalsgaard et al 2003;Hong et al 2011a;Ikuo Tsushima 2007;Suneethi and Joseph 2011;Tsushima et al 2007). Mature granules and the enriched anammox sludge were more likely to start an anammox reactor in a short time (Hu et al 2011;Liao et al 2008;Ni et al 2011).…”

Section: Introductionmentioning

confidence: 98%

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Anammox start-up in sequencing batch biofilm reactors using different inoculating sludge

Yang

Gao

2012

Appl Microbiol Biotechnol

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In this study, anammox bacteria were rapidly enriched in sequencing batch biofilm reactors (SBBRs) with different inoculations. The activated sludge taken from a sequencing batch reactor was used and inoculated to SBBR1, while SBBR2 was seeded with stored anaerobic sludge from an upflow anaerobic fixed bed (2-year stored at 5-15 °C). Nitrogen removal performance, anammox activity, biofilm characteristics and variation of the microbial community were evaluated. The maximum total nitrogen loading rate (NLR) of SBBR1 gradually reached to 1.62 kgN/(m³/day) with a removal efficiency higher than 88% and the NLR of SBBR2 reached to 1.43 kgN/(m³/day) with a removal efficiency of 86%. SBBR2 was more stable compared to SBBR1. These results, combined with molecular techniques such as scanning electron microscope, fluorescence in situ hybridization, and terminal restriction fragment length polymorphism, indicated that different genera of anammox bacteria became dominant. This research also demonstrates that SBBR is a promising bioreactor for starting up and enriching anammox bacteria.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Anammox start-up in sequencing batch biofilm reactors using different inoculating sludge

Yang

Gao

2012

Appl Microbiol Biotechnol

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“…The SBR technique has been fruitfully applied in many laboratories , although other approaches to culture these organisms have also been developed. Bioreactors such as upflow-anaerobic sludge blanket (UASB) reactors (Strous et al, 1998;Imajo et al, 2004;Schmidt et al, 2004a,b;Ni et al, 2010), rotating biological contactors (Van de Graaf et al, 1996;Egli et al, 2001Egli et al, , 2003Windey et al, 2005), and even manually fed batch cultivation systems (S anchez-Melsi o et al, 2009;Suneethi & Joseph, 2011;Yasuda et al, 2011) were used. All methods, however, rely on the settling properties of anammox cell aggregates.…”

Section: Anammox Bacteriamentioning

confidence: 99%

How to make a living from anaerobic ammonium oxidation

et al. 2013

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Anaerobic ammonium-oxidizing (anammox) bacteria primarily grow by the oxidation of ammonium coupled to nitrite reduction, using CO2 as the sole carbon source. Although they were neglected for a long time, anammox bacteria are encountered in an enormous species (micro)diversity in virtually any anoxic environment that contains fixed nitrogen. It has even been estimated that about 50% of all nitrogen gas released into the atmosphere is made by these 'impossible' bacteria. Anammox catabolism most likely resides in a special cell organelle, the anammoxosome, which is surrounded by highly unusual ladder-like (ladderane) lipids. Ammonium oxidation and nitrite reduction proceed in a cyclic electron flow through two intermediates, hydrazine and nitric oxide, resulting in the generation of proton-motive force for ATP synthesis. Reduction reactions associated with CO2 fixation drain electrons from this cycle, and they are replenished by the oxidation of nitrite to nitrate. Besides ammonium or nitrite, anammox bacteria use a broad range of organic and inorganic compounds as electron donors. An analysis of the metabolic opportunities even suggests alternative chemolithotrophic lifestyles that are independent of these compounds. We note that current concepts are still largely hypothetical and put forward the most intriguing questions that need experimental answers.

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“…ORP value was a good indicator for providing more detailed information on monitoring the performance and operational state of an anammox reactor [26]. It was reported that the anammox process could take place in an ORP range of 50-140 mv [24,[26][27]. TN removal did not occur until the second hour, probably due to the impact of high DO content, and it then increased gradually to 84.4% at hour 12.…”

Section: Variations Of Water Quality Characteristics In R1 During Onementioning

confidence: 99%

Enhancing Nitrogen Removal Performance in a Bioreactor Using Immobilized Anaerobic Ammonium Oxidation Sludge by Polyvinyl Alcohol-Sodium Alginate (PVA-SA)

Lu¹,

Ma²,

Liang³

et al. 2018

Pol. J. Environ. Stud.

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Anaerobic ammonium oxidation (anammox) is an efficient and promising pathway for nitrogen removal from wastewater, but its application is usually confined by the low growth rate and susceptibility to surroundings of anammox bacteria. In this study, cultured anammox sludge was immobilized using polyvinyl alcohol (PVA)-sodium alginate (SA) gel and put into a lab-scale column reactor at a packing ratio of 20%, and nitrogen removal performance was evaluated at two hydraulic retention times (HRTs). Anammox was rapidly initiated in the reactor, with ammonium and nitrite removal efficiency reaching 82.3% and 84.7% after an operation period of 10 d. Nitrogen removal efficiency declined greatly after the reduction of HRT from 24 h to 12 h, but then recovered quickly, with an average TN removal rate of 84.5% and 0.43 kg·m -3 ·d -1 achieved under the steady operational state. The immobilized anammox reactor performed significantly better and was more stable in nitrogen removal than that with anammox sludge inoculated directly, indicating the superiority of cell entrapment of anammox biomass in addition to its easy reservation. Nitrogen removal in the reactor increased after stable operation with the HRT. It has potential to apply immobilized anammox sludge entrapped by PVA-SA gel for the convenient establishment of an anammox reactor with stable and high nitrogen removal rates.

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Batch culture enrichment of ANAMMOX populations from anaerobic and aerobic seed cultures

Cited by 21 publications

References 16 publications

Anammox start-up in sequencing batch biofilm reactors using different inoculating sludge

Anammox start-up in sequencing batch biofilm reactors using different inoculating sludge

How to make a living from anaerobic ammonium oxidation

Enhancing Nitrogen Removal Performance in a Bioreactor Using Immobilized Anaerobic Ammonium Oxidation Sludge by Polyvinyl Alcohol-Sodium Alginate (PVA-SA)

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