Batch and continuous 2‐heptanone production by Ca‐alginate/Eudragit RL entrapped spores of Penicillium roquefortii

Abstract

The synthesis of 2-heptanone from octanoic acid by Caalginate/Eudragit RL entrapped spores of Penicillium roquefortii is performed in batch and continuous reactions. The measurement of the HCI solution needed to overcome the pH increase during the course of a reaction allows continuous monitoring of the reaction progress of batch as well as continuous processes without any aliquot sampling. The hydration of the biocatalyst prior to a bioconversion performance is of major importance to achieve reproducible results. A continuous reaction carried out at pH 6.5 for one month demonstrates the feasibility of such a process and shows that the reaction occurs with a Michaelian behavior, with K(M) = 1.82 mmol/L and r(sm) = 0.82 mmol/L h, and no intraparticle diffusion limitation is found. The complex behavior of the spores during batch reactions makes this kind of operation unsuitable for kinetic studies.

Biotransformation of myrcene by Pseudomonas aeruginosa

“…They are important chemical constituents of the essential oil of many plants, with widespread applications in folk medicine and in aromatherapy [1] .…”

Behavior of spores of Penicillium roquefortii during fed‐batch bioconversion of octanoic acid into 2‐heptanone

“…This system proved very useful for investigating the behavior of the biocatalyst during long-term bioconversions without the need for cell immobilization, as is the case in continuous operations. 26 In this article, we report results on biocatalyst activity and stability when it is used in this way.…”

Conidial production by Penicillium nalgiovense for use as starter cultures in dry fermented sausages by solid state fermentation

“…Even though there are reports on conidia production by SSF most of them are referred to biocontrol. Spore production by fungi used as starter in foods is scarce, being predominantly referred to Penicillium roqueforti and its application to the production of different cheeses (Larroche & Gros, 1989). Also many of them are under patent (Mora & Pintus, 2008;Raimbault & Germon, 1976).…”

“…Spore production by Penicillium has been reported on natural substrates such as buckwheat seeds (Larroche & Gros, 1989), maize and paddy (Cahagnier, Lesage, & Molard, 1993). In Argentina soy, maize and wheat are the three most important crops and therefore easy to obtain at low cost for its use as substrate in SSF.…”