2008
DOI: 10.1002/cyto.b.20419
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Basophil activation test by flow cytometry: Present and future applications in allergology

Abstract: The diagnosis of allergic reactions in clinical practice rests upon both clinical history and the demonstration of specific immunoglobulin E (sIgE), either in the serum or via skin tests. However, for various reasons, identification of the offending allergen(s) is not always possible. Moreover, not all allergies are IgE-mediated. In an attempt to find reliable methods to investigate hypersensitivity reactions, histamine and sulfidoleukotriene release tests have long been introduced. However, relatively few com… Show more

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Cited by 152 publications
(137 citation statements)
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References 84 publications
(92 reference statements)
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“…Th e assay currently uses fl ow cytometry to detect upregulation, aft er antigen stimulation, of some activation markers (e.g., CD63 or CD203c) usually expressed on the cell surface, in order to identify activated basophils ( 31 ).…”
Section: The Usefulness Of Basophil Activation Assay In Ncws Diagnosismentioning
confidence: 99%
“…Th e assay currently uses fl ow cytometry to detect upregulation, aft er antigen stimulation, of some activation markers (e.g., CD63 or CD203c) usually expressed on the cell surface, in order to identify activated basophils ( 31 ).…”
Section: The Usefulness Of Basophil Activation Assay In Ncws Diagnosismentioning
confidence: 99%
“…In PMA þ ionomycin experiments, cells were first incubated with PMA for 10 min and subsequently with ionomycin for an additional 10 min. A stimulation time of 20 min was employed in order to allow comparison with CD63 and CD230c (21,22).…”
Section: Ige-independent Activation With Fmlp Pma and Ionomycinmentioning
confidence: 99%
“…Cuando existe contacto de un basófilo sensibilizado con un alérgeno específico que se une a la IgE, los basófilos secretan una amplia variedad de mediadores bioactivos y generan la expresión de marcadores de activación en su membrana como CD63 y CD203c que tras la activación celular se expresan en la membrana citoplasmática, lo cual permite su medición mediante citometría de flujo utilizando anticuerpos monoclonales específicos Anti CD63 y CD203c marcados con fluorocromos. Los mejores resultados de esta técnica se han visto cuando se hace diagnóstico de alergias a medicamentos [36][37][38] .…”
Section: Discussionunclassified