Basics of Molecular Genetic Mapping and QTL Analysis in Plants

Kumar, J.; Pratap, Aditya; Solanki, Ramesh

doi:10.2174/978160805090111001010035

Cited by 5 publications

(7 citation statements)

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Section: Marker–trait Associationmentioning

confidence: 99%

“…Then, genotypic data and the phenotypic data recorded on target traits over the environments are used to establish marker–trait association using computer software (e.g. QTL CATOGRAPHER, J oinmap ) (see Kumar et al. 2009b for details).…”

Section: Marker–trait Associationmentioning

confidence: 99%

“…For this purpose, a biparental mapping population developed from parents having contrasting traits is required, which may be an F 2 , BC (backcross) and DH (doubled haploid) population. A mapping population consisting of 50-200 individuals is genotyped with a number of markers polymorphic between parents (Kumar et al 2009b). If the target trait is measured as discrete trait, marker-trait association can be established on the basis of expected and observed recombination frequency using chi-square test, and genetic distance between marker and trait can be measured with or without preparing the genetic linkage maps.…”

Section: Genetic Linkage Mappingmentioning

confidence: 99%

“…Then, genotypic data and the phenotypic data recorded on target traits over the environments are used to establish marker-trait association using computer software (e.g. QTL CATOGRAPHER, JOINMAP) (see Kumar et al 2009b for details). Both framework and dense molecular maps comprising more than one type of molecular markers have been constructed in the past years in chickpea (Winter et al 2000), cowpea , lentil (Hamwieh et al 2005) and other pulse crops listed in a recent review (Varshney et al 2010b).…”

Section: Genetic Linkage Mappingmentioning

confidence: 99%

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Towards marker‐assisted selection in pulses: a review

Kumar¹,

Choudhary²,

Solanki³

et al. 2011

Plant Breeding

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160

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Pulses are important sources of proteins in vegetarian diet. However, genetic improvement in production and productivity of pulse crops has been very slow owing to several constraints. The present view of researchers is that the effectiveness and efficiency of conventional breeding can be significantly improved by using molecular markers. Nowadays, molecular markers are routinely utilized worldwide in all major crops as a component of breeding. The pace of development of molecular markers and other genomic sources has been accelerated in chickpea, pigeon pea and some other pulses, and marker-trait associations have been established for a number of important agronomic traits. The efforts are underway to use high-throughput genotyping platforms besides developing more genomic resources in other pulses. So far, progress in the use of marker-assisted selection as a part of pulse breeding programmes has been very slow and limited to few pulse crops such as chickpea and common bean. In this article, we have reviewed the progress made, limitations encountered and future possibilities for the application of marker-assisted selection in the genetic improvement of pulse crops.

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Section: Marker–trait Associationmentioning

confidence: 99%

Section: Marker–trait Associationmentioning

confidence: 99%

Section: Genetic Linkage Mappingmentioning

confidence: 99%

Section: Genetic Linkage Mappingmentioning

confidence: 99%

See 2 more Smart Citations

Towards marker‐assisted selection in pulses: a review

Kumar¹,

Choudhary²,

Solanki³

et al. 2011

Plant Breeding

Self Cite

160

View full text Add to dashboard Cite

show abstract

“…Markers linked to desirable genes or QTLs are important tools for improving plants in marker assisted breeding (MAB), via marker-assisted selection (MAS) and marker-assisted backcrossing (MABC). Map design is the first step towards positional cloning of genes responsible for a particular phenotype, with ultra-dense genetic maps required for this type of research [ 52 , 53 ].…”

Section: Introductionmentioning

confidence: 99%

How Machine Learning Methods Helped Find Putative Rye Wax Genes Among GBS Data

Góralska

Bińkowski

Lenarczyk

et al. 2020

IJMS

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The standard approach to genetic mapping was supplemented by machine learning (ML) to establish the location of the rye gene associated with epicuticular wax formation (glaucous phenotype). Over 180 plants of the biparental F2 population were genotyped with the DArTseq (sequencing-based diversity array technology). A maximum likelihood (MLH) algorithm (JoinMap 5.0) and three ML algorithms: logistic regression (LR), random forest and extreme gradient boosted trees (XGBoost), were used to select markers closely linked to the gene encoding wax layer. The allele conditioning the nonglaucous appearance of plants, derived from the cultivar Karlikovaja Zelenostebelnaja, was mapped at the chromosome 2R, which is the first report on this localization. The DNA sequence of DArT-Silico 3585843, closely linked to wax segregation detected by using ML methods, was indicated as one of the candidates controlling the studied trait. The putative gene encodes the ABCG11 transporter.

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Auxin increased adventitious root development in the medicinal plant Andrographis paniculata (Burm. f.) Wall. ex Nees

2021

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Andrographis paniculata (Burm. f.) Wall. ex Nees is an economically high‐demand medicinal plant for its traditional uses and overwhelming pharmacological properties. However, the current agricultural production of A. paniculata is insufficient to meet its pharmacological demand. Developing a rapid and efficient propagation technique is crucial to meet its increasing demand. This study assessed the ability of the auxins indole‐3‐butyric acid (IBA) and indole‐3‐acetic acid (IAA) to simulate adventitious root development in the young apical shoot (YAS) and old apical shoot (OAS) cuttings of A. paniculata. This investigation found that 3.0 mM IBA was better for achieving rapid growth (6.33 ± 0.42 d) of adventitious root in YAS cuttings, where the number of roots per cutting was 40 ± 1.29, with an average length of 4.11 ± 0.20 cm (mean ± SE; n = 6). Overall, YAS cuttings were comparatively more efficient to induce adventitious roots compared with OAS cuttings. The rapid regeneration of A. paniculata plantlets using IBA within a short time suggests that this plant can be propagated on a mass scale and that IBA can be used to accelerate production. Further study for evaluating soaking duration on the adventitious rooting in A. paniculata would be worthy.

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Basics of Molecular Genetic Mapping and QTL Analysis in Plants

Cited by 5 publications

References 0 publications

Towards marker‐assisted selection in pulses: a review

Towards marker‐assisted selection in pulses: a review

How Machine Learning Methods Helped Find Putative Rye Wax Genes Among GBS Data

Auxin increased adventitious root development in the medicinal plant Andrographis paniculata (Burm. f.) Wall. ex Nees

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