Basics and Biomedical Applications of Dielectric Barrier Discharge (DBD)

Bibinov, Nikita; Rajasekaran, Priyadarshini; Wandke, Philipp Mertmann Dirk; Viöl, Wolfgang; Awakowicz, Peter

doi:10.5772/13192

Cited by 14 publications

(17 citation statements)

References 36 publications

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“…Plasma was produced between the insulated high-voltage electrode and the grounded base holding the cell sample, which acted as the other electrode. Similar DBD plasmas have gas and surface temperatures of around 478C [78,79]. To ensure that plasma-induced heating did not damage cells, we measured the temperature change in 200 ml phosphatebuffered saline (PBS) or serum-free medium postplasma treatment using a thermistor probe and meter (YSI 427 and 43TA, Yellow Springs Instruments).…”

Section: Plasma Treatmentmentioning

confidence: 99%

Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species

Arjunan

Friedman

Fridman

et al. 2011

J. R. Soc. Interface.

168

100

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Vascularization plays a key role in processes such as wound healing and tissue engineering. Nonthermal plasma, which primarily produces reactive oxygen species (ROS), has recently emerged as an efficient tool in medical applications including blood coagulation, sterilization and malignant cell apoptosis. Liquids and porcine aortic endothelial cells were treated with a non-thermal dielectric barrier discharge plasma in vitro. Plasma treatment of phosphate-buffered saline (PBS) and serum-free medium increased ROS concentration in a dose-dependent manner, with a higher concentration observed in serum-free medium compared with PBS. Species concentration inside cells peaked 1 h after treatment, followed by a decrease 3 h post treatment. Endothelial cells treated with a plasma dose of 4.2 J cm -2 had 1.7 times more cells than untreated samples 5 days after plasma treatment. The 4.2 J cm -2 plasma dose increased two-dimensional migration distance by 40 per cent compared with untreated control, while the number of cells that migrated through a three-dimensional collagen gel increased by 15 per cent. Tube formation was also enhanced by plasma treatment, with tube lengths in plasma-treated samples measuring 2.6 times longer than control samples. A fibroblast growth factor-2 (FGF-2) neutralizing antibody and ROS scavengers abrogated these angiogenic effects. These data indicate that plasma enhanced proliferation, migration and tube formation is due to FGF-2 release induced by plasma-produced ROS. Non-thermal plasma may be used as a potential tool for applying ROS in precise doses to enhance vascularization.

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Section: Plasma Treatmentmentioning

confidence: 99%

Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species

Arjunan

Friedman

Fridman

et al. 2011

J. R. Soc. Interface.

168

100

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“…This can be seen in Figure c, where a long exposure shows a generally homogeneous discharge with additional stable channels. Very similar images were taken of a DBD ignited in air on inhomogeneous surfaces of human and mouse skins …”

Section: Resultsmentioning

confidence: 99%

“…Very similar images were taken of a DBD ignited in air on inhomogeneous surfaces of human and mouse skins. [36,37] After the DBD treatment, we study the surface of the silicon wafer in the electron microscope. On the treated surface, we observe several splinters of different dimensions.…”

Section: Resultsmentioning

confidence: 99%

Micro‐plasmoids in self organized filamentary dielectric barrier discharges

Engelhardt

Kogelheide

Stapelmann

et al. 2016

Plasma Processes & Polymers

Self Cite

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A filamentary dielectric barrier discharge (DBD) is ignited on a silicon wafer under atmospheric pressure conditions in a mixture of argon and air (0.5/0.5) in two different modes, namely a stochastically ignited filamentary discharge and a self‐organized filamentary discharge by the application of high voltage (HV) pulses at two repetition frequencies, 0.5 and 5 kHz. The discharge conditions are characterized by optical emission spectroscopy and current–voltage measurements. The silicon wafer surface treated with the DBD is studied with an electron microscope. The formation of a homogeneous silicon oxide layer is observed after treatment under a stochastically filamentary DBD. Whereas, in the self‐organized filamentary DBD, etching tracks (thin channels) and blisters are produced on the silicon wafer surface, which are interpreted as tracks of plasmoids, namely plasma objects without any direct contact to a power supply. The transition between the different filamentary modes of the DBD plasma occurs in the presented study through an increase of the repetition frequency of HV pulses, but it can also be caused by small silicon splinters on the wafer surface. The splinters cause ignitions in stable positions, and therefore induce a combination of discharge modes, namely stochastically and self‐organized DBD mode. In close proximity to the splinters, tracks of plasmoids are observed, even in the DBD at low frequency.

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“…The plasma has a low temperature (up to 45°C). The sample is several millimeters from plasma source and active species directly treat sample [19].…”

Section: Plasma Dischargesmentioning

confidence: 99%

Microplasma Drug Delivery

Shimizu¹,

Krištof²

2018

Plasma Medicine - Concepts and Clinical Applications

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There are several techniques to perform drug delivery. One of the newest methods of drug delivery through the skin is the application of plasma. Reactive species generated by plasma can change the chemical composition of the skin, change the structure and extract lipids of the lipid barrier, create pores in or etch the surface of the skin. These changes have an influence on the barrier function of the skin which can be decreased. The main barrier of the skin is called stratum corneum. The structure and composition of the stratum corneum and function of the components is described. Possible interaction of plasma particles with skin is presented and compared with interaction of plasma species with carbon or hydrocarbon surfaces. Active species which can effectively interact with lipid molecules is introduced. Hydrophilic drugs and drugs with high molecular weight can penetrate very difficult through the skin or cannot penetrate the skin at all. As a model, a drug, Cyclosporine A, was studied. Cyclosporine A is a lipophilic drug with a molecular weight of 1203 Da which is used during and after organ transplantation to prevent rejection. The Hairless Yucatan micropig was used to simulate human skin. A film electrode was used to generate plasma that was used for skin treatment. An AC voltage (V 0-p = 0.6-1.5 kV, 25 kHz) was applied with flowing gas (5 L/min). The barrier function of the skin was evaluated by a Franz cell experiment and high performance liquid chromatography (HPLC) for a particular drug. An effective amount of drug in human body was determined by pharmacokinetic model.

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Basics and Biomedical Applications of Dielectric Barrier Discharge (DBD)

Cited by 14 publications

References 36 publications

Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species

Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species

Micro‐plasmoids in self organized filamentary dielectric barrier discharges

Microplasma Drug Delivery

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