Basic transcription element binding protein is a thyroid hormone‐regulated transcription factor expressed during metamorphosis in <i>Xenopus laevis</i>

Hoopfer, Eric D.; Huang, Liyue; Denver, Robert J.

doi:10.1046/j.1440-169x.2002.00650.x

Cited by 37 publications

(47 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TH, the major effect of which is through the T 3 -mediated signaling pathway, regulates a great many cellular physical activities including respiration (42)(43)(44). In the present study, accumulation of cellular fumarate was confirmed as a phenomenon characteristic of T 3 -induced tricarboxylic acid cycle dysfunction.…”

Section: T 3 Induces Intracellular Fumarate Accumulation By Affectingsupporting

confidence: 66%

Mechanism of Cancer Cell Adaptation to Metabolic Stress

Bai

et al. 2009

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Gastric cancer is the second most common cancer worldwide and has a poor prognosis. To determine the mechanism of adaptation to metabolic stress in cancer cells, we used gastric cancer as a model system to reveal the potential signaling pathways involved. Two-dimensional polyacrylamide gel electrophoresis coupled with ESI-Q-TOF MS/MS analysis was used to identify differentially expressed proteins between gastric tumor tissues and the corresponding noncancerous tissues. In total, 107 spots with significant alteration (؎over 2-fold, p < 0.05) were positively identified by MS/MS analysis. Altered expression of representative proteins was validated by RT-PCR and Western blotting. Cluster analysis of the changed proteins revealed an interesting group of metabolic proteins, which suggested accumulation of triiodothyronine (T 3 ; the major functional component of thyroid hormone) and overexpression of hypoxia-induced factor (HIF) in gastric carcinoma. These observations were further confirmed by electrochemiluminescence immunoassay and immunohistochemistry. T 3 -induced expression of HIF1-␣ and vascular endothelial growth factor was further verified using a gastric cancer cell line and in vivo mouse model. Because the early accumulation of HIF1-␣ was found to be independent of de novo transcription, we also found that the cytosolic cascade phosphatidylinositol 3-kinase/Akt pathway sensitive to T 3 stimulus was involved. Furthermore we demonstrated that T 3 -induced overexpression of HIF1-␣ was mediated by fumarate accumulation and could be enhanced by fumarate hydratase inactivation but inhibited by 2-oxoglutarate. These results provide evidence for alteration of metabolic proteins and dysfunction of thyroid hormone regulation in gastric tumors, and a novel thyroid hormone-mediated tumorigenic signaling pathway is proposed. Our findings are considered a significant step toward a better understanding of adaptations to metabolic stress in gastric carcinogenesis. Molecular & Cellular Proteomics 8: 70 -85, 2009. Cellular hypoxia and metabolic stress have been observed in many cancer types (1-3). Hypoxia-induced factor (HIF)1 is considered a central regulator of oxygen homeostasis. HIF1-␣ or HIF2-␣, together with HIF1-␤, forms an active transcription complex that controls numerous target genes, which have roles in glycolysis, angiogenesis, and tumor metastasis (4). Previous studies indicated that HIF1-␣ is overexpressed in many cancer types, such as colon, lung, renal, and thyroid gland, whereas HIF proteins mediate cell adaptation to hypoxia (1-3). Both HIF1-␣ and HIF2-␣ are labile under normoxic conditions; this is because of proteasomal degradation following their oxygen-dependent ubiquitination by ubiquitin ligase complex targeted to HIF by the von Hippel-Lindau protein (VHL) (5, 6). VHL recognition of HIF1-␣ requires the enzymatic hydroxylation of two converted residues on HIF1-␣ mediated by HIF prolyl hydroxylase (HPH) (7,8). HPH activity requires the cofactors ascorbate and iron and the cosubstrates 2-oxoglutatrate a...

show abstract

Section: T 3 Induces Intracellular Fumarate Accumulation By Affectingsupporting

confidence: 66%

Mechanism of Cancer Cell Adaptation to Metabolic Stress

Bai

et al. 2009

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…Bteb has been shown to mediate T 3 -induced neural differentiation and neurite branching, and these effects on neurogenesis occur via TH activation of trb (Denver et al 1999, Cayrou et al 2002. In Xenopus, bteb mRNA has been shown to be upregulated by T 3 in brain and other tissues during metamorphosis (Furlow & Kanamori 2002, Hoopfer et al 2002, while in mammals, T 3 upregulation of bteb appears specific to neurons and is only seen during early developmental stages (Denver et al 1999). In both amphibian and mammalian tissues, bteb appears to act as an immediate-early gene with T 3 upregulation of bteb transcripts occurring rapidly (within 24-72 h; Hoopfer et al 2002).…”

Section: Discussionmentioning

confidence: 99%

“…In Xenopus, bteb mRNA has been shown to be upregulated by T 3 in brain and other tissues during metamorphosis (Furlow & Kanamori 2002, Hoopfer et al 2002, while in mammals, T 3 upregulation of bteb appears specific to neurons and is only seen during early developmental stages (Denver et al 1999). In both amphibian and mammalian tissues, bteb appears to act as an immediate-early gene with T 3 upregulation of bteb transcripts occurring rapidly (within 24-72 h; Hoopfer et al 2002). By contrast, we observed a decline in bteb transcript abundance in the brain of male and female minnows after 10 days of T 3 treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The bteb gene encodes a zinc-fingered transcription factor that binds GC-box domains to regulate TH-mediated gene transcription. Previously, bteb has been shown to be TH regulated in the brain of mice (Denver et al 1999) and in the brain, intestine, and tail of Xenopus (Furlow & Kanamori 2002, Hoopfer et al 2002, but it has not been tested whether bteb is TH regulated in the neural tissues of adult teleosts.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Thyroid hormone regulation of mRNAs encoding thyrotropin β-subunit, glycoprotein α-subunit, and thyroid hormone receptors α and β in brain, pituitary gland, liver, and gonads of an adult teleost, Pimephales promelas

Lema¹,

Dickey²,

Schultz³

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

Thyroid hormone regulation of mRNAs encoding thyrotropin b-subunit, glycoprotein a-subunit, and thyroid hormone receptors a and b in brain, pituitary gland, liver, and gonads of an adult teleost, Pimephales promelas AbstractThyroid hormones (THs) regulate growth, morphological development, and migratory behaviors in teleost fish, yet little is known about the transcriptional dynamics of gene targets for THs in these taxa. Here, we characterized TH regulation of mRNAs encoding thyrotropin subunits and thyroid hormone receptors (TRs) in an adult teleost fish model, the fathead minnow (Pimephales promelas). Breeding pairs of adult minnows were fed diets containing 3,5, 3 0 -triiodo-L-thyronine (T 3 ) or the goitrogen methimazole for 10 days. In males and females, dietary intake of exogenous T 3 elevated circulating total T 3 , while methimazole depressed plasma levels of total thyroxine (T 4 ). In both sexes, this methimazole-induced reduction in T 4 led to elevated mRNA abundance for thyrotropin b-subunit (tshb) in the pituitary gland. Fish treated with T 3 had elevated transcript levels for TR isoforms a and b (tra and trb) in the liver and brain, but reduced levels of brain mRNA for the immediate-early gene basic transcription factor-binding protein (bteb). In the ovary and testis, exogenous T 3 elevated gene transcripts for tshb, glycoprotein hormone a-subunit ( gpha), and trb, while not affecting tra levels. Taken together, these results demonstrate negative feedback of T 4 on pituitary tshb, identify tra and trb as T 3 -autoinduced genes in the brain and liver, and provide new evidence that tshb, gpha, and trb are THs regulated in the gonad of teleosts. Adult teleost models are increasingly used to evaluate the endocrine-disrupting effects of chemical contaminants, and our results provide a systemic assessment of TH-responsive genes during that life stage.

show abstract

“…Expression analysis for candidate TH-response genes in the intestine during metamorphosis (SHH) have been demonstrated to be TH-response genes in X. laevis (Fini et al, 2012a;Hasebe et al, 2011;Heimeier et al, 2009). Their expression levels in the intestine parallel endogenous TH levels (Heimeier et al, 2010;Hoopfer et al, 2002;Wang et al, 2008). Changes in TH-response gene expression levels during metamorphosis can reflect to some degree the responsiveness of TH-response genes to TH.…”

Section: Animalsmentioning

confidence: 99%

A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus

Zhang

Qin

et al. 2015

Journal of Environmental Sciences

View full text Add to dashboard Cite

Amphibian metamorphosis provides a wonderful model to study the thyroid hormone (TH) signaling disrupting activity of environmental chemicals, with Xenopus laevis as the most commonly used species. This study aimed to establish a rapid and sensitive screening assay based on TH-response gene expression analysis using Pelophylax nigromaculatus, a native frog species distributed widely in East Asia, especially in China. To achieve this, five candidate TH-response genes that were sensitive to T3 induction were chosen as molecular markers, and T3 induction was determined as 0.2 nmol/L T3 exposure for 48 hr. The developed assay can detect the agonistic activity of T3 with a lowest observed effective concentration of 0.001 nmol/L and EC50 at around 0.118-1.229 nmol/L, exhibiting comparable or higher sensitivity than previously reported assays. We further validated the efficiency of the developed assay by detecting the TH signaling disrupting activity of tetrabromobisphenol A (TBBPA), a known TH signaling disruptor. In accordance with previous reports, we found a weak TH agonistic activity for TBBPA in the absence of T3, whereas a TH antagonistic activity was found for TBBPA at higher concentrations in the presence of T3, showing that the P. nigromaculatus assay is effective for detecting TH signaling disrupting activity. Importantly, we observed non-monotonic dose-dependent disrupting activity of TBBPA in the presence of T3, which is difficult to detect with in vitro reporter gene assays. Overall, the developed P. nigromaculatus assay can be used to screen TH signaling disrupting activity of environmental chemicals with high sensitivity.

show abstract

Basic transcription element binding protein is a thyroid hormone‐regulated transcription factor expressed during metamorphosis in Xenopus laevis

Cited by 37 publications

References 42 publications

Mechanism of Cancer Cell Adaptation to Metabolic Stress

Mechanism of Cancer Cell Adaptation to Metabolic Stress

Thyroid hormone regulation of mRNAs encoding thyrotropin β-subunit, glycoprotein α-subunit, and thyroid hormone receptors α and β in brain, pituitary gland, liver, and gonads of an adult teleost, Pimephales promelas

A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus

Contact Info

Product

Resources

About