Basic Performance of High Speed, High Purity and High Intensive Ball Mill

Tanno, Koichi; Satoh, Tomoaki; Maruyama, A.; Yanbe, Yuuichi; Fujitaka, Yuuji

doi:10.2497/jjspm.53.68

Cited by 5 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This chitin was named C0. Converge milling was conducted by milling 20 g α-chitin samples at 800 rpm with zirconia balls (10 mm in diameter) using a converge mill (Makabe giken Co. Ltd.; , ) . The volume of balls used in the experiment represented 10% of the sample volume.…”

Section: Materials and Methodsmentioning

confidence: 99%

Conversion of α-Chitin Substrates with Varying Particle Size and Crystallinity Reveals Substrate Preferences of the Chitinases and Lytic Polysaccharide Monooxygenase of Serratia marcescens

Nakagawa

Eijsink

Totani

et al. 2013

J. Agric. Food Chem.

View full text Add to dashboard Cite

1Industrial depolymerization of chitinous biomass generally requires numerous steps and the 2 use of deleterious substances. Enzymatic methods provide an alternative, but fundamental 3 knowledge that could direct potential development of industrial enzyme cocktails is scarce. 4We have studied the contribution of mono-component chitinases (ChiA, -B, and -C) and the 5 lytic polysaccharide monooxygenase (LPMO) from Serratia marcescens on depolymerization 6 of -chitin substrates with varying particle size and crystallinity that were generated using a 7 converge mill. For all chitinases activity was positively correlated to a decline in particle size 8 and crystallinity. Especially ChiC, the only non-processive endo-chitinase from the S. 9 marcescens chitinolytic machinery, benefited from mechanical pretreatment. Combining the 10 chitinases revealed clear synergies for all substrates tested. CBP21, the chitin-active LPMO 11 from S. marcescens, increased solubilization of substrates with high degrees of crystallinity 12 when combined with each of the three chitinases, but this synergy was reduced upon decline 13 in crystallinity. 14 15 16 KEYWORDS 17 -chitin, mechanical pretreatment, chitinase, GH18, lytic polysaccharide monooxygenases, 18

show abstract

Section: Materials and Methodsmentioning

confidence: 99%