Basic leucine zipper transcription factors C/EBP and MafL in the hydrozoan jellyfish <i>Podocoryne carnea</i>

Seipel, Katja; Yanze, Nathalie; Müller, Peter; Streitwolf, Ruth; Schmid, Volker

doi:10.1002/dvdy.20061

Cited by 7 publications

(5 citation statements)

References 53 publications

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“…5 ), a region corresponding to early stages of nematogenesis in hydrozoan medusae ( Denker et al 2008 ). Expression patterns here suggest that these genes might have a role inducing cell proliferation as they are expressed in highly proliferative regions of the developing and/or adult medusa ( Spring et al 2000 ; Seipel, Yanze, et al 2004 ; Denker et al 2008 ). These spatially restricted expression patterns were not observed in H. symbiolongicarpus sporosacs ( fig.…”

Section: Resultsmentioning

confidence: 88%

“…Several previous studies, using a candidate gene approach, identified genes specific to medusae development in P. carnea ( Schuchert et al 1993 ; Aerne et al 1995 ; Gröger et al 1999 ; Masuda-Nakagawa et al 2000 ; Müller et al 1999 ; Yanze et al 1999 ; Spring et al 2000 ; Spring et al 2002 ; Müller et al 2003 ; Seipel, Yanze, et al 2004 ; Seipel et al 2004a –c; Stierwald et al 2004 ; Torras et al 2004 ; Galle et al 2005 ; Reber-Müller et al 2006 ). As additional validation of our DE results, we determined whether any of these genes were present in our pool of 938 candidates that were identified as significantly upregulated in developing gonophores and/or adult medusa ( table 3 ).…”

Section: Resultsmentioning

confidence: 99%

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Interspecific Differential Expression Analysis of RNA-Seq Data Yields Insight into Life Cycle Variation in Hydractiniid Hydrozoans

Sanders

Cartwright

2015

Genome Biol Evol

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Hydrozoans are known for their complex life cycles, which can alternate between an asexually reproducing polyp stage and a sexually reproducing medusa stage. Most hydrozoan species, however, lack a free-living medusa stage and instead display a developmentally truncated form, called a medusoid or sporosac, which generally remains attached to the polyp. Although evolutionary transitions in medusa truncation and loss have been investigated phylogenetically, little is known about the genes involved in the development and loss of this life cycle stage. Here, we present a new workflow for evaluating differential expression (DE) between two species using short read Illumina RNA-seq data. Through interspecific DE analyses between two hydractiniid hydrozoans, Hydractinia symbiolongicarpus and Podocoryna carnea, we identified genes potentially involved in the developmental, functional, and morphological differences between the fully developed medusa of P. carnea and reduced sporosac of H. symbiolongicarpus. A total of 10,909 putative orthologs of H. symbiolongicarpus and P. carnea were identified from de novo assemblies of short read Illumina data. DE analysis revealed 938 of these are differentially expressed between P. carnea developing and adult medusa, when compared with H. symbiolongicarpus sporosacs, the majority of which have not been previously characterized in cnidarians. In addition, several genes with no corresponding ortholog in H. symbiolongicarpus were expressed in developing medusa of P. carnea. Results presented here show interspecific DE analyses of RNA-seq data to be a sensitive and reliable method for identifying genes and gene pathways potentially involved in morphological and life cycle differences between species.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Interspecific Differential Expression Analysis of RNA-Seq Data Yields Insight into Life Cycle Variation in Hydractiniid Hydrozoans

Sanders

Cartwright

2015

Genome Biol Evol

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“…Its role in Drosophila eye development remains to be determined. A large Maf homologue, MafL, has been recently cloned from hydrozoan jellyfish (Seipel et al, 2004). Putative Maf binding sites were shown in the invertebrate squid SL11 and SL12, jellyfish J3-, and scallop omega-crystallin promoters (Carosa et al, 2002;Kozmik et al, 2003;Tomarev et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Large Maf Transcription Factors: Cousins of AP-1 Proteins and Important Regulators of Cellular Differentiation

Yang

Cvekl

2016

EJBM

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“…In fact members of the Six, Pax, bHLH, Sox, Pou gene classes are involved in both neurogenesis and myogenesis in bilaterians. In the Podocoryne jellyfish where both differentiation pathways were monitored during medusa budding and induced transdifferentiation, the analysis of the Six, C/EBP, MafL, Atonal like 1, Achaete-scute 2 genes (Table 1) as well as the observation of the transient expression of neuronal markers during myogenesis (Seipel et al, 2004b;Seipel et al, 2004c;Stierwald et al, 2004) suggested that muscle cells and nerve cells derive from common myoepithelial cells. These molecular data actually fit with the three steps model proposed by George Mackie for the origin of neuromuscular transmission (Mackie, 1970) whereby muscle cells and nerve cells would have diverged from myoepithelial cells (see Figure 5a in Arendt, 2008).…”

Section: What Was the Proto-neuronal Cell From Which Nerve Cells Evolmentioning

confidence: 99%

Origins of neurogenesis, a cnidarian view

Galliot

Quiquand

Ghila

et al. 2009

Developmental Biology

154

133

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New perspectives on the origin of neurogenesis emerged with the identification of genes encoding post-synaptic proteins as well as many "neurogenic" regulators as the NK, Six, Pax, bHLH proteins in the Demosponge genome, a species that might differentiate sensory cells but no neurons. However, poriferans seem to miss some key regulators of the neurogenic circuitry as the Hox/paraHox and Otx-like gene families. Moreover as a general feature, many gene families encoding evolutionarily-conserved signaling proteins and transcription factors were submitted to a wave of gene duplication in the last common eumetazoan ancestor, after Porifera divergence. In contrast gene duplications in the last common bilaterian ancestor, Urbilateria, are limited, except for the bHLH Atonal-class. Hence Cnidaria share with Bilateria a large number of genetic tools. The expression and functional analyses currently available suggest a neurogenic function for numerous orthologs in developing or adult cnidarians where neurogenesis takes place continuously. As an example, in the Hydra polyp, the Clytia medusa and the Acropora coral, the Gsx/cnox2/Anthox-2 ParaHox gene likely supports neurogenesis. Also neurons and nematocytes (mechanosensory cells) share in hydrozoans a common stem cell and several regulatory genes indicating that they can be considered as sister cells. Performed in anthozoan and medusozoan species, these studies should tell us more about the way(s) evolution hazards achieved the transition from epithelial to neuronal cell fate, and about the robustness of the genetic circuitry that allowed neuromuscular transmission to arise and be maintained across evolution.

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Basic leucine zipper transcription factors C/EBP and MafL in the hydrozoan jellyfish Podocoryne carnea

Cited by 7 publications

References 53 publications

Interspecific Differential Expression Analysis of RNA-Seq Data Yields Insight into Life Cycle Variation in Hydractiniid Hydrozoans

Interspecific Differential Expression Analysis of RNA-Seq Data Yields Insight into Life Cycle Variation in Hydractiniid Hydrozoans

Large Maf Transcription Factors: Cousins of AP-1 Proteins and Important Regulators of Cellular Differentiation

Origins of neurogenesis, a cnidarian view

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