Basic fibroblast growth factor modulates cell cycle of human umbilical cord‐derived mesenchymal stem cells

Ramasamy, Rajesh; Tong, Chih Kong; Yip, Wai Kien; Vellasamy, Shalini; Tan, Boon Chong; Seow, Heng Fong

doi:10.1111/j.1365-2184.2012.00808.x

Cited by 44 publications

(35 citation statements)

References 35 publications

(37 reference statements)

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“…Thus, we compared growth, phenotype, differentiation potential, metabolism, and longevity of UC-MSC cultured in xeno-free media to control cells propagated in standard serum-based medium (with 10 % FBS; M6) and serum-reduced medium (5 % FBS) supplemented with bFGF (5 ng/mL; M7), since bFGF has been shown to enhance proliferation of UC-MSCs [27]. A scheme of experimental procedures is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles

et al. 2016

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Growing evidence indicates that intracellular signaling mediated by extracellular vesicles (EVs) released by stem cells plays a considerable role in triggering the regenerative program upon transplantation. EVs from umbilical cord mesenchymal stem cells (UC-MSC-EVs) have been shown to enhance tissue repair in animal models. However, translating such results into clinical practice requires optimized EV collection procedures devoid of animal-originating agents. Thus, in this study, we analyzed the influence of xeno-free expansion media on biological properties of UC-MSCs and UC-MSC-EVs for future applications in cardiac repair in humans. Our results show that proliferation, differentiation, phenotype stability, and cytokine secretion by UC-MSCs vary depending on the type of xeno-free media. Importantly, we found distinct molecular and functional properties of xeno-free UC-MSC-EVs including enhanced cardiomyogenic and angiogenic potential impacting on target cells, which may be explained by elevated concentration of several pro-cardiogenic and pro-angiogenic microRNA (miRNAs) present in the EVs. Our data also suggest predominantly low immunogenic capacity of certain xeno-free UC-MSC-EVs reflected by their inhibitory effect on proliferation of immune cells in vitro. Summarizing, conscious selection of cell culture conditions is required to harvest UC-MSC-EVs with the optimal desired properties including enhanced cardiac and angiogenic capacity, suitable for tissue regeneration.Key message Type of xeno-free media influences biological properties of UC-MSCs in vitro.Certain xeno-free media promote proliferation and differentiation ability of UC-MSCs.EVs collected from xeno-free cultures of UC-MSCs are biologically active.Xeno-free UC-MSC-EVs enhance cardiac and angiogenic potential of target cells.Type of xeno-free media determines immunomodulatory effects mediated by UC-MSC-EVs. Electronic supplementary materialThe online version of this article (doi:10.1007/s00109-016-1471-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles

et al. 2016

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“…These findings are supported by our own data, clearly indicating acceleration of proliferation from the beginning of FGF-2 administration, and improved chondrogenic outcome for FGF-2 augmented groups. It has recently been shown that FGF-2 improves proliferation of umbilical cord-derived MSCs (UC-MSCs), but that their 'nature' remains unaffected, as reflected by immunophenotype and immunomodulatory properties (49), although it was also clearly stated that cytokine profiles were significantly altered by FGF administration. However, those authors noted that their cells met all criteria for MSCs defined by the International Society for Cellular Therapy (ISCT) (19), although these data were not shown (49).…”

Section: Discussionmentioning

confidence: 99%

FGF‐2 addition during expansion of human bone marrow‐derived stromal cells alters MSC surface marker distribution and chondrogenic differentiation potential

et al. 2013

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“…In the present study it was also observed that late EPCs cultured in EGM-2MV exhibit a greater number of apoptotic cells. Ramasamy et al (26) demonstrated that elevated expression of stem cell-associated transcription factors is correlated with a reduction in cell apoptosis. As EGM-2MV is the medium that most strongly supports the differentiation of EPCs towards endothelial cells, it is reasonable that a greater number of apoptotic cells were observed in late EPCs in the EGM-2MV medium.…”

Section: Discussionmentioning

confidence: 99%

Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media

Guan

Cheng

et al. 2013

Molecular Medicine Reports

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Ex vivo expansion of endothelial progenitor cells (EPCs) may be a promising strategy to overcome the clinical problem of limited cell numbers. As the culture medium is the key for the cell characteristics, the effects of different culture media on EPCs were investigated in the present study. Rat bone marrow mononuclear cells were cultured in different media, including M-199 media with 20% fetal bovine serum (FBS) and bovine pituitary extract (M1); M-199 media with 10% FBS, 20 ng/ml vascular endothelial growth factor (VEGF) and 10 ng/ml basic fibroblast growth factor (bFGF; M2) or epidermal growth medium (EGM)-2MV media. The cell morphology and biological functions, such as proliferation, adhesion, migration, tube formation and nitric oxide (NO) production were subsequently assayed in vitro. Moreover, endothelial biomarkers and apoptosis were also analyzed. The results showed that endothelial‑like cells appeared in all of the culture systems. First‑passage cells, namely early EPCs, tended to form colonies in M2 and EGM-2MV media but showed a fusiform shape in M1 media. The 3rd or 4th generation EPCs, namely late EPCs, cultured in EGM-2MV media exhibited increased adhesion, migration, tube formation and NO production as compared with EPCs in M1 or M2 media. Furthermore, late EPCs cultured in EGM-2MV expressed higher levels of endothelial cell markers, such as von Willibrand factor (vWF)and CD31, but relatively greater levels of apoptosis were observed. In conclusion, cell culture conditions, for example the medium used, affects the biological properties of bone marrow-derived early and late EPCs.

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Basic fibroblast growth factor modulates cell cycle of human umbilical cord‐derived mesenchymal stem cells

Abstract: Our study showed that bFGF supplementation of UC-MSC culture enhanced the cells' growth kinetics without compromising their nature.

Cited by 44 publications

References 35 publications

Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles

Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles

FGF‐2 addition during expansion of human bone marrow‐derived stromal cells alters MSC surface marker distribution and chondrogenic differentiation potential

Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media

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