Basic fibroblast growth factor is a morphogenic modulator in kidney vessel development

Kloth, Sabine; Gerdes, Johannes; Wanke, Christiane; Minuth, Will W.

doi:10.1046/j.1523-1755.1998.00854.x

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…Expression of bFGF in vessels might indicate a more mature vasculature being associated with low-grade tumors. Similar observations have been reported for other tumors like melanoma [40], and bFGF has been suggested to promote vascular maturation [29].…”

Section: Discussionsupporting

confidence: 84%

Expression of bFGF/FGFR-1 and vascular proliferation related to clinicopathologic features and tumor progress in localized prostate cancer

et al. 2005

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Microvessel density (MVD) has been associated with progression of prostate cancer. Although basic fibroblast growth factor (bFGF) is a known endothelial mitogen, the prognostic role of bFGF and its receptor FGFR-1 in prostate cancer has been controversial. The aim of our study was to examine the tissue distribution and prognostic significance of bFGF, FGFR-1, and microvascular proliferation. Sections from 104 radical prostatectomy specimens were examined by factor VIII/Ki-67 staining for proliferating capillary index (PCI) and MVD, and tissue microarray sections were immunostained for bFGF and FGFR-1. Increased PCI (median 0.49%) was related to strong stromal expression of bFGF (P=0.003) but was without prognostic impact. Strong bFGF staining was associated with well-differentiated tumors, no capsular penetration, low serum-prostate-specific antigen (s-PSA), low tumor cell proliferation, and increased time to biochemical failure (P=0.007), and was of independent prognostic importance in multivariate survival analysis. bFGF expression in vessels was associated with low MVD (P=0.0003). In contrast, strong tumor cell FGFR-1 expression was related to high preoperative s-PSA. Thus, increased stromal and vessel bFGF was associated with less aggressive tumors. Our findings indicate a complex relationship between bFGF/FGFR-1 expression and prognosis of prostate cancer. Vascular proliferation revealed no prognostic impact in this study.

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Section: Discussionsupporting

confidence: 84%

Expression of bFGF/FGFR-1 and vascular proliferation related to clinicopathologic features and tumor progress in localized prostate cancer

et al. 2005

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“…Many other growth factors expressed during nephrogenesis [1] affect the proliferation and migration of EC: these include fibroblast growth factor [25], hepatocyte growth factor [26,27] and transforming growth factor-β1 [28]. However, the actions of these factors are not confined to EC, and they have effects on epithelial, and other metanephric lineages [1].…”

Section: The Tie-1 Rtk In Kidney Ec Differentiationmentioning

confidence: 99%

Angiopoietin growth factors and Tie receptor tyrosine kinases in renal vascular development

Woolf

Yuan

2001

Pediatric Nephrology

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Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms' kidney tumours and in vascular remodelling after nephrotoxicity.

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“…Similar findings have also been reported for the binding of bFGF (Nugent and Edelman, ; Zhu et al ., ). The positive effect of aldosterone on tube formation has been demonstrated previously by Minuth's group (Heber et al ., ; Minuth et al ., ); our results seem to be in variance with previous reports, where positive influences were found with these factors and matrices on tubule formation (Kloth et al ., ; Minuth et al ., ; Morimoto et al ., ; Santos et al ., ; Ye et al ., ). The reason for such discrepancy is not immediately clear.…”

Section: Discussionmentioning

confidence: 98%

Controlled tubulogenesis from dispersed ureteric bud-derived cells using a micropatterned gel

Hauser

Nishikawa

Kimura

et al. 2014

J Tissue Eng Regen Med

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Developmental engineering is a potential option for neo-organogenesis of complex organs such as the kidney. The application of this principle requires the ability to construct a tubular structure from dispersed renal progenitor cells with defined size and geometry. In this present study we report the generation of tubular structures from dispersed ureteric bud cells in vitro by using a micropatterned gel. Dispersed CMUB-1 cells, a mouse ureteric bud-derived cell line, or mIMCD cells, a mouse collecting duct-derived cell line, were suspended in collagen I and seeded into an agarose-based micropatterned gel. We found that within 24-36 h of incubation, the cells developed a tubular structure that conformed to the geometry of the micropattern of the gel. The lumen formation of the tubular structure was confirmed by immunohistochemical staining and observed by confocal microscopy. We found that higher concentrations of collagen I negatively influenced the efficiency of tubular formation. Tubule formation in CMUB-1, but not mIMCD, cells was positively influenced by the addition of aldosterone (10, 50 and 200 μg/ml), FGF (50 and 100 μg/ml) and fibronectin (10 and 50 μg/ml) to the growth medium. We further demonstrated the functionality of the generated tubes by in vitro budding, which was induced by growth factors, such as glial cell-derived neurotrophic factor (GDNF) or fibroblast growth factor 7 (FGF7), in the presence of beads soaked with the activin A inhibitor follistatin. Our current study thus demonstrates the possibility of constructing a functional tubular structure from dispersed ureteric bud cells in vitro in a controlled manner.

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Basic fibroblast growth factor is a morphogenic modulator in kidney vessel development

Cited by 9 publications

References 29 publications

Expression of bFGF/FGFR-1 and vascular proliferation related to clinicopathologic features and tumor progress in localized prostate cancer

Expression of bFGF/FGFR-1 and vascular proliferation related to clinicopathologic features and tumor progress in localized prostate cancer

Angiopoietin growth factors and Tie receptor tyrosine kinases in renal vascular development

Controlled tubulogenesis from dispersed ureteric bud-derived cells using a micropatterned gel

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