Basic Fibroblast Growth Factor Affects Neuronal Migration and Differentiation in Normotypic Cell Cultures from the Cochleovestibular Ganglion of the Chick Embryo

Hossain, Waheeda A.; Zhou, Xiuteng; Rutledge, A.; Baier, C.; Morest, D. Kent

doi:10.1006/exnr.1996.0052

Cited by 34 publications

(27 citation statements)

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“…The primary culture system has been characterized previously in chicken embryos (Hossain et al, 1996(Hossain et al, , 1997. In the present study the portion of the murine otocyst fated to form the cochlear ganglion (CG) (Sher, 1971;Li et al, 1976Li et al, , 1978 was dissected from E11 mouse embryos during the period when the ganglion cell precursors were still migrating from the otic epithelium (Figs.…”

Section: Characterization Of the Culturesmentioning

confidence: 99%

“…Although there is considerable experimental evidence implicating FGFs in the early development of the avian inner ear (Represa et al, 1991;Hossain et al, 1996Hossain et al, , 1997Pickles and van Heumen, 1997;Warchol and Kaplan, 1999 ), there is little reported to date on this subject in the mammal. For example, FGF-2 has been shown to stimulate the migration, differentiation and survival of chicken cochleovestibular neurons in vitro (Hossain et al, 1996). At the same time FGF-2 and its receptor expression was found in situ at the appropriate developmental stages.…”

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confidence: 99%

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Fibroblast growth factors (FGF-1, FGF-2) promote migration and neurite growth of mouse cochlear ganglion cells in vitro: Immunohistochemistry and antibody perturbation

Hossain

Morest

2000

J. Neurosci. Res.

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Section: Characterization Of the Culturesmentioning

confidence: 99%

mentioning

confidence: 99%

Fibroblast growth factors (FGF-1, FGF-2) promote migration and neurite growth of mouse cochlear ganglion cells in vitro: Immunohistochemistry and antibody perturbation

Hossain

Morest

2000

J. Neurosci. Res.

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“…In the present study, we examined the distribution of the highaffinity receptor for NT3, that is, TrkC, in the cells and their processes at each critical stage in the morphogenetic sequence of the embryonic CN, including migration, neurite outgrowth, and axonal differentiation. We used a tissue culture model to quantify migration and neurite outgrowth (Hossain et al, 1996(Hossain et al, , 1997(Hossain et al, , 2000(Hossain et al, , 2002, and to test the effects of the relevant factors on these events and on the morphological and molecular markers for axon formation and synaptogenesis.…”

Section: Introductionmentioning

confidence: 99%

Site‐specific interactions of neurotrophin‐3 and fibroblast growth factor (FGF2) in the embryonic development of the mouse cochlear nucleus

2006

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Neurotrophins and FGF2 contribute to formation of the cochlea, but their roles in cochlear nucleus development are unknown. The effects of these factors may differ in the cochlea and cochlear nucleus, which may influence each other's development. It is important to analyze the effects of these factors on cellular structures at well-defined steps in the normal morphogenetic sequence. The present study used immunohistochemistry to localize factors in situ and to test hypotheses about their roles in an in vitro model. Specific antibody staining revealed that TrkC, the NT3 receptor, is present in neural precursors prior to embryonic day E11 until after birth. NT3 appeared in precursor cells during migration (E13-E15) and disappeared at birth. TrkC and NT3 occurred in the same structures, including growing axons, terminals, and their synaptic targets. Thus, NT3 tracks the migration routes and the morphogenetic sequences within a window defined by TrkC. In vitro, the cochlear nucleus anlage was explanted from E11 embryos. Cultures were divided into groups fed with defined medium, with or without FGF2, BDNF, and NT3 supplements, alone or in combinations, for 7 days. When neuroblasts migrated and differentiated, immunostaining was used for locating NT3 and TrkC in the morphogenetic sequence, bromodeoxyuridine for proliferation, and synaptic vesicle protein for synaptogenesis. By time-lapse imaging and quantitative measures, the results support the hypothesis that FGF2 promotes proliferation and migration. NT3 interacts with FGF2 and BDNF to promote neurite outgrowth, fasciculation, and synapse formation. Factors and receptors localize to the structural sites undergoing critical changes.

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“…Moreover, a signal from the endoderm is necessary for Fgf8 to induce mouse and chicken otic development (Ladher et al, 2005). Previous studies in the chicken showed that the ectopic (Adamska et al, 2001), supporting a role for FGF8 in the generation of the CVG (Hossain et al, 1996). Moreover, delaminating neuroblasts are observed at the boundary between the domains of chicken Fgf8 and Otx2 expression (Hidalgo-Sanchez et al, 2000;SanchezCalderon et al, 2004).…”

Section: Diffusible Factors Involved In Inner Ear Neurogenesismentioning

confidence: 89%

“…Fgf2 (bFGF) is expressed in mouse and chicken otic placode and otic vesicle (Vendrell et al, 2000). This FGF augments the migration and differentiation of CVG neurons (Hossain et al, 1996;Zheng et al, 1997;Adamska et al, 2001) and it has been implicated in …”

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confidence: 99%

A network of growth and transcription factors controls neuronal differentation and survival in the developing ear

Sánchez-Calderón¹,

Milo²,

León³

et al. 2007

Int. J. Dev. Biol.

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Inner ear neurons develop from the otic placode and connect hair cells with central neurons in auditory brain stem nuclei. Otic neurogenesis is a developmental process which can be separated into different cellular states that are characterized by a distinct combination of molecular markers. Neurogenesis is highly regulated by a network of extrinsic and intrinsic factors, whose participation in auditory neurogenesis is discussed. Trophic factors include the fibroblast growth factor, neurotrophins and insulin-like peptide families. The expression domains of transcription factor families and their roles in the regulation of intracellular signaling pathways associated with neurogenesis are also discussed. Understanding and defining the key factors and gene networks in the development and function of the inner ear represents an important step towards defeating deafness. KEY WORDS: otic neurogenesis, IGF-I, FGF, inner ear, auditory ganglion, cochlear microarray Otic neurogenesisThe inner ear develops from the otic placode, an ectodermal patch located close to the neural tube that invaginates to form a transitory structure, the otic cup. This cup subsequently closes and pinches off from the ectoderm to generate the otic vesicle ( Figure 1). The otic vesicle is an autonomous structure that contains the information required to generate most of the cell types and structures of the adult inner ear (Bissonnette and Fekete, 1996;Bever et al., 2003; reviewed in Varela-Nieto et al., 2004). The mammalian inner ear is composed of six distinct sensory organs: the three cristae of the semicircular canal, the two maculae of the saccule and utricle and the organ of Corti in the cochlea. The cristae and the maculae are vestibular organs, whereas the organ of Corti is the organ of hearing (reviewed in Moller, 2006). The inner ear cochlear ganglion is formed by bipolar neurons that connect the peripheral sensory receptors or hair cells with central neurons in auditory brain stem nuclei. The correct organogenesis of the inner ear involves a dynamic balance of cell proliferation, differentiation, survival and death, processes that are tightly regulated by a network of extrinsic and intrinsic factors (Frago et al., 2000;Varela-Nieto et al., 2003 andLeon et al., 2004).The process of otic neurogenesis can be separated into different cellular states, each characterized by a distinct combination of molecular markers (summarized in Figure 1A). The first Int. J. Dev. Biol. 51: 557-570 (2007) doi: 10.1387/ijdb.072373hs visible output of otic neurogenesis is the delamination of neural cells (otic neuroblasts) from the otic cup (Fig. 1B). These neuroblasts are committed to generate otic neurons and they populate the cochleo-vestibular ganglion (CVG). This ganglion is generated from a region of the otic vesicle epithelium denominated the prospective neural-sensorial domain and that is defined by the domain in which Ngn1 and Deltal1 are co-expressed at early stages (Adam et al., 1998;Abu-Elmagd et al., 2001). It is believed that both n...

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Basic Fibroblast Growth Factor Affects Neuronal Migration and Differentiation in Normotypic Cell Cultures from the Cochleovestibular Ganglion of the Chick Embryo

Cited by 34 publications

References 0 publications

Fibroblast growth factors (FGF-1, FGF-2) promote migration and neurite growth of mouse cochlear ganglion cells in vitro: Immunohistochemistry and antibody perturbation

Fibroblast growth factors (FGF-1, FGF-2) promote migration and neurite growth of mouse cochlear ganglion cells in vitro: Immunohistochemistry and antibody perturbation

Site‐specific interactions of neurotrophin‐3 and fibroblast growth factor (FGF2) in the embryonic development of the mouse cochlear nucleus

A network of growth and transcription factors controls neuronal differentation and survival in the developing ear

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