Basic FGF as an inducer of anteroposterior neural pattern

Doniach, Tabitha

doi:10.1016/0092-8674(95)90133-7

Cited by 137 publications

(57 citation statements)

References 18 publications

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“…Thus, although FGF alone does not initiate neurogenesis, it may work in concert with neural inducers to induce posterior neural tissue. Factors with this posteriorizing activity have long been sought since the proposition of the two-step neuralpatterning model (Nieuwkoop et al, 1952;Saxén and Toivonen, 1961), for which FGF has been suggested as a top candidate (Doniach, 1995;Sasai and De Robertis, 1997). However, opposite results negating the role of FGF in neural development have been reported by Kroll and Amaya (1996); they found that transgenic embryos with XFD contain well patterned nervous systems despite the severe defect of mesoderm development.…”

Section: Fgf Is Required For Neural Induction and Posteriorizationmentioning

confidence: 82%

“…Additionally another secreted molecule, fibroblast growth factor (FGF), a well recognized mesoderm inducer, has been suggested to be a neural inducer (for review, see Doniach, 1995). First, Okamoto (1993, 1995) found that basic FGF (bFGF) induces dissociated cells of gastrula animal caps (ACs) to differentiate to neurons and melanophores with expression of both anterior and posterior markers.…”

Section: Abstract: Fgf; Bmp; Neurogenesis; Anteroposterior Patterninmentioning

confidence: 99%

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Studies on the Role of Fibroblast Growth Factor Signaling in Neurogenesis Using Conjugated/Aged Animal Caps and Dorsal Ectoderm-Grafted Embryos

Kim

Taira

et al. 1997

J. Neurosci.

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Basic fibroblast growth factor (bFGF) has been shown to induce neural fate in dissociated animal cap (AC) cells or in AC explants cultured in low calcium and magnesium concentrations. However, long-term disclosure of the cap may cause diffusion of the secreted molecule bone morphogenetic protein 4 (BMP-4), a neural inhibitor present in the AC. This may contribute to the subsequent neurogenesis induced by bFGF. Here we used conjugated and aged blastula AC to avoid diffusion of endogenous molecules from the AC. Unlike noggin, bFGF failed to induce neural tissue in this system. However, it enhanced neuralization elicited by a dominant negative BMP receptor (DN-BR) that inhibits the BMP-4 signaling. Posterior neural markers were turned on by bFGF in AC expressing DN-BR or chordin. Blocking the endogenous FGF signal with a dominant negative FGF receptor (XFD) mainly inhibited development of posterior neural tissue in neuralized ACs. These in vitro studies were confirmed in vivo in embryos grafted with XFD-expressing ACs in the place of neuroectoderm. Expression of some regional neural markers was inhibited, although markers for muscle and posterior notochord were still detectable in the grafted embryos, suggesting that XFD specifically affected neurogenesis but not the dorsal mesoderm. The use of these in vitro and in vivo model systems provides new evidence that FGF, although unable to initiate neurogenesis on its own, is required for neural induction as well as for posteriorization.

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Section: Fgf Is Required For Neural Induction and Posteriorizationmentioning

confidence: 82%

Section: Abstract: Fgf; Bmp; Neurogenesis; Anteroposterior Patterninmentioning

confidence: 99%

Studies on the Role of Fibroblast Growth Factor Signaling in Neurogenesis Using Conjugated/Aged Animal Caps and Dorsal Ectoderm-Grafted Embryos

Kim

Taira

et al. 1997

J. Neurosci.

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“…In Xenopus, the ectoderm will pursue an epidermal lineage autonomously, while interaction with dorsal mesoderm is required for the induction of the neural cell fates (Doniach, 1995;Kessler and Melton, 1994). Mesoderm is induced in the marginal zone of the frog blastula through a process that involves a complex interplay between multiple inductive signals (e.g.…”

Section: Regulation Of Xlerk During Mesoderm Induction By Activin Andmentioning

confidence: 99%

Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis

et al. 1997

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“…Although there has been accumulating evidence supporting the 'two step model', the existence of direct trunk neural inducers cannot be ruled out. Two research groups have demonstrated that FGF is a potent neural inducer (Kengaku & Okamoto 1993, 1995Lamb & Harland 1995). Furthermore, in the chicken embryo, it has been shown that FGF can induce posterior neural tissues from epiblasts (Alvarez et al 1998;Storey et al 1998 (Maeda et al 1997;Suzuki et al 1997;Takeda et al 2000.…”

Section: It Has Been Widely Accepted Therefore That the Appearancmentioning

confidence: 99%

Involvement of BMP‐4/msx‐1 and FGF pathways in neural induction in the Xenopus embryo

et al. 2000

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The msx homeodomain protein is a downstream transcription factor of the bone morphogenetic protein (BMP)- results of cell-lineage tracing experiments indicated that the neural cells were differentiated from the animal pole tissue where the excess RNA of either BMP-4 or Xmsx-1 was injected, whereas notochord was differentiated from the organizer mesoderm. Neural tissue differentiated from BMP-4-injected ectodermal cells strongly expressed posterior neural markers, such as hoxB9 and krox20, suggesting that the posterior neural cells differentiated regardless of the existence of the BMP signal. The introduction of a dominant-negative form of the fibroblast growth factor (FGF) receptor (XFD) into the ectodermal cells drastically reduced the expression of pan and posterior neural mark

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Basic FGF as an inducer of anteroposterior neural pattern

Cited by 137 publications

References 18 publications

Studies on the Role of Fibroblast Growth Factor Signaling in Neurogenesis Using Conjugated/Aged Animal Caps and Dorsal Ectoderm-Grafted Embryos

Studies on the Role of Fibroblast Growth Factor Signaling in Neurogenesis Using Conjugated/Aged Animal Caps and Dorsal Ectoderm-Grafted Embryos

Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis

Involvement of BMP‐4/msx‐1 and FGF pathways in neural induction in the Xenopus embryo

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