Basic Characterization of W-pili

Bradley, D. E.; Cohen, Doris R.

doi:10.1099/00221287-97-1-91

Cited by 13 publications

(3 citation statements)

References 23 publications

(18 reference statements)

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“…A prevalent way for bacteria to accomplish adhesion to recipient cells is by the use of external appendages of different size but similar helical structure known as pili (Bradley & Cohen, 1976;Achtman, et al, 1978;Schroder & Lanka, 2005;Thanassi, et al, 2012). At the distal end of these filamentous structures there is a molecule called adhesin (Aly & Baron, 2007), which plays an essential role in cell to cell contact (Anthony, et al, 1994;Dehio, 2004).…”

Section: Pilus Biogenesismentioning

confidence: 99%

Towards an integrated model of bacterial conjugation

et al. 2014

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Bacterial conjugation is one of the main mechanisms for horizontal gene transfer. It constitutes a key element in the dissemination of antibiotic resistance and virulence genes to human pathogenic bacteria. DNA transfer is mediated by a membrane-associated macromolecular machinery called Type IV secretion system (T4SS). T4SSs are involved not only in bacterial conjugation but also in the transport of virulence factors by pathogenic bacteria. Thus, the search for specific inhibitors of different T4SS components opens a novel approach to restrict plasmid dissemination. This review highlights recent biochemical and structural findings that shed new light on the molecular mechanisms of DNA and protein transport by T4SS. Based on these data, a model for pilus biogenesis and substrate transfer in conjugative systems is proposed. This model provides a renewed view of the mechanism that might help to envisage new strategies to curb the threating expansion of antibiotic resistance.

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Section: Pilus Biogenesismentioning

confidence: 99%

Towards an integrated model of bacterial conjugation

et al. 2014

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“…Initial interest in IncW plasmids focused on their small size, wide spectrum of antibiotic resistances, broad host range (Table 1) and their unusual ability to suppress oncogenicity caused by Agrobacterium tumefaciens . Early studies on pSa, R388 and R7K by Bradley & Cohen (1976) determined that IncW pili are rigid filaments, 10–12 nm thick, with an average length of 450 nm. In the genera Escherichia , Salmonella , Shigella and Pseudomonas , W‐pili are present at average frequencies of up to three per cell.…”

Section: Introductionmentioning

confidence: 99%

“…The genetic organization of mobile DNA elements such as plasmids and bacteriophages differs from the chromosomal Bradley & Cohen (1976), Sanderson et al (1981), Gotz et al (1996) Ernstsson et al (2003) organization of their hosts. Plasmid organization, reviewed in detail by Thomas (2000), is based on the confluence and clustering of different survival and propagation functions, arranged in functional modules.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution

Fernández-López¹,

Garcillán-Barcia²,

Revilla³

et al. 2006

FEMS Microbiol Rev

141

150

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Plasmids cannot be understood as mere tools for genetic exchange: they are themselves subject to the forces of evolution. Their genomic and phylogenetic features have been less studied in this respect. Focusing on the IncW incompatibility group, which includes the smallest known conjugative plasmids, we attempt to unveil some common trends in plasmid evolution. The functional modules of IncW genetic backbone are described, with emphasis on their architecture and relationships to other plasmid groups. Some plasmid regions exhibit strong phylogenetic mosaicism, in striking contrast to others of unusual synteny conservation. The presence of genes of unknown function that are widely distributed in plasmid genomes is also emphasized, exposing the existence of ill-defined yet conserved plasmid functions. Conjugation is an essential hallmark of IncW plasmid biology and special attention is given to the organization and evolution of its transfer modules. Genetic exchange between plasmids and their hosts is analysed by following the evolution of the type IV secretion system. Adaptation of the trw conjugative machinery to pathogenicity functions in Bartonella is discussed as an example of how plasmids can change their host modus vivendi. Starting from the phage paradigm, our analysis articulates novel concepts that apply to plasmid evolution.

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Agrobacterium-Mediated Horizontal Gene Transfer

Kado

1998

Genetic Engineering

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Basic Characterization of W-pili

Cited by 13 publications

References 23 publications

Towards an integrated model of bacterial conjugation

Towards an integrated model of bacterial conjugation

Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution

Agrobacterium-Mediated Horizontal Gene Transfer

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