Basic and Advanced Laboratory Techniques in Histopathology and Cytology

Abstract

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Effect of Gallic acid and Myricetin on ovarian cancer models: a possible alternative antitumoral treatment

“…Tissue slices were stained with hematoxylin-eosin (Merck®) or toluidine blue (TOB, Sigma®) and observed by optical microscopy (BX41, Olympus®). Finally, a portion of the tumor was fixed with 2.5% glutaraldehyde for TEM analysis [36]. The remains of animals that were not recovered from the necropsy or preserved in paraformaldehyde, were placed in a yellow polyethylene bag for pathological residues, stored at 4°C and transported to a collection center for biologicalinfectious hazardous residues for subsequent incineration [37].…”

Getting it Right the First Time: Frozen Sections for Diagnosing Necrotizing Soft Tissue Infections

“…This is commonly caused by sampling errors; fat needs lower temperature to freeze compared to most other tissues, but when fat is overfreezed, it shatters more easily. Furthermore, fatty tissues are known to be more difficult to cut which can cause artifacts [ 15 , 16 ]. Due to the instability of subcutaneous fat in frozen sections, subcutaneous fat findings were not included in our criteria.…”

Protocol Improvement for RNA Extraction From Compromised Frozen Specimens Generated in Austere Conditions: A Path Forward to Transcriptomics-Pathology Systems Integration

“…Following necropsy and fixation, tissues received a gross inspection, placed in tissue cassettes (Fisher Scientific, Pittsburgh, PA), and routinely processed. In detail, tissues with 5 micron thickness were paraffin-embedded, sectioned, mounted, stained with hematoxylin and eosin (H&E), followed by the application of a coverslip, and then allowed to dry at room temperature prior to microscopic evaluation (Dey, 2018 ). All tissues were evaluated microscopically by a board-certified veterinary pathologist using representative H&E stained tissue sections.…”