Basement Membrane Helps Maintain Epidermal Hyaluronan Content

Iriyama, Shunsuke; Nishikawa, So; Hosoi, Junichi; Amano, Sadao

doi:10.1016/j.ajpath.2021.03.007

Cited by 7 publications

(7 citation statements)

References 53 publications

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“…Indeed, when the epidermis of elderly people is exposed to sunlight, the BM is somehow damaged by UV exposure, and smaller amounts of HA are observed in exposed epidermis than in unexposed tissue. In addition, the inhibition of metalloproteinases and heparanases, in an in vitro model of full thickness skin equivalent exposed to UV, protects the BM integrity and retains 25% additional HA when compared to HA amount in control epidermis [61]. Consistently, in in vitro rat epidermis model, the setting up of a BM between the epidermis and the collagen matrix was shown to retain approximately 75% HA in the epidermis, whereas 80% of HA was lost in absence of BM [62,63].…”

Section: Degradation Of Hamentioning

confidence: 62%

Epidermal Hyaluronan in Barrier Alteration-Related Disease

Evrard

Rouvroit

Poumay

2021

Cells

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In skin, although the extracellular matrix (ECM) is highly developed in dermis and hypodermis, discrete intercellular spaces between cells of the living epidermal layers are also filled with ECM components. Herein, we review knowledge about structure, localization and role of epidermal hyaluronan (HA), a key ECM molecule. HA is a non-sulfated glycosaminoglycan non-covalently bound to proteins or lipids. Components of the basal lamina maintain some segregation between the epidermis and the underlying dermis, and all epidermal HA is locally synthesized and degraded. Functions of HA in keratinocyte proliferation and differentiation are still controversial. However, through interactions with partners, such as the TSG-6 protein, HA is involved in the formation, organization and stabilization of the epidermal ECM. In addition, epidermal HA is involved in the formation of an efficient epidermal barrier made of cornified keratinocytes. In atopic dermatitis (AD) with profuse alterations of the epidermal barrier, HA is produced in larger amounts by keratinocytes than in normal skin. Epidermal HA inside AD lesional skin is located in enlarged intercellular spaces, likely as the result of disease-related modifications of HA metabolism.

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Section: Degradation Of Hamentioning

confidence: 62%

Epidermal Hyaluronan in Barrier Alteration-Related Disease

Evrard

Rouvroit

Poumay

2021

Cells

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“…MMPs destroy the BM as well as collagen fibers in the ECM of the dermis [ 72 ]. During aging, the levels of proteins that form the BM are decreased [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

The Extracellular Matrix Vitalizer RATM Increased Skin Elasticity by Modulating Mitochondrial Function in Aged Animal Skin

Byun

Batsukh

et al. 2023

Antioxidants

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Oxidative stress-induced cellular senescence and mitochondrial dysfunction result in skin aging by increasing ECM levels-degrading proteins such as MMPs, and decreasing collagen synthesis. MMPs also destroy the basement membrane, which is involved in skin elasticity. The extracellular matrix vitalizer RATM (RA) contains various antioxidants and sodium hyaluronate, which lead to skin rejuvenation. We evaluated whether RA decreases oxidative stress and mitochondrial dysfunction, eventually increasing skin elasticity in aged animals. Oxidative stress was assessed by assaying NADPH oxidase activity, which is involved in ROS generation, and the expression of SOD, which removes ROS. NADPH oxidase activity was increased in aged skin and decreased by RA injection. SOD expression was decreased in aged skin and increased by RA injection. Damage to mitochondrial DNA and mitochondrial fusion markers was increased in aged skin and decreased by RA. The levels of mitochondrial biogenesis markers and fission markers were decreased in aged skin and increased by RA. The levels of NF-κB/AP-1 and MMP1/2/3/9 were increased in aged skin and decreased by RA. The levels of TGF-β, CTGF, and collagen I/III were decreased in aged skin and increased by RA. The expression of laminin and nidogen and basement membrane density were decreased in aged skin and increased by RA. RA increased collagen fiber accumulation and elasticity in aged skin. In conclusion, RA improves skin rejuvenation by decreasing oxidative stress and mitochondrial dysfunction in aged skin.

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“…A large amount of HA diffuses into the subepidermal compartment, suggesting a role for TSG-6 in maintaining HA inside the tissue. HA leakage from the epidermis was already reported in skin equivalent models, and attributed to an improper organization of the basement membrane (BM) (Iriyama et al, 2021;Tammi et al, 2000). Nonetheless, Rosdy et al (1993) had illustrated typical basal lamina elements in RHE.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 90%