Basella alba L.: In Vitro Culture and the Production of Betalains

Cyunel, E.

doi:10.1007/978-3-642-73617-9_3

Cited by 11 publications

(9 citation statements)

References 39 publications

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“…The method of multiplication of shoots by repetitive transfer of mother explants has been reported in many plants (Sanchez et al 1997;Panwar et al 2012). The number of shoots produced in this study was significantly greater than those achieved in the earlier reports in B. alba (Cyunel 1989;Guo and Bin 2001) and B. rubra (Pumchaosuan and Wongroung 2009). Guo and Bin (2001) were able to regenerate maximum four shoots/explant from the callus induced from hypocotyl explants on MS medium containing BA (2.0 mg/L) ?…”

Section: Multiplication Of Shoots and Maintenance Of Culturescontrasting

confidence: 75%

“…They observed callus from the explant surface, when MS medium was fortified with 6-BA alone or with NAA or 2,4-D. No callus was reported with the cytokinins as well as auxins during this study at any stage of the cultures. Cyunel (1989) established cultures for the production of pigments using B. alba cells. Effectiveness of BAP over Kin for shoot initiation from the buds has been reported in number of plant species like Ceropegia bulbosa (Phulwaria et al 2013), Leptadenia reticulata , Turnera ulmifolia and Morinda coreia (Shekhawat et al , 2015a.…”

Section: Selection Of Explants and Establishment Of Culturesmentioning

confidence: 99%

“…Basella alba is now cultivated for its industrial purposes and naturally propagated by the stem cuttings (Cyunel 1989), this method causes the carry-over of disease-causing pathogens from one generation to the next (Sankar et al 2011). The propagation through seeds has some limitations due some special requirements in germination of seeds and proper flowering in this plant (Larkcom 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Plant tissue culture methods can be used for rapid multiplication of improved varieties and to produce disease free and quality planting materials (Kartha and Gamborg 1975). Some reports are available on the tissue culture and anthocyanin production using B. alba cells (Cyunel 1989;Guo and Bin 2001). However, efficient in vitro propagation protocol for this plant species is largely deficit in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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The optimum concentrations of the plant hormones for in vitro regeneration and subsequent effect of auxins on rooting (in vitro and ex vitro) of shoots of L. have been investigated in present study. Nodal shoot segments were used as explants to initiate the cultures. The bud breaking from explants was observed within 1 week of incubation on agar gelled Murashige and Skoog's (MS) medium. Multiple axillary shoots (7.30 ± 0.56 shoots per explant) were induced on MS medium supplemented with 2.0 mg/L 6-benzylaminopurine (BAP). The shoots were multiplied (maximum 17.10 ± 0.44 shoots per explant) on the same medium fortified with 0.5 mg/L each of BAP and Kin (Kinetin) +0.1 mg/L IAA. These shoots were excised and rooted in vitro (10.73 ± 0.92 roots per shoot) on half-strength MS medium augmented with 2.0 mg/L indole-3 butyric acid (IBA). Hundred percentage success rates have been achieved by ex vitro rooting of the in vitro regenerated shoots with IBA at 300 mg/L. The in vitro and ex vitro rooted shoots were acclimatized in greenhouse and subsequently transferred to the natural field conditions where 100 % survival rate was reported. The ex vitro rooting method was found more advantageous than in vitro rooting in terms of time, energy and survival percentage of. A comparative foliar micromorphological study of was conducted to understand the micromorphological changes in plants while shifting from in vitro to the in vivo conditions in terms of variations in stomatal index, venation pattern and vein density, and the arrangement of crystals. The study could help in understanding the response of in vitro raised plants towards in vivo environment.

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Section: Multiplication Of Shoots and Maintenance Of Culturescontrasting

confidence: 75%

Section: Selection Of Explants and Establishment Of Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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show abstract

“…Earlier betalains were generally reviewed in plant cell cultures in general (see Böhm and Rink 1988;Cyunel 1989), however, the following is an up-to-date report in P. grandifiora cell cultures.…”

Section: Betalains In P Grandiflora Cell Culturesmentioning

confidence: 97%