Based on Plasma Metabonomics and Network Pharmacology Exploring the Therapeutic Mechanism of Gynura procumbens on Type 2 Diabetes

Abstract

Gynura procumbens (GP) is a perennial herbal medicine and food homologous plant, which has been reported to have a good hypoglycemic effect. However, its active components and underlying mechanism of action are not clear. Here, we aimed to confirm the effects of GP on type 2 diabetes (T2DM) from several different aspects. We used UPLC/Q-TOF MS to analyze the metabolic patterns, which included blood samples of clinical subjects and db/db mice to screen for serum metabolic markers and metabolic pathways. We also used network pharmacology to study GP targets in the treatment of T2DM. Data from endogenous metabolites in plasma showed that two common pathways, including glycerol phosphate metabolism and retinol metabolism, were identified in plasma samples of the groups. Finally, Western blot analysis was used to verify the expression of proteins in the PI3K/AKT and AGE–RAGE signaling pathways. The protein expression of AKT, eNOS, iNS, and MAPK was significantly upregulated, and the expression of caspase-8 and caspase-3 was significantly downregulated. Thus, our findings indicated that GP could alleviate insulin resistance by regulating biometabolic markers and key proteins in the PI3K/AKT and AGE–RAGE signaling pathways to treat T2DM.

Uji Karakteristik Sediaan Salep Ekstrak Etanol Daun Sambung Nyawa (Gynura Procumbens (Lour.) Merr.)

“…Pada penelitian (Widyawati, 2007) menemukan bahwa Gynura procumbens (Lour.) Merr, bermanfaat sebagai antikanker (Jermnak et al, 2022); terapi untuk diabetes (Sutthammikorn et al, 2021); antiinfeksi (Ahmad Nazri et al, 2021); antioksidan (Sabarany lado et al, 2021); antihipertensi (Shahlehi & Petalcorin, 2021); diabetes (Guo et al, 2021); antiinflamasi (Novia et al, 2022); cedera hati (Lee & Hyun, 2022); antibakteri (Ashraf et al, 2020). Selain itu, penelitian sebelumnya menemukan bahwa ramuan ini digunakan sebagai pereda nyeri, pengobatan luka bakar dan spesialis mitigasi.…”

“…Selain itu, penelitian sebelumnya menemukan bahwa ramuan ini digunakan sebagai pereda nyeri, pengobatan luka bakar dan spesialis mitigasi. Pada pengobatan luka bakar dalam masyarakat digunakan dengan cara daun sambung nyawa dihaluskan dahulu kemudian letakkan daun yang telah dihaluskan pada bagian luka bakar (Guanabara et al, 2020).…”

“…Berdasarkan banyaknya khasiat dari daun sambung nyawa, maka diyakini bahwa daun sambung nyawa mengandung senyawa metabolit sekunder yang sangat bermanfaat bagi kesehatan (Guanabara et al, 2020). Daun sambung nyawa yang diekstrak memiliki senyawa kimia yaitu flavonoid, alkaloid, saponin, glikosida, tannin (Hidayah, 2016).…”

Protective effect of Xixin–Ganjiang herb pair for warming the lungs to dissolve phlegm in chronic obstructive pulmonary disease rats based on integrated network pharmacology and metabolomics

“…Metabolomics is the study of metabolic pathways and measurement of unique biomolecules in living systems (Ho et al, 2013). Network pharmacology technology has been successfully applied to the study of active ingredients and potential targets in Chinese medicine as a virtual computer contract method (Guo et al, 2021). In the current study, serum metabolomics and network pharmacology were used to analyze the therapeutic effects and pathways of XGHP in rats with COPD, the syndrome of cold phlegm obstruction of lungs , and Western blot analysis was used to verify the expression of target proteins in lung tissues.…”

Experimental study of the hepatoprotective effect of the aqueous extract of Jinura Procumbens leaves on the model of fructose-induced non-alcoholic liver steatosis

“…Джинура Прокумбенс носит официальное международное название Gynura Procumbens, растение из сложноцветущего семейства астровых содержит клетчатку, растительный белок, каратиноиды, флавоноиды, алкалоиды и эфирные масла, оказывающие лечебно-профилактические эффекты, прежде всего, за счет расщепления до мономерных соединений. В ряде исследований было найдено положительное влияние β-ситостеринов при метаболическом синдроме у пациентов с ожирением [9][10][11][12][13]. По данным литературы экстракт из стеблей ДП снижает уровень ферментов и накопление липидов в печеночной ткани [14].…”