Base‐Sequence‐Independent Efficient Redox Switching of Self‐Assembled DNA Nanocages

Wang, Bang; Song, Lei; Jin, Bang Kun; Deng, Ning; Wu, Xiaojing; He, Jianbo; Deng, Zhaoxiang; Li, Yulin

doi:10.1002/cbic.201900253

Cited by 4 publications

(7 citation statements)

References 46 publications

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“…To allow the DNA crystal to respond to the redox potential, we introduced cystamine (Cyst) into the buffer. [31][32][33] In Cyst, two amino groups are joined together by a redox-sensitive disulfide bond. At neutral pH, Cyst exists as Cyst 2+ with both amino groups protonated.…”

Section: Dna Crystals Respond To Redox Reagentsmentioning

confidence: 99%

Powering ≈50 µm Motion by a Molecular Event in DNA Crystals

Zheng

Zhang

et al. 2022

Advanced Materials

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A major challenge in material design is to couple nanoscale molecular and supramolecular events into desired chemical, physical, and mechanical properties at the macroscopic scale. Here, a novel self‐assembled DNA crystal actuator is reported, which has reversible, directional expansion and contraction for over 50 μm in response to versatile stimuli, including temperature, ionic strength, pH, and redox potential. The macroscopic actuation is powered by cooperative dissociation or cohesion of thousands of DNA sticky ends at the designed crystal contacts. The increase in crystal porosity and cavity in the expanded state dramatically enhances the crystal capability to accommodate/encapsulate nanoparticles/proteins, while the contraction enables a “sponge squeezing” motion for releasing nanoparticles. This crystal actuator is envisioned to be useful for a wide range of applications, including powering self‐propelled robotics, sensing subtle environmental changes, constructing functional hybrid materials, and working in drug controlled‐release systems.

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Section: Dna Crystals Respond To Redox Reagentsmentioning

confidence: 99%

Powering ≈50 µm Motion by a Molecular Event in DNA Crystals

Zheng

Zhang

et al. 2022

Advanced Materials

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“…On this basis, DNA nanostructures that could be formed and deformed with kinetic redox control via disulfide bonds were designed . Another switchable DNA self-assembly was created via cystamine and cysteamine interconversion . The assembly and disassembly processes were controlled by the addition of peroxide and GSH, respectively, which could be used as a stimulus-responsive cargo delivery system.…”

Section: Nucleic Acid Carriers With Disulfide-sensitive Functionalitiesmentioning

confidence: 99%

“…209 Another switchable DNA self-assembly was created via cystamine and cysteamine interconversion. 210 The assembly and disassembly processes were controlled by the addition of peroxide and GSH, respectively, which could be used as a stimulus-responsive cargo delivery system. Creation of a disulfide-containing molecular sticker (DSMS) on a DNA nanostructure was reported to aid in cellular uptake.…”

mentioning

confidence: 99%

Disulfide Bridging Strategies in Viral and Nonviral Platforms for Nucleic Acid Delivery

Dutta¹,

Das²,

Medeiros³

et al. 2021

Biochemistry

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Self-assembled nanostructures that are sensitive to environmental stimuli are promising nanomaterials for drug delivery. In this class, disulfide-containing redox-sensitive strategies have gained enormous attention because of their wide applicability and simplicity of nanoparticle design. In the context of nucleic acid delivery, numerous disulfide-based materials have been designed by relying on covalent or noncovalent interactions. In this review, we highlight major advances in the design of disulfide-containing materials for nucleic acid encapsulation, including covalent nucleic acid conjugates, viral vectors or virus-like particles, dendrimers, peptides, polymers, lipids, hydrogels, inorganic nanoparticles, and nucleic acid nanostructures. Our discussion will focus on the context of the design of materials and their impact on addressing the current shortcomings in the intracellular delivery of nucleic acids.

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“…Subsequent disassembly can be achieved via glutathione (GSH)‐mediated reduction of the resulting disulfide bond. Figure adapted from Wang et al (Wang et al, 2019). (d) Azobenzene UV‐triggered trans to trans cis isomerization (left) and a schematic of the light‐droven opening and closing of azobenzene‐incorporated DNA nano‐tweezers (right).…”

Section: Functional Applications Of Dna Nanostructuresmentioning

confidence: 99%

“…Considering the ubiquitous existence of reductive or oxidative environments in physiological contexts, the development of redox-sensitive DNs would provide many therapeutically relevant applications. A recent, generalizable example of such a system was demonstrated by Wang et al (Wang et al, 2019). DNA tetrahedra were electrostatically assembled with the aid of positively charged cystamine, which features a redox-active disulfide bond in the center of its chemical structure.…”

Section: Oxidation Statementioning

confidence: 99%

Functionalizing DNA nanostructures for therapeutic applications

Henry

Stephanopoulos

2021

WIREs Nanomed Nanobiotechnol

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Recent advances in nanotechnology have enabled rapid progress in many areas of biomedical research, including drug delivery, targeted therapies, imaging, and sensing. The emerging field of DNA nanotechnology, in which oligonucleotides are designed to self‐assemble into programmable 2D and 3D nanostructures, offers great promise for further advancements in biomedicine. DNA nanostructures present highly addressable and functionally diverse platforms for biological applications due to their ease of construction, controllable architecture and size/shape, and multiple avenues for chemical modification. Both supramolecular and covalent modification with small molecules and polymers have been shown to expand or enhance the functions of DNA nanostructures in biological contexts. These alterations include the addition of small molecule, protein, or nucleic acid moieties that enable structural stability under physiological conditions, more efficient cellular uptake and targeting, delivery of various molecular cargos, stimulus‐responsive behaviors, or modulation of a host immune response. Herein, various types of DNA nanostructure modifications and their functional consequences are examined, followed by a brief discussion of the future opportunities for functionalized DNA nanostructures as well as the barriers that must be overcome before their translational use. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Therapeutic Approaches and Drug Discovery > Emerging Technologies Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures

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Base‐Sequence‐Independent Efficient Redox Switching of Self‐Assembled DNA Nanocages

Cited by 4 publications

References 46 publications

Powering ≈50 µm Motion by a Molecular Event in DNA Crystals

Powering ≈50 µm Motion by a Molecular Event in DNA Crystals

Disulfide Bridging Strategies in Viral and Nonviral Platforms for Nucleic Acid Delivery

Functionalizing DNA nanostructures for therapeutic applications

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