2013
DOI: 10.1021/jp406993m
|View full text |Cite
|
Sign up to set email alerts
|

Base Pair Sensitivity and Enhanced ON/OFF Ratios of DNA-Binding: Donor–Acceptor–Donor Fluorophores

Abstract: The photophysical properties of two recently reported live cell compatible, DNA-binding dyes, 4,6-bis(4-(4-methylpiperazin-1-yl)phenyl)pyrimidin-2-ol, 1, and [1,3-bis[4-(4-methylpiperazin-1-yl)phenyl]-1,3-propandioato-κO, κO']difluoroboron, 2, are characterized. Both dyes are quenched in aqueous solutions, while binding to sequences containing only AT pairs enhances the emission. Binding of the dyes to sequences containing only GC pairs does not produce a significant emission enhancement, and for sequences con… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
5

Citation Types

1
16
1

Year Published

2016
2016
2024
2024

Publication Types

Select...
4
1

Relationship

0
5

Authors

Journals

citations
Cited by 17 publications
(18 citation statements)
references
References 26 publications
(51 reference statements)
1
16
1
Order By: Relevance
“…We exploited the fluorescence anisotropy of boron nanoparticle luminescence to investigate the organization of fluorophores within the nanoparticles, the orientation of the absorbing and emitting dipoles, and the importance of energy migration within the nanoparticles. Understanding changes in fluorescence anisotropy based on dye concentrations and orientation in a well‐understood environment such as polylactide, can inform and better predict results in complex biological systems, such as cell membranes, proteins, or DNA …”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…We exploited the fluorescence anisotropy of boron nanoparticle luminescence to investigate the organization of fluorophores within the nanoparticles, the orientation of the absorbing and emitting dipoles, and the importance of energy migration within the nanoparticles. Understanding changes in fluorescence anisotropy based on dye concentrations and orientation in a well‐understood environment such as polylactide, can inform and better predict results in complex biological systems, such as cell membranes, proteins, or DNA …”
Section: Introductionmentioning
confidence: 99%
“…Understanding changes in fluorescence anisotropy based on dye concentrations and orientation in a well-understood environment such as polylactide, can inform and better predict results in complex biological systems, such as cell membranes, [23,24] proteins, [25] or DNA. [26]…”
Section: Introductionmentioning
confidence: 99%
“…By installing different substituted conjugative groups, large molar emission ratios and high quantum yields could be realized for the BF 2 complexes of β‐diketonates, which could serve as photochemical reagents, two‐photon materials, and near‐IR probes 2–5. Recently, various BF 2 complexes of β‐diketonates,2–5 β‐keto esters,6 and β‐keto amides7 have been synthesized and extensively studied. However, an investigation of malonamide‐type BF 2 complexes is very rare 8…”
Section: Introductionmentioning
confidence: 99%
“…Difluoroboron (BF 2 ) complexes of N,N‐ and N,O‐based ligands are generally highly fluorescent substances in the solution, film, and solid state 1. Recently, difluoroboron complexes of β‐dicarbonyl compounds have been widely investigated 25. β‐Diketonates, possessing two carbonyls, can easily react with boron trifluoride etherate (BF 3 ⋅OEt 2 ) to form six‐membered heterocyclic difluoroboron complexes.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation