Basal stomatal aperture is regulated by GA-DELLAs in Arabidopsis

Sukiran, Nur Afiqah; Steel, Patrick G.; Knight, Marc R.

doi:10.1016/j.jplph.2020.153182

Cited by 9 publications

(7 citation statements)

References 22 publications

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“…Steady‐state stomatal aperture is crucial for plant adaptation to abiotic stress (Cui et al ., 2015; Murata et al ., 2015; Sukiran et al ., 2020) and, therefore, the positive regulation of OsWRKY45 expression by the high basal shoot SA content could play a role in the plant tolerance to abiotic stress in rice. To test this, we first assessed the responses of WT and the Osaim1 to salt and PEG treatments.…”

Section: Resultsmentioning

confidence: 99%

“…Although many studies have analyzed the effect of SA on abiotic stresses in plants, its role and molecular mechanism in abiotic New Phytologist (2023) 238: 1420-1430 www.newphytologist.com Ó 2023 The Authors New Phytologist Ó 2023 New Phytologist Foundation stress tolerance in rice are still poorly understood. Steady-state stomatal aperture is a key component in plant adaption to abiotic stress (Cui et al, 2015;Murata et al, 2015;Sukiran et al, 2020). Therefore, the high basal SA level in rice shoots may maintain the steady-state stomatal aperture to increase the abiotic stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

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AIM1‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice

Zhao

Wang

et al. 2023

New Phytologist

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The basal levels of salicylic acid (SA) vary dramatically among plant species. In the shoot, for example, rice contains almost 100 times higher SA levels than Arabidopsis. Despite its high basal levels, neither the biosynthetic pathway nor the biological functions of SA are well understood in rice.Combining with metabolite analysis, physiological, and genetic approaches, we found that the synthesis of basal SA in rice shoot is dependent on OsAIM1, which encodes a betaoxidation enzyme in the phenylalanine ammonia-lyase (PAL) pathway.Compromised SA accumulation in the Osaim1 mutant led to a lower shoot temperature than wild-type plants. However, this shoot temperature defect resulted from increased transpiration due to elevated steady-state stomatal aperture in the mutant. Furthermore, the high basal SA level is required for sustained expression of OsWRKY45 to modulate the steadystate stomatal aperture and shoot temperature in rice.Taken together, these results provide the direct genetic evidence for the critical role of the PAL pathway in the biosynthesis of high basal level SA in rice, which plays an important role in the regulation of steady-state stomatal aperture to promote fitness under stress conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

AIM1‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice

Zhao

Wang

et al. 2023

New Phytologist

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“…Steady-state stomatal aperture is crucial for plant adaptation to abiotic stress (27, 29, 30) and, therefore, the positive regulation of OsWRKY45 expression by the high basal shoot SA content could play a role in the plant tolerance to abiotic stress in rice. To test this, we first assessed the responses of WT and the Osaim1 to salt and polyethylene glycol (PEG) treatments.…”

Section: Resultsmentioning

confidence: 99%

“…Although many studies have analyzed the effect of SA on abiotic stresses in plants, its role and molecular mechanism in abiotic stress tolerance in rice is still poorly understood. Steady-state stomatal aperture is a key component in plant adaption to abiotic stress (27, 29, 30). Therefore, the high basal SA level in rice shoot may maintain the steady-state stomatal aperture to increase the abiotic stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

AIM1-dependent high basal SA accumulation modulates stomatal aperture in rice

Zhao

Wang

et al. 2022

Preprint

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The basal levels of salicylic acid (SA), an important plant hormone, vary dramatically among plant species. In the shoot, for example, the monocot plant rice contains almost 100 times higher SA levels than the dicot model plant Arabidopsis. Despite its high basal levels, neither the biosynthetic pathway nor the biological functions of SA is well understood in rice. Here, we report that the synthesis of basal SA in rice shoot is not altered in the mutant for the ISOCHORISMATE SYNTHASE (ICS) gene, but drastically reduced in the mutant for OsAIM1, which encodes a beta-oxidation enzyme in the phenylalanine ammonia-lyase (PAL) pathway. Analogous to its role in thermogenesis, compromised SA accumulation in the Osaim1 mutant led to a lower shoot temperature than wild-type plants. However, this shoot temperature defect was resulted from increased transpiration due to elevated steady-state stomatal aperture in the mutant. Furthermore, the high basal shoot SA level is required for sustained expression of WRKY45 to modulate the steady-state stomatal aperture and shoot temperature in rice. Taken together, these results provide the direct genetic evidence for the critical role of the PAL pathway in the biosynthesis of high levels of basal SA, which play an important role in the regulation of steady-state stomatal aperture to promote fitness under both normal and stress conditions.

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“…Among them, ABA is a phytohormone that affects stomatal closure to maintain intracellular water balance and is one of the most important phytohormones in plant drought stress response ( Morgil et al, 2019 ). DELLA proteins act as positive regulators of stomatal closure in tomato, in addition their action is enhanced by the hormone ABA, which is itself important in mediating drought stress tolerance ( Sukiran, Steel & Knight, 2020 ). Therefore, illustrating ABA-mediated metabolic pathways may adapt to drought stress by influencing stomatal mobility in I. bungeana .…”

Section: Discussionmentioning

confidence: 99%

Physiological response and transcriptome analyses of leguminous Indigofera bungeana Walp. to drought stress

Ma,

Hu,

Zhang

et al. 2023

PeerJ

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Objective Indigofera bungeana is a shrub with high quality protein that has been widely utilized for forage grass in the semi-arid regions of China. This study aimed to enrich the currently available knowledge and clarify the detailed drought stress regulatory mechanisms in I. bungeana, and provide a theoretical foundation for the cultivation and resistance breeding of forage crops. Methods This study evaluates the response mechanism to drought stress by exploiting multiple parameters and transcriptomic analyses of a 1-year-old seedlings of I. bungeana in a pot experiment. Results Drought stress significantly caused physiological changes in I. bungeana. The antioxidant enzyme activities and osmoregulation substance content of I. bungeana showed an increase under drought. Moreover, 3,978 and 6,923 differentially expressed genes were approved by transcriptome in leaves and roots. The transcription factors, hormone signal transduction, carbohydrate metabolism of regulatory network were observed to have increased. In both tissues, genes related to plant hormone signaling transduction pathway might play a more pivotal role in drought tolerance. Transcription factors families like basic helix-loop-helix (bHLH), vian myeloblastosis viral oncogene homolog (MYB), basic leucine zipper (bZIP) and the metabolic pathway related-genes like serine/threonine-phosphatase 2C (PP2C), SNF1-related protein kinase 2 (SnRK2), indole-3-acetic acid (IAA), auxin (AUX28), small auxin up-regulated rna (SAUR), sucrose synthase (SUS), sucrosecarriers (SUC) were highlighted for future research about drought stress resistance in Indigofera bungeana. Conclusion Our study posited I. bungeana mainly participate in various physiological and metabolic activities to response severe drought stress, by regulating the expression of the related genes in hormone signal transduction. These findings, which may be valuable for drought resistance breeding, and to clarify the drought stress regulatory mechanisms of I. bungeana and other plants.

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Basal stomatal aperture is regulated by GA-DELLAs in Arabidopsis

Cited by 9 publications

References 22 publications

AIM1‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice

AIM1‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice

AIM1-dependent high basal SA accumulation modulates stomatal aperture in rice

Physiological response and transcriptome analyses of leguminous Indigofera bungeana Walp. to drought stress

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