Basal serum nitric oxide levels in patients with type 2 diabetes mellitus and different stages of retinopathy

Özden, Serap; Tathpinar, Sinan; Bigçer, Nilgün; Yaylalı, Volkan; Yıldırım, Cem; Özbay, Davut; Güner, Gül

doi:10.1016/s0008-4182(03)80051-0

Cited by 38 publications

(29 citation statements)

References 14 publications

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“…In a similar way, increased NOS activity has been reported in the sciatic nerves of diabetic rats (35). Elevated levels of serum NO have been found in patients with type II diabetic retinopathy compared with non-diabetic controls, and a relationship has been observed between NO and diabetic retinopathy severity (36). An association has been reported between the existence of neuropathy in diabetic patients and endothelial NOS gene polymorphism (37).…”

Section: Discussionsupporting

confidence: 62%

Diyabetik nöropatili hastalarında serum asimetrik dimetilarjinin ve nitrik oksit düzeyleri

...

2012

npa

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Re cei ved/Ge liş ta ri hi: 21.07.2011 Ac cep ted/Ka bul ta ri hi: 16.12.2011 © Arc hi ves of Neu ropsy chi atry, pub lis hed by Ga le nos Pub lis hing / © Nö rop si ki yat ri Ar şi vi Der gi si, Ga le nos Ya yı ne vi ta ra f›n dan ba s›l m›fl t›r. ABS TRACTObjective: Asymmetric dimethylarginine (ADMA) is a novel marker of endothelial dysfunction. ADMA is an endothelial nitric oxide synthase inhibitor. Due to scarcity of studies on the role of ADMA in pathogenesis of diabetic neuropathy, it remains to be determined whether blood ADMA and nitric oxide (NO) levels are associated with diabetic neuropathy. In this case control study, we aimed to evaluate the relationship between serum NO and ADMA levels among diabetic neuropathy cases Methods: 48 patients with diabetic neuropathy and 34 healthy subjects were included in the study. All type 2 diabetic patients underwent electrophysiological and neurological examination. NO levels were determined using the Griess method. Serum ADMA levels were determined by using the ELISA method. Results: The serum ADMA levels in diabetic neuropathy patients were found to be statistically significantly lower than those of the control group (p=0.005). However, a statistically significant increase was observed in serum NO levels in patients with diabetic neuropathy compared with those in the control group (p = 0.009). A negative correlation was found between NO levels and ADMA levels (p=0.041, r=-0.23). Discussion: Changes in NO and ADMA levels in the blood of diabetic neuropathy patients may indicate oxidative stress and endothelial dysfunction in the pathogenesis of diabetic neuropathy. (Arc hi ves of Neu ropsy chi atry 2012; 49: 183-187)

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Section: Discussionsupporting

confidence: 62%

Diyabetik nöropatili hastalarında serum asimetrik dimetilarjinin ve nitrik oksit düzeyleri

...

2012

npa

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“…Izumi et al found an increase in plasma levels of nitric oxide in patients with diabetes with or without DR in comparison to patients with no diabetes mellitus. Ozden et al documented a significant increase in the serum levels of nitric oxide with increased severity of diabetic retinopathy [33]. Attenuation of oxidative and nitrative stress by anti-oxidant treatment in diabetic animals further corroborate these findings [34, 35].…”

Section: Discussionmentioning

confidence: 74%

Correlation of biomarkers thiobarbituric acid reactive substance, nitric oxide and central subfield and cube average thickness in diabetic retinopathy: a cross-sectional study

et al. 2016

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BackgroundTo evaluate the role of thiobarbituric acid reactive substance (TBARS) and nitric oxide (NO) as biochemical biomarkers and central subfield (CST) and cube average thickness (CAT) as biomarkers for medical imaging in diabetic retinopathy.MethodsForty consecutive cases of diabetic retinopathy and 20 healthy controls were included. Cases were divided into two groups: non proliferative diabetic retinopathy (n = 20) and proliferative diabetic retinopathy (n = 20) according to ETDRS classification. LogMAR visual acuity was documented. Plasma levels of TBARS, NO and glycated hemoglobin (HbA1c) were measured using standard protocol. CST and CAT were analyzed on spectral domain optical coherence tomography. Data was analyzed statistically.ResultsIncreased severity of diabetic retinopathy was associated with an increase in plasma levels of TBARS (F = 10.92; p < 0.001), NO (F = 21.8; p < 0.001) and HbA1c (F = 5.87; p = 0.001). Increase in CST (F = 61.51; p < 0.001) and CAT (F = 60.84; p < 0.001) was also found to be associated with increased severity of diabetic retinopathy. Pearson’s correlation analysis revealed a positive correlation of TBARS with CST (r = 0.29; p = 0.038) and CAT (r = 0.31; p = 0.04). A positive correlation of NO with CST (r = 0.27; p = 0.03) and CAT (r = 0.7; p = 0.001) was also observed. On univariate analysis with logMAR visual acuity as dependent variable, a significant increase in visual acuity was observed with increase in independent variables TBARS (B = 0.22; p = 0.004), NO (B = 0.006; p < 0.001), CST (B = 0.005; p < 0.001) and CAT (B = 0.005; p < 0.001). On multivariate linear regression analysis with logMAR visual acuity as dependent variable and adjusting for other factors like duration of diabetes and HbA1c, it was observed that increase in independent variables TBARS (B = 0.07), NO (B = 0.001) and CST (B = 0.004) independently predict increase in logMAR visual acuity (p < 0.001).ConclusionThiobarbituric acid reactive substance and nitric oxide serve as potential biochemical markers whereas central subfield and cube average thickness serve as potential biomarkers for medical imaging for severity of diabetic retinopathy. In a clinical retinal setting, CAT and CST will help in early recognition of increase in severity of diabetic retinopathy.

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“…The patients with type 2 diabetes had significantly higher levels of serum NOx than the non-diabetics. [20] A Turkish study on micro- and normo-albuminuric type 2 diabetics and healthy controls found that serum and urine NO levels were higher in both micro-albuminurics and normo-albuminurics than controls in early diabetes. [21] A Japanese study showed that plasma NO x levels were significantly higher in diabetics than in controls, when measured by high-performance liquid chromatography with the Griess method.…”

Section: Discussionmentioning

confidence: 99%

Serum nitric oxide status in patients with type 2 diabetes mellitus in Sikkim

Ghosh

Sherpa

Bhutia

et al. 2011

Int J App Basic Med Res

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Background:Serum nitric oxide (NO) has emerged as a fundamental signal associated with the endothelial dysfunction in type 2 diabetes.Aims:To compare serum nitric oxide level among type 2 diabetic patients along with other biochemical parameters and to compare it with that of normal population in Sikkim.Settings and Design:This prospective study was carried out in the Biochemistry Department in a tertiary care teaching hospital in Sikkim on 50 type 2 diabetics compared to 100 non-diabetics.Materials and Methods:The un-hemolyzed blood sample was collected for estimation of biochemical parameters. Griess reaction was used for indirect assay of stable decomposition products in serum(serum nitrite and nitrate levels) as an index of NO generation. The comparison of different parameters between cases and control was done by using Student's t-test.Results:There was significant difference when age- and sex-matched cases and controls were compared in regard to waist circumference and body mass index. The values of fasting and postprandial serum glucose, and lipid profile between study group and control group differed significantly. The mean serum level of NO in the study and control group was 43.83 ± 11.3 μmoles/L and 58.85 ± 12.8 μmoles/L respectively, and this difference was statistically significant.Conclusion:To sum up, serum NO was observed significantly low in diabetic participants as compared to control, along with difference in other biochemical parameters.

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Basal serum nitric oxide levels in patients with type 2 diabetes mellitus and different stages of retinopathy

Cited by 38 publications

References 14 publications

Diyabetik nöropatili hastalarında serum asimetrik dimetilarjinin ve nitrik oksit düzeyleri

Diyabetik nöropatili hastalarında serum asimetrik dimetilarjinin ve nitrik oksit düzeyleri

Correlation of biomarkers thiobarbituric acid reactive substance, nitric oxide and central subfield and cube average thickness in diabetic retinopathy: a cross-sectional study

Serum nitric oxide status in patients with type 2 diabetes mellitus in Sikkim

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