Basal prostaglandin synthesis by the isolated perfused rat kidney

Liu, K.L.; Aissa, H.Hadi; Laréal, M. C.; Benzoni, Daniel; Sassard, J; Zech, P

doi:10.1016/0952-3278(90)90004-5

Cited by 5 publications

(11 citation statements)

References 23 publications

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“…blockade significantly reduced the vascular responses to NE and enhanced the GFR and the pressurenatriuresis. We used the isolated perfused kidney because we have demonstrated it to be a valuable preparation for the study of prostanoid synthesis 16 that allows control of factors that may influence renal function. In addition, the use of an open perfusion circuit allowed us to avoid the recirculation of renally produced prostanoids and to separate the administration of NE and PHE by washout periods so as to prevent tachyphylaxis.…”

Section: Discussionmentioning

confidence: 99%

“…Such a difference may be explained by the higher density of renal a,-adrenergic receptors exhibited by LH rats compared with LN and LL rats. 32 The urinary excretion of prostanoids, which has previously been demonstrated to be a reliable index of their renal synthesis, 16 did not differ among kidneys of the three strains during the baseline period. Such a finding demonstrates that the increased TxA 2 renal synthesis observed in vivo in LH rats of the same age 3 is not a primary event but results from an extrarenal stimulation of Tx synthetase or of arachidonic acid availability.…”

Section: Figure 4 Graphs Showing Effects Of Thromboxane Ajprostaglanmentioning

confidence: 99%

“…According to previous experiments, 16 baseline parameters were obtained during a 10-minute period after a stabilization period of 30 minutes. Then graded concentrations of NE (Levophed, Winthrop, Clichy, France) were infused (Precidor 5003, Infers A.G., Bale, Switzerland) for 3 minutes at 20-minute intervals via a catheter connected to the main perfusion line in six animals of each strain; PHE (Sigma Chemical Co., St. Louis) was given according to the same protocol to the six remaining rats.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…It was conducted by using isolated perfused kidneys, which we have shown to be a valuable model for the study of renal prostanoids. 16 …”

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confidence: 99%

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Adrenergic stimulation of renal prostanoids in the Lyon hypertensive rat.

et al. 1991

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Young, genetically hypertensive Lyon (LH) rats exhibited an increased renal in vivo turnover of norepinephrine and an elevated urinary excretion of thromboxane B 2 when compared with nonnotensive (LN) and low blood pressure (LL) controls. Therefore, the effects of norepinephrine (1.2 xlO" 8 to 9.6 xlO" 7 M) and of phenylephrine (5xlO~8 to 1.9x10"' M) on renal function and the urinary excretion of prostanoids were assessed in isolated perfused kidneys of 8-week-old LH, LN, and LL rats. In addition, the effects of norepinephrine were assessed before and during thromboxane A 2 /prostaglandin H 2 receptor blockade by AH23848 (4xlO~* M). Before drug infusion, LH kidneys differed from those of LN and LL controls by having an elevated renal vascular resistance and a decreased natriuresis and glomerular filtration rate; the urinary output of prostaglandin E 2 and F^, of 6-ketoprostaglandin F la , and of thromboxane B 2 was similar in the three strains. The constrictor effects of norepinephrine and phenylephrine were significantly increased in LH rat kidneys compared with LL but not with LN controls, and their pressure-natriuresis was markedly reduced. Norepinephrine and phenylephrine induced a 10-to 20-fold dose-dependent increase in the synthesis of the four prostanoids, which was more pronounced in LH than in LN and LL rats for thromboxane B 2 only. AH23848 infusion significantly reduced the vascular effects of norepinephrine and increased the natriuretic response of LH but not of LN and LL rat kidneys. In conclusion, isolated perfused kidneys from LH rats exhibit an increased production of thromboxane A 2 , which enhances the renal effects of norepinephrine and therefore could participate in the development of hypertension of the Lyon model. (Hypertension 1991;17:296-302)

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Section: Discussionmentioning

confidence: 99%

Section: Figure 4 Graphs Showing Effects Of Thromboxane Ajprostaglanmentioning

confidence: 99%

Section: Experimental Protocolsmentioning

confidence: 99%

“…It was conducted by using isolated perfused kidneys, which we have shown to be a valuable model for the study of renal prostanoids. 16 …”

mentioning

confidence: 99%

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Adrenergic stimulation of renal prostanoids in the Lyon hypertensive rat.

et al. 1991

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“…), their right kidney was isolated as previously described (Liu et al, 1990). Briefly, after a midline abdominal incision, the right adrenal artery and small lombar arteries were tied off.…”

Section: Isolated Kidney Preparationmentioning

confidence: 99%

Interactions between nitric oxide and prostanoids in isolated perfused kidneys of the rat

Ziyyat

Zhang

Benzoni

1996

British J Pharmacology

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1 The present study was aimed to assess the interaction between nitric oxide (NO) and thromboxane (Tx) A2-prostaglandin (PG) H2 in single-pass perfused isolated kidneys of the rat.2 Noradrenaline (NA, 63 and 110 nM) dose-dependently elevated the renal vascular resistance (RVR), the glomerular filtration rate (GFR) and the urinary excretion of sodium (UNaV). Infusion of Nw-nitro-Larginine methyl ester (L-NAME, 100 gM), an inhibitor of NO synthesis, enhanced the effects of NA on RVR and on UNaV, but decreased those on GFR. The TxA2-PGH2 (TP) receptor blockade by GR32191B (10 gM) attenuated this potentiating effect of L-NAME.3 When renal perfusion pressure was stepwise increased from 90 to 150 mmHg, L-NAME similarly decreased renal perfusion flow rate and GFR. 4 The venous excretion of TxB2 and 6-keto-PGF,c, was increased by L-NAME in baseline conditions as well as after NA or increasing renal perfusion pressure (RPP). 5 These results suggest that: (1) TxA2 and PGH2 play an important role in the overall effect of the renal prostanoids, (2) NO strongly interacts with the cyclo-oxygenase pathway and reduces the prostanoid synthesis in the kidney, and (3) the pressor effect of L-NAME partly relies upon the vasoconstrictor effect of TxA2 and PGH2.

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Effects of Naftidrofuryl on Isolated Perfused Kidneys of Spontaneously Hypertensive Rats

Zhang¹,

Liu²,

Sacquet³

et al. 1993

Clin Exp Pharma Physio

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1. Experiments were designed to determine the effects of low concentrations (5-500 nmol/L) of naftidrofuryl, a 5-hydroxytryptamine (5-HT) antagonist, on renal functions and prostanoid synthesis responses to noradrenaline (NA) and 5-HT. Isolated kidneys of 8 week old male spontaneously hypertensive rats were perfused at a constant flow rate in a single-pass system. 2. In baseline conditions, naftidrofuryl did not modify the renal vascular resistance and the glomerular filtration rate (GFR), although it elicited a significant but not dose-dependent increase in the venous excretion of 6-keto-prostaglandin (PG) F1 alpha and thromboxane (Tx)B2, the stable end-products of PGI2 and TxA2, respectively. 3. NA increased renal vascular resistance and GFR in a dose-dependent manner and enhanced the venous excretion of 6-keto-PGF1 alpha and TxB2. Naftidrofuryl significantly attenuated the effects of NA on renal vascular resistance, abolished those on GFR and enhanced, at the highest concentration (500 nmol/L) only, those on 6-keto-PGF1 alpha excretion. 4. 5-HT increased renal vascular resistance but not GFR. It did not change the sodium excretion and the release of 6-keto-PGF1 alpha and TxB2. Naftidrofuryl blunted the RVR response to 5-HT without change in the prostanoid release. The inhibitory action of naftidrofuryl was not modified by indomethacin which, by itself, prevented the vasoconstrictor response to 5-HT.(ABSTRACT TRUNCATED AT 250 WORDS)

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Basal prostaglandin synthesis by the isolated perfused rat kidney

Cited by 5 publications

References 23 publications

Adrenergic stimulation of renal prostanoids in the Lyon hypertensive rat.

Adrenergic stimulation of renal prostanoids in the Lyon hypertensive rat.

Interactions between nitric oxide and prostanoids in isolated perfused kidneys of the rat

Effects of Naftidrofuryl on Isolated Perfused Kidneys of Spontaneously Hypertensive Rats

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