Basal microRNA expression patterns in reward circuitry of selectively bred high-responder and low-responder rats vary by brain region and genotype

Hamilton, David E.; Cooke, Christopher L.; Carter, Bradley S.; Akil, Huda; Watson, Stanley J.; Thompson, Robert C.

doi:10.1152/physiolgenomics.00152.2013

Cited by 8 publications

(6 citation statements)

References 47 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The current findings are supported by the results of Thompson and colleagues that demonstrated differences in microRNA expression between animals classified as high or low responders in their processing of reward and anxiety (Hamilton et al, 2014). In addition to the mPFC, microRNA regulation in the nucleus accumbens is important in mediating resilience to stress (Dias et al, 2014).…”

Section: Rj Chen 15supporting

confidence: 82%

MicroRNAs as biomarkers of resilience or vulnerability to stress

et al. 2015

View full text Add to dashboard Cite

Identifying novel biomarkers of resilience or vulnerability to stress could provide valuable information for the prevention and treatment of stress-related psychiatric disorders. To investigate the utility of blood microRNAs as biomarkers of resilience or vulnerability to stress, microRNAs were assessed before and after 7days of chronic social defeat in rats. Additionally, microRNA profiles of two important stress-regulatory brain regions, the medial prefrontal cortex (mPFC) and basolateral amygdala (BLA), were assessed. Rats that displayed vulnerability to subsequent chronic stress exhibited reductions in circulating miR-24-2-5p, miR-27a-3p, miR-30e-5p, miR-3590-3p, miR-362-3p, and miR-532-5p levels. In contrast, rats that became resilient to stress displayed reduced levels of miR-139-5p, miR-28-3p, miR-326-3p, and miR-99b-5p compared to controls. In the mPFC, miR-126a-3p and miR-708-5p levels were higher in vulnerability compared to resilient rats. In the BLA, 77 microRNAs were significantly altered by stress but none were significantly different between resilient and vulnerable animals. These results provide proof-of-principle that assessment of circulating microRNAs is useful in identifying individuals who are vulnerable to the effects of future stress or individuals who have become resilient to the effects of stress. Furthermore, these data suggest that microRNAs in the mPFC but not in the BLA are regulators of resilience/vulnerability to stress.

show abstract

Section: Rj Chen 15supporting

confidence: 82%

MicroRNAs as biomarkers of resilience or vulnerability to stress

et al. 2015

View full text Add to dashboard Cite

show abstract

“… 24 , 25 It has been demonstrated that brain-specific miRNA candidates within the amygdala regulate anxiety and fear-related behaviours in mice. 26 , 27 , 28 However, there is a paucity of information about whether the microbiome could regulate amygdala-dependent fear behaviours or modify the expression of miRNAs in the central nervous system.…”

Section: Introductionmentioning

confidence: 99%

The microbiome regulates amygdala-dependent fear recall

et al. 2017

View full text Add to dashboard Cite

The amygdala is a key brain region that is critically involved in the processing and expression of anxiety and fear-related signals. In parallel, a growing number of preclinical and human studies have implicated the microbiome–gut–brain in regulating anxiety and stress-related responses. However, the role of the microbiome in fear-related behaviours is unclear. To this end we investigated the importance of the host microbiome on amygdala-dependent behavioural readouts using the cued fear conditioning paradigm. We also assessed changes in neuronal transcription and post-transcriptional regulation in the amygdala of naive and stimulated germ-free (GF) mice, using a genome-wide transcriptome profiling approach. Our results reveal that GF mice display reduced freezing during the cued memory retention test. Moreover, we demonstrate that under baseline conditions, GF mice display altered transcriptional profile with a marked increase in immediate-early genes (for example, Fos, Egr2, Fosb, Arc) as well as genes implicated in neural activity, synaptic transmission and nervous system development. We also found a predicted interaction between mRNA and specific microRNAs that are differentially regulated in GF mice. Interestingly, colonized GF mice (ex-GF) were behaviourally comparable to conventionally raised (CON) mice. Together, our data demonstrates a unique transcriptional response in GF animals, likely because of already elevated levels of immediate-early gene expression and the potentially underlying neuronal hyperactivity that in turn primes the amygdala for a different transcriptional response. Thus, we demonstrate for what is to our knowledge the first time that the presence of the host microbiome is crucial for the appropriate behavioural response during amygdala-dependent memory retention.

show abstract

“…Следует отметить, что последовательности предшественников ортологичных микроРНК собаки и лисы в основном идентичны. Мы ожидали увидеть разницу в уровнях экспрессии микроРНК в гиппокампе у двух групп лисиц, отличающихся по стресс-ответу, агрессии и тревожности, так как микроРНК-регуляция затрагивает ряд генов, участвующих в этих реакциях в разных структурах мозга (Hamilton et al, 2014;Hollins, Cairns, 2016). Исследование базального уровня микроРНК у двунаправленно селекционируемых групп крыс (bHR и bLR), отличающихся по подобным же признакам, показало достоверные различия между группами в nucleus accumbens и prelimbic cortex.…”

Section: Discussionunclassified

“…D.E. Hamilton с коллегами предполагают наличие подобных различий в профиле микроРНК также в гиппокампе и миндалине (Hamilton et al, 2014). Однако в нашем исследовании не выявлено значимой разницы в экспрессии не только у микроРНК, ортологи которой, по данным литературы, изменяют экспрессию при разных формах стресса (Hollins, Cairns, 2016) Приложение 2, но и у остальных обнаруженных микроРНК.…”

Section: Discussionunclassified

“…Это относится и к генетически закрепленным различиям в поведении и стресс-ответе. Например, линии крыс, селекционированных на высокую (bHR) и низкую (bLR) активность в новых условиях, у которых различны уровни стресс-реакции, агрессивности и других поведенческих реакций, имеют также различия в паттерне экспрессии микроРНК как в покое, так и после стресса в разных структурах мозга (Hamilton et al, 2014). Есть работы и на других видах, в том числе собаках разных пород, имеющих различные характеристики по толерантности к стрессу (Luo et al, 2016).…”

unclassified

See 1 more Smart Citation

Hippocampal glucocorticoid receptor and microRNA gene expression and serum cortisol concentration in foxes selected for behavior toward humans

et al. 2018

View full text Add to dashboard Cite

In many cases, stress reactivity is one of the important bases of aggressive behavior. It appears as if reduced stress reactivity underlies an abrupt decrease in aggression towards man in domesticated animals. However, the mechanisms of this reduction have yet to be resolved. In this work, we used an experimental domestication model, the silver fox selected for many years for the response to humans to study cortisol stress reactivity in tame and aggressive foxes in response to immobilization in human arms. Additionally, these behavioral fox groups were explored for one of the important mechanisms of glucocorticoid negative feedback, the expression of the glucocorticoid receptor gene (NR3C1) in a portion of the dorsal hippocampus. In recent years, attention has been paid to differences in miRNA expression patterns between animals with different behavior and stress reactivity, as well as to miRNA regulation under stress. The same applies to NR3C1 mRNA as well. That is why we performed a miRNA-seq analysis on a portion of the fox dorsal hippocampus. It has been demonstrated that immobilization in human arms leads to significantly higher stressinduced cortisol levels in aggressive than tame foxes. At the same time, no differences have been found between hippocampal NR3C1 gene expression and the pattern of miRNA expression. Thus, reduced stress reactivity in foxes during selection for the absence of aggressive responses and for the presence of emotionally positive responses to humans does not seem to be associated with important mechanisms of regulation such as alterations in hippocampal NR3C1 gene expression or microRNA-mediated silencing.

show abstract

Basal microRNA expression patterns in reward circuitry of selectively bred high-responder and low-responder rats vary by brain region and genotype

Abstract: Hamilton DE, Cooke CL, Carter BS, Akil H, Watson SJ, Thompson RC. Basal microRNA expression patterns in reward circuitry of selectively bred high-responder and low-responder rats vary by brain region and genotype.

Cited by 8 publications

References 47 publications

MicroRNAs as biomarkers of resilience or vulnerability to stress

MicroRNAs as biomarkers of resilience or vulnerability to stress

The microbiome regulates amygdala-dependent fear recall

Hippocampal glucocorticoid receptor and microRNA gene expression and serum cortisol concentration in foxes selected for behavior toward humans

Contact Info

Product

Resources

About