Basal DNA damage in individual human lymphocytes with age

Singh, Narendra P.; Danner, David B.; Tice, Raymond R.; Pearson, Jay D.; Brant, Larry J.; Morrell, Christopher; Schneider, Edward L.

doi:10.1016/0921-8734(91)90026-8

Cited by 121 publications

(51 citation statements)

References 22 publications

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“…Thus, apoptosis in sperm can be assumed to be a parallel pathway to DNA repair in somatic cells because both serve identical functions (namely, to ensure a zero-error transmission either during cell replication or reproduction). We have shown elsewhere an increased level of DNA damage and decreased rate of its repair with aging in somatic cells (93,94). Therefore, our current findings, that DNA damage increases with age and apoptosis decreases with age in sperm, are in accordance with our above speculation that apoptosis and DNA repair are parallel pathways.…”

Section: Discussionsupporting

confidence: 81%

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Singh

Müller

Berger

2003

Fertility and Sterility

395

223

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Section: Discussionsupporting

confidence: 81%

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Singh

Müller

Berger

2003

Fertility and Sterility

395

223

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“…The current study showed no difference in damage to DNA of leukocytes between age groups both in the case of control and exposed individuals. Previously higher DNA damage was noted in lymphocytes of individuals over 60 years of age by Singh et al 38) . However, among our exposed group, there were no individuals older than 60 years of age and among the control individuals, there was only one woman, who was 66 years old.…”

Section: Discussionmentioning

confidence: 85%

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Dobrzyńska¹,

Pachocki²,

Gajowik³

et al. 2014

Journal of Occupational Health

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The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel: Małgorzata M. DOBRZYŃSKA, et al. Department of Radiation Hygiene and Radiobiology, National Institute of Public Health – National Institute of Hygiene, Poland— Objectives The aim of this study was estimation of DNA strand breaks in leukocytes of peripheral blood of staff in a nuclear medicine department. Methods The exposed group consisted of 46 volunteers and the control group consisted of 40 volunteers. Samples consisting of 1 ml whole blood were collected by venepuncture. DNA damage in leukocytes was detected by alkaline comet assay. Results There was no correlation between the effective dose measured by individual dosimeters and DNA damage and no differences between sexes. The mean level of damage to DNA in people exposed to ionizing radiation was significantly elevated compared with control individuals. The highest value for mean comet tail moment was noted in leukocytes of PET/CT and scintigraphy technicians (1.28 vs. 0.30 for control, p=0.013). The levels of DNA damage in leukocytes of workers in category B (effective dose may exceed 1 mSv/year) were significantly enhanced. The DNA migration of leukocytes in exposed smokers and nonsmokers was similar. In the control group the damage to DNA of leukocytes in smokers was markedly but not significantly higher compared with nonsmokers. Conclusions Occupational exposure to ionizing radiation leads to enhanced levels of reversible DNA damage in leukocytes of nuclear medicine employees. The level of DNA damage depends on the kind of work. Cigarette smoking is related to the increase in DNA damage in unexposed individuals but not in nuclear medicine workers. Radiation seems to be a stronger inducer of DNA damage than smoking. Although most of the DNA damage detected by comet assay is repaired, further improvement of radiation safety should be taken under consideration.

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“…Singh and co-workers (30) observed that although DNA damage significantly differed with age, the mean levels of DNA damage increased only slightly. The study sample generally consisted of young and middle age individuals.…”

Section: Discussionmentioning

confidence: 99%

“…In the study, 60 smokers (30 A small questionnaire for gathering the demographic and ethnic information was also given to the individuals, and the individuals stating themselves as Turkish were included in the study. Each subject filled in detailed questionnaires regarding confounding factors for DNA damage such as smoking.…”

Section: Study Subjectsmentioning

confidence: 99%

Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups

KAYAALTI¹

2012

Ankara Üniversitesi Tıp Fakültesi Mecmuası

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Aim:Smoking poses a serious threat to public health. The aim of this study was to investigate the relationship between smoking and DNA damage in lymphocytes. A potential genotoxic effect of cigarette smoking was analyzed with the nine comet assay parameters including comet length (CL), comet intensity (CI), head length (HL), head intensity (HI), tail length (TL), tail intensity (TI), DNA tail (DNAt), tail moment (TM) and olive tail moment (OTM). For the first time in this study, smokers were grouped as female and male, and nine comet parameters were used.Material and Method: 120 volunteers (60 non-smokers, 60 smokers) were monitored in the way of DNA damage in blood lymphocytes. The levels of DNA damage was measured by BAB Bs Comet Assay system.Results: Highly significant associations were found between the non-smoker and smoker groups for CI, TL and OTM comet parameters (p<0.01). Smoker female group had higher CL, CI, HL, HI, TL, TI (p<0.01) and TM (p<0.05) with regard to DNA damages than the non-smoker female group. In contrast, only DNAt, and OTM comet parameters were statistically significantdifferences between the smoker male and non-smoker male groups (p<0.05). When the smoking index (SI) of all the blood samples from females were compared based on all studied comet parameters, statistically significant association was found except for TM. On the other hand, the blood samples taken from males were statistically significant in terms of CL, HL, HI, TI and OTM parameters (p<0.05). Conclusion:Consequently, it can be said that, smoking cause DNA damages and females are more sensitive to the effect of the smoking than males.

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Basal DNA damage in individual human lymphocytes with age

Cited by 121 publications

References 22 publications

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Effects of age on DNA double-strand breaks and apoptosis in human sperm

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups

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