BARX2 and estrogen receptor-α (ESR1) coordinately regulate the production of alternatively spliced ESR1 isoforms and control breast cancer cell growth and invasion

Stevens, Tracy Alison; Meech, Robyn

doi:10.1038/sj.onc.1209529

Cited by 64 publications

(69 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These genes included cadherins, integrin, laminins and matrix metalloproteinase 2 (Mmp2). The latter result is consistent with our previous findings that Barx2 regulates MMP and tissue inhibitors of metalloproteinases (TIMP) genes (Stevens et al, 2004;Stevens and Meech, 2006). …”

Section: Barx2 Regulates Cell Matrix and Adhesion Molecules During Lgsupporting

confidence: 82%

“…6F). We have reported similar effects of Barx2 overexpression in mammary epithelial cells (Stevens and Meech, 2006). This suggests a role for Barx2 in driving complex epithelial cell organization, probably via regulated cell adhesion and MMP-dependent ECM remodeling.…”

Section: Barx2 Regulates Mmp Expression and Migration Of The Cells Thsupporting

confidence: 57%

“…We found previously that Barx2 regulates cell adhesion and matrix remodeling (Meech et al, 2005;Stevens and Meech, 2006). Cell adhesion and cell-matrix interactions are crucial during branching morphogenesis of LGs and other ocular glands.…”

Section: Barx2 Regulates Cell Matrix and Adhesion Molecules During Lgmentioning

confidence: 99%

“…We and others have shown that the homeodomain transcription factor Barx2, which is related to the Drosophila Bar family genes, is expressed in several epithelial tissues that develop through branching morphogenesis such as lung, kidney, mammary gland, and lacrimal and salivary gland primordia (Herring et al, 2001;Jones et al, 1997;Olson et al, 2005;Sander et al, 2000;Sellar et al, 2001;Sellar et al, 2002;Stevens and Meech, 2006). However, the role of Barx2 during branching morphogenesis has not been determined.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Barx2 and Fgf10 regulate ocular glands branching morphogenesis by controlling extracellular matrix remodeling

et al. 2011

View full text Add to dashboard Cite

SUMMARYThe lacrimal gland (LG) develops through branching morphogenesis and produces secretions, including tears, that lubricate and protect the ocular surface. Despite the prevalence of LG disorders such as dry eye, relatively little is known about the regulation of LG development. In this study, we show that the homeobox transcription factor Barx2 is highly expressed in conjunctival epithelium, eyelids and ocular [lacrimal, harderian (HG), and meibomian (MG)] glands and is necessary for normal ocular gland and eyelid development. Barx2 -/-mice show defective LG morphogenesis, absence of the HG, and defects in MG and eyelid fusion. Ex vivo antisense assays confirm the requirement for Barx2 in LG bud elongation and branching. Gene expression profiles reveal decreased expression of several adhesion and matrix remodeling molecules in Barx2 -/-LGs. In culture, Barx2 regulates expression of matrix metalloproteinases (MMPs) and epithelial cell migration through the extracellular matrix. Fibroblast growth factors are crucial regulators of LG development and we show that Barx2 is required for Fgf10-induced LG bud elongation and that both Barx2 and Fgf10 cooperate in the regulation of MMPs. Together, these data suggest a mechanism for the effects of loss of Barx2 on ocular gland development. Intriguingly, salivary glands that also express a high level of Barx2 develop normally in Barx2 -/-mice and do not show altered levels of MMPs. Thus, the function of Barx2 is specific to ocular gland development. Based on our data, we propose a functional network involving Barx2, Fgf10 and MMPs that plays an essential role in regulating branching morphogenesis of the ocular glands.

show abstract

Section: Barx2 Regulates Cell Matrix and Adhesion Molecules During Lgsupporting

confidence: 82%

Section: Barx2 Regulates Mmp Expression and Migration Of The Cells Thsupporting

confidence: 57%

Section: Barx2 Regulates Cell Matrix and Adhesion Molecules During Lgmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Barx2 and Fgf10 regulate ocular glands branching morphogenesis by controlling extracellular matrix remodeling

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The ESRα gene encodes for multiple mRNAs that are translated into full-length 66kDa ESRα (ESRα-66), or alternatively spliced to produce truncated forms of the [82].…”

Section: Receptor Alternative Splicing U Estrogen Receptormentioning

confidence: 99%

Alternative Splicing in Prostate and Breast Cancer

Watson¹,

Watson²

2010

TOCJ

View full text Add to dashboard Cite

UAb stract: Alternative splicing of mRNA precursors allows the synthesis of multiple mRNAs from a single primary transcript, thus contributing to proteomic diversity in higher eukaryotes. Multiple studies demonstrate that alternative splicing patterns are altered during cancer progression. Several different mechanisms can contribute to changes in the regulation of alternative splicing. This report will provide an overview of how splicing microarrays and large-scale sequencing are being used to identify splicing changes in breast and prostate cancer. Global analyses of splice variants have identified cancer-specific splice variant patterns that have potential use as biomarkers and potentially have prognostic value as well as may represent novel therapeutic targets. A description of specific splice variants with differential expression in these cancers and their possible functions will be presented.

show abstract

Elucidating evolutionarily conserved mechanisms of diapause regulation using an in silico approach

Ajit

Murphy²,

Banerjee

2021

FEBS Letters

View full text Add to dashboard Cite

Embryonic diapause is an enigmatic phenomenon that appears in diverse species. Although regulatory mechanisms have been established, there is much to be discovered. Herein, we have made the first comprehensive attempt to elucidate diapause regulatory mechanisms using a computational approach. We found transcription factors unique to promoters of genes in diapause species. From pathway analysis and STRING PPI networks, the signaling pathways regulated by these unique transcription factors were identified. The pathways were then consolidated into a model to combine various known mechanisms of diapause regulation. This work also highlighted certain transcription factors that may act as ‘master transcription factors’ to regulate the phenomenon. Promoter analysis further suggested evidence for independent evolution for some of regulatory elements involved in diapause.

show abstract

BARX2 and estrogen receptor-α (ESR1) coordinately regulate the production of alternatively spliced ESR1 isoforms and control breast cancer cell growth and invasion

Cited by 64 publications

References 50 publications

Barx2 and Fgf10 regulate ocular glands branching morphogenesis by controlling extracellular matrix remodeling

Barx2 and Fgf10 regulate ocular glands branching morphogenesis by controlling extracellular matrix remodeling

Alternative Splicing in Prostate and Breast Cancer

Elucidating evolutionarily conserved mechanisms of diapause regulation using an in silico approach

Contact Info

Product

Resources

About