Barriers built on claudins

Turksen, Kursad; Troy, T.-C.

doi:10.1242/jcs.117.18.4341

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…In addition to the classic barrier and fence functions of tight junctions, claudin also plays an important role in regulating cell proliferation and differentiation. 13,14 Abnormal claudin protein expression changes the normal structure and function of tight junctions to cause the loss of cell polarity and the diffusion of nutrients and other factors necessary for the survival and growth of tumor cells; these effects play an important role in the metastasis and nutrition supply of tumor cells. 15 Claudin protein expression is different in several types of cancer tissues, and some claudin proteins are downregulated or not expressed in cancer tissues.…”

Section: Discussionmentioning

confidence: 99%

Expression and Clinical Significance of Claudin-7 in Patients With Colorectal Cancer

Wang

et al. 2018

Technol Cancer Res Treat

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Background and Objective:Claudin-7 is a component of tight junctions and plays important roles in maintaining cell polarity and tightly connecting the barriers between cells. Abnormal claudin-7 protein expression is closely related to tumor occurrence, development, and metastasis. This study aimed to investigate the correlation between claudin-7 expression and carcinogenesis or metastasis in colorectal cancer and the clinical significance of these relationships.Materials and Methods:In this study, enzyme linked immunosorbent assays (ELISA) were used to measure the claudin-7 levels in serum from patients with colorectal cancer. Immunohistochemical staining and Western blotting were used to measure claudin-7 expression in colorectal cancer tissues, paracancerous tissues, and metastatic tissues.Results:Serum claudin-7 levels were significantly lower in patients with colorectal cancer than in healthy controls. Immunohistochemistry indicated that claudin-7 expression was lower in colorectal cancer tissues than in normal colorectal tissues, and its expression level was positively correlated with the degree of colorectal cancer tissue differentiation. Claudin-7 expression levels were significantly reduced or undetectable in metastatic tissues compared to primary tumor tissues, but there were no significant differences in claudin-7 expression among different metastatic tissues.Conclusion:We confirmed that claudin-7 downregulation was associated with colorectal cancer and metastasis. Claudin-7 could be a tumor suppressor gene for colorectal cancer and may thus serve an early diagnostic marker and a novel therapeutic target.

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Section: Discussionmentioning

confidence: 99%

Expression and Clinical Significance of Claudin-7 in Patients With Colorectal Cancer

Wang

et al. 2018

Technol Cancer Res Treat

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“…TJs, which consist of a complex of integral membrane and associated cytoplasmic proteins, play major roles in cell-to-cell adhesion, regulation of paracellular transport and maintenance of cell polarity (for a review see [6]). In addition, studies have also suggested a role for TJ proteins in recruiting signaling proteins that regulate such processes as gene transcription, cellular division and specialization [7][8][9]. Given the many and diverse roles of TJs it is likely that the loss of TJ integrity may contribute to cancer development and progression.…”

Section: Introductionmentioning

confidence: 99%

Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines

Cuevas¹,

Winters²,

Todd³

2022

J. Cancer

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Deregulation of tight junction (TJ) proteins and the associated disruption of TJ function has been demonstrated to play a role in the development of endometrial cancer. In the current study, we have shown overexpression of claudin-3 and -4 mRNA (by RT-PCR) and protein (by immunoblotting) in a panel of 9 human endometrial cancer cell lines. To further expand our understanding of the complex role of TJ deregulation in endometrial cancer, we also investigated the expression of 84 TJ and TJ-associated genes (encoding the array of proteins that function within the TJ network from the membrane to nuclear signaling pathways) by microarray analysis. Consistent with the claudin-3 and -4 RT-PCR and immunoblot findings described above, we observed overexpression of the claudin-3 and -4 genes by microarray analysis. Further, we observed overexpression of an additional three genes in 8 of the 9 endometrial cancer cell lines: OCLN (occludin), F11R (JAM-A) and TJP3 (ZO-3). OCLN and F11R encode integral membrane proteins whereas TJP3 encodes a cytosolic scaffolding protein that indirectly links membrane TJ proteins to the actin cytoskeleton and cell signaling pathways. Our data suggest that the structural disruption of TJs coupled with the downstream deregulation of signaling pathways involved in cellular proliferation and migration may contribute to the development of endometrial cancer.

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