Barrier Membrane, a Device for Regeneration: Properties and Applications

Abstract

Context: This article reviews the available evidence about the barrier membranes utilized in Guided Tissue Regeneration process to prevent the migration of unfavorable cells to the wound area. Evidence Acquisition: Available evidence about membranes properties and their different uses were reviewed, and the results of clinical and animal studies and systematic reviews were gathered. Results: A large number of existing membranes with different features and compositions may lead to different study results; none of the available membranes can result in %100 predictable outcomes. Conclusions: Effectiveness of membranes in treating intrabony defects is very controversial; however, treating furcation defects using membranes was reported to be successful in a large number of studies.

In vitro Cell Proliferation Assay of Demineralized Dentin Material Membrane in Osteoblastic MC3T3-E1 Cells

“…Bovine dentine is easy to obtain, morphologically similar to human dentine, and possesses protein derivatives to support bone growth, namely BMP and TGF-β. 1,5,9,10 Performance measurement of mitochondrial dehydrogenase, also known as 3-(4,5-dimethyl-2-thiazolyl test) −2,5-diphenyl-2H-tetrazolium bromide (methyl thiazolyl tetrazolium; MTT), is a rapid assessment of cell proliferation and a colorimetric cytotoxicity test that can be lead into the cell metabolism or cell function measurement. The 446 basic idea is that the tetrazole ring would be cut by mitochondrial dehydrogenase in the cytochrome b and c sites of live cells.…”

Recent progress in materials development and biological properties of GTR membranes for periodontal regeneration

“…Numerous researches have combined bioceramics like bioactive glasses (BG), hydroxyapatite (HA), and calcium phosphate (CP) in dental field because of its important biological characteristics like osteoinductivity and osteoconductivity and capability to minimize the natural inorganic bone constituent (Heinemann et al, 2010;Hu et al, 2016;Li et al, 2008). For bone healing/reparation uses and dental membranes, beta tri-calcium phosphate (β-TCP) has been used as an outstanding bioceramic in several medical places because of its excellent osteo-conductivity, bioactivity, and extreme in vivo resorb ability that are beneficial for effective regeneration process (Rad et al, 2017;Soheilifar et al, 2014;Yang et al, 2015). Numerous forms of the membranes including fibers, gels, foams, and mats, synthesized by a range of procedures involving electrospinning, particulate leaching, freeze-drying, selfassembly, solvent casting, and molding have been utilized (Hurt et al, 2014;Rad et al, 2017;Saleem et al, 2011).…”

The influence of collagen membranes and tetracycline on the PDGF-BB expression and osteoblast amount in bone defects healing: Experimental study in mice

“…1,2 The resorbable membrane should facilitate the attachment, proliferation, and migration of cells on the membrane surface and cover the defects from any oral bacteria. 3 The connective tissue cells attachment to the inner surface of the membrane stimulates periodontal regeneration and attachment, helping to stabilize blood clots and membrane integration into the tissues. 3,4 Collagen is selected as one of the materials manufacturing the resorbable membranes.…”

“…3 The connective tissue cells attachment to the inner surface of the membrane stimulates periodontal regeneration and attachment, helping to stabilize blood clots and membrane integration into the tissues. 3,4 Collagen is selected as one of the materials manufacturing the resorbable membranes. 4 Collagen also has biological activities that play an important role in the formation of coagulum, chemotaxis, and activating the neutrophils and fibroblast cells of periodontal ligaments and gums, with low immunogenicity properties.…”