The nascent state of the nanoproduct industry calls for
important early assessment of environmental impacts before
significant releases have occurred. Clearly, the impact
of manufactured nanomaterials on key soil processes must
be addressed so that an unbiased discussion concerning
the environmental consequences of nanotechnology
can take place. In this study, soils were treated with either
1 μg C60 g-1 soil in aqueous suspension (nC60) or 1000
μg C60 g-1 soil in granular form, a control containing equivalent
tetrahydrofuran residues as generated during nC60
formation process or water and incubated for up to 180
days. Treatment effects on soil respiration, both basal and
glucose-induced, were evaluated. The effects on the soil
microbial community size was evaluated using total
phospholipid derived phosphate. The impact on community
structure was evaluated using both fatty acid profiles
and following extraction of total genomic DNA, by DGGE
after PCR amplification of total genomic DNA using bacterial
variable V3 region targeted primers. In addition, treatment
affects on soil enzymatic activities for β-glucosidase, acid-phosphatase, dehydrogenase, and urease were followed.
Our observations show that the introduction of fullerene, as
either C60 or nC60, has little impact on the structure and
function of the soil microbial community and microbial
processes.