Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei

Abstract

Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a 'Lenetah' × 'Grannelose Zweizeilige' (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a '95SR316A' × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.

Genome Wide Association Mapping of Seedling and Adult Plant Resistance to Barley Stripe Rust (Puccinia striiformis f. sp. hordei) in India

“…Out of the 45 QTL identified for resistance at SRT, 15 were coincident with prior reports Those QTL were reported from different barley germplasm and different Psh races (Thomas et al, 1995 ; Toojinda et al, 2000 ; Vales et al, 2005 ; Rao et al, 2007 ; Verhoeven et al, 2011 ; Gutiérrez et al, 2015 ; Dracatos et al, 2016 ; Esvelt Klos et al, 2016 ; Belcher et al, 2018 ). For Psh resistance at the APS stage using different germplasm and races, only 3 out of the 18 QTL detected, shown in Table 5 , are coincident with previous reports (Vales et al, 2005 ; Rao et al, 2007 ; Verhoeven et al, 2011 ; Gutiérrez et al, 2015 ; Dracatos et al, 2016 ; Belcher et al, 2018 ).…”

Identification of QTL conferring resistance to stripe rust (Puccinia striiformis f. sp. hordei) and leaf rust (Puccinia hordei) in barley using nested association mapping (NAM)

“…Depending on environmental conditions, there is a high variability in disease severity between years. While Psh in general has been of minor economic importance over the last several decades the importance of Ph has increased [ 4 , 5 , 9 – 11 ]. Nevertheless Psh remains a major economic threat, especially for barley production in Australia where it is not yet present, as studies in Mexico identified that 70% of Australian barley varieties are susceptible to the aggressive Psh race 24 [ 12 , 13 ].…”

“…Over the last several decades less research has been conducted on resistance of barley to Psh due to its significantly lower importance compared to Ph . However, 26 uniquely different Rps ( Resistance to Puccinia striiformis ) major genes (reviewed in [ 40 ]) and several QTL [ 11 , 38 , 41 – 53 ] have been reported up to now.…”

Seedling and adult‐plant stage resistance of a world collection of barley genotypes to stripe rust

“…() and Esvelt Klos et al. () reported QTL mapping of PSH resistance at seedling stage using European and North American PSH races, respectively. Often, all‐stage resistance genes are dominant in nature with large effects.…”