Barley plants over-expressing the NAC transcription factor gene<i>HvNAC005</i>show stunting and delay in development combined with early senescence

Christiansen, Michael W.; Matthewman, Colette; Podzimska-Sroka, Dagmara; O'Shea, Charlotte; Lindemose, Søren; Møllegaard, Niels Erik; Holme, Inger Bæksted; Hebelstrup, Kim H.; Skriver, Karen; Gregersen, Per L.

doi:10.1093/jxb/erw286

Cited by 36 publications

(27 citation statements)

References 59 publications

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“…By contrast, the C‐terminal region of NAC proteins is variable, consistent with their diverse transcriptional activation activities (Olsen et al ). NAC TFs are involved in various biological processes, including development, metabolism of reactive oxygen species, senescence, secondary cell wall biosynthesis and cell death (Shang et al ; Nakano et al ; Zhao et al ; Baranwal and Khurana ; Christiansen et al ; Lee et al ).…”

Section: Introductionmentioning

confidence: 99%

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust

Wang

Wei

Song

et al. 2018

JIPB

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NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30, from a cDNA library constructed from wheat (Triticum aestivum) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one-hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C-terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst. Silencing of TaNAC30 by virus-induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H O . Finally, we overexpressed TaNAC30 in fission yeast and determined that cell viability was severely reduced in TaNAC30-transformed cells grown on medium containing H O . These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat-Pst interaction.

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Section: Introductionmentioning

confidence: 99%

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust

Wang

Wei

Song

et al. 2018

JIPB

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“…9, G-J). Since these transcription factors or their close orthologs have been identified as leaf senescence regulators (Distelfeld et al, 2014;Christiansen et al, 2016;Mao et al, 2017), these observations emphasize that several transcriptional regulators, previously associated with leaf senescence, showed an agedependent increase in expression also in roots. Moreover, according to the barley transcriptome atlas (https://webblast.ipk-gatersleben.de/barley_ibsc/), HvWRKY53, HvWRKY70-like, and two AP2-type transcription factors were upregulated in old versus young roots but not expressed in leaves ( Supplemental Table S12).…”

Section: Plant-age-and Tissue-age-dependent Transcriptome Analysis Ofmentioning

confidence: 62%

“…Enhanced expression of genes like HvNAC005, HvNAC003, HvNAM1, and HvNAM2 (Fig. 9), themselves or their close orthologs being proven transcriptional regulators of leaf senescence (Uauy et al, 2006;Distelfeld et al, 2014;Christiansen et al, 2016), implies their involvement in transcriptional control of senescence processes also in roots. In addition, roots expressed a number of NAC-, WRKY-, and AP2-type transcription factors, such as HvWRKY53 that show no presence in young or senescing leaves ( Supplemental Table S12).…”

Section: Distinct and Common Features Of Root Versus Leaf Senescencementioning

confidence: 99%

“…Such a temporal cascade is highly reminiscent of the NAC-ABA-SAG regulatory module that is induced by OsNAC2 in senescing rice leaves (Mao et al, 2017). Overexpression of the transcription factor OsNAC2, a homolog of HvNAC003, accelerates the onset of leaf senescence by binding to promoters of genes involved in chlorophyll degradation and in ABA biosynthesis (Christiansen et al, 2016;Mao et al, 2017). Hormonal signaling by ABA may additionally be involved in ceasing root elongation and root activity, and subsequently, in the decrease of auxin and CK levels (Figs.…”

Section: A Chronological Sequence Of Senescence Processes In Barley Smentioning

confidence: 99%

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An Age-Dependent Sequence of Physiological Processes Defines Developmental Root Senescence

et al. 2019

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Aging-related processes in plant tissues are associated with changes in developmental and physiological processes relevant for stress tolerance and plant performance. While senescence-regulated processes have been extensively characterized in leaves, they remain poorly described in roots. Here, we investigated the physiological processes and molecular determinants underlying the senescence of seminal roots in hydroponically grown barley (Hordeum vulgare). Transcriptome profiling in apical and basal root tissues revealed that several NAC-, WRKY-, and APETALA2 (AP2)-type transcription factors were upregulated just before the arrest of root elongation, when root cortical cell lysis and nitrate uptake, as well as cytokinin concentrations ceased. At this time point, root abscisic acid levels peaked, suggesting that abscisic acid is involved in root aging-related processes characterized by expression changes of genes involved in oxidative stress responses. This temporal sequence of aging-related processes in roots is highly reminiscent of typical organ senescence, with the exception of evidence for the retranslocation of nutrients from roots. Supported by the identification of senescence-related transcription factors, some of which are not expressed in leaves, our study indicates that roots undergo an intrinsic genetically determined senescence program, predominantly influenced by plant age.

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“…Full length Nucleus Inhibits the GA 3 up regulation of αamylase expression in aleurone [64] 29 HvNAC005 (Hordeum vulgare) Full length and C-terminal -Positively regulates senescence [133] 30 HvNAC6 (Hordeum vulgare) Full length Nucleus Positively regulates penetration resistance [134] 31 JUB1/JUNGBRUNNEN1 (Arabidopsis thaliana)…”

Section: C-terminal Nucleusmentioning

confidence: 99%

May the Fittest Protein Evolve: Favoring the Plant‐Specific Origin and Expansion of NAC Transcription Factors

Mathew

Agarwal

2018

BioEssays

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Plant-specific NAC transcription factors (TFs) evolve during the transition from aquatic to terrestrial plant life and are amplified to become one of the biggest TF families. This is because they regulate genes involved in water conductance and cell support. They also control flower and fruit formation. The review presented here focuses on various properties, regulatory intricacies, and developmental roles of NAC family members. Processes controlled by NACs depend majorly on their transcriptional properties. NACs can function as both activators and/or repressors. Additionally, their homo/hetero dimerization abilities can also affect DNA binding and activation properties. The active protein levels are dependent on the regulatory cascades. Because NACs regulate both development and stress responses in plants, in-depth knowledge about them has the potential to help guide future crop improvement studies.

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Barley plants over-expressing the NAC transcription factor geneHvNAC005show stunting and delay in development combined with early senescence

Abstract: HighlightHvNAC005 was shown to be a strong positive regulator of senescence, involved in regulation in the cross field of different hormone and signalling pathways controlling developmental senescence in barley.

Cited by 36 publications

References 59 publications

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust

An Age-Dependent Sequence of Physiological Processes Defines Developmental Root Senescence

May the Fittest Protein Evolve: Favoring the Plant‐Specific Origin and Expansion of NAC Transcription Factors

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