Barcoding of Cryptic Stages of Marine Brown Algae Isolated from Incubated Substratum Reveals High Diversity in Acinetosporaceae (Ectocarpales, Phaeophyceae)1

New records and observations of macroalgae and associated pathogens from the Falkland Islands, Patagonia and Tierra del Fuego

“…Techniques such as the germling emergence method (Peters et al 2015), combined with molecular identification, offer great potential for unraveling more of the algal diversity of little-studied regions such as these. Also, the lack of observations of reproductive structures in many field surveys demonstrates the importance of laboratory culture studies for the resolution of taxonomic and biogeographic problems.…”

“…Uni-algal laboratory cultures from such samples were established following the modified technique described by Müller and Ramírez (1994) and Peters et al (2015).…”

Two species of the genus Acinetospora (Ectocarpales, Phaeophyceae) from Japan: A. filamentosa comb. nov. and A. asiatica sp. nov.

“…The morphological differences we observed were also supported by our molecular data since these two species showed differences from European A. crinita of 3.0-4.7% in rbcL and of 11.3-15.8% in cox1. These sequence differences are large enough for distinguishing ectocarpacean species (Siemer et al 1998, Peters et al 2015. Further, the Greek samples of A. crinita used in cox1 analyses were observed to produce monospores (Peters, pers.com.…”

“…The cox1 sequences produced were aligned with published sequences of the Acinetospora cluster (Peters et al 2015), which included a European A. crinita. Pairwise sequence differences (p-distances) were calculated in MEGA5.2 (Tamura et al 2011).…”

<div class="page" title="Page 1"><div class="layoutArea"><div class="column">Reinstatement of Phrix (Delesseriaceae, Rhodophyta) based on DNA sequence analyses and morpho-anatomical evidence</div></div></div>

“…Easter Island lies in the Chilean Marine National Park Nazca-Desventuradas. The substratum sample was sent to AFP for incubation and isolation of emerging macroalgae following Peters et al (2015). A minute red algal blade developing on a small pebble was isolated and served to generate a unialgal culture (code IP14-38), which was used for all further studies.…”