Barcoded Microchips for Biomolecular Assays

Zhang, Yi; Sun, Jiashu; Zou, Yu; Chen, Wenwen; Zhang, Wei; Xi, Jianzhong; Jiang, Xingyu

doi:10.1021/ac5032379

Cited by 35 publications

(26 citation statements)

References 35 publications

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“…However, the barcode-based assay can also be applied for the simultaneous detection of pathogen-specific oligonucleotides by a single chip containing both positive and negative controls. 155 …”

Section: Laboratory-based Diagnosis (“High-throughput Screening”)mentioning

confidence: 99%

Diverse Applications of Nanomedicine

Pelaz¹,

et al. 2017

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The design and use of materials in the nanoscale size range for addressing medical and health-related issues continues to receive increasing interest. Research in nanomedicine spans a multitude of areas, including drug delivery, vaccine development, antibacterial, diagnosis and imaging tools, wearable devices, implants, high-throughput screening platforms, etc. using biological, nonbiological, biomimetic, or hybrid materials. Many of these developments are starting to be translated into viable clinical products. Here, we provide an overview of recent developments in nanomedicine and highlight the current challenges and upcoming opportunities for the field and translation to the clinic.

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Section: Laboratory-based Diagnosis (“High-throughput Screening”)mentioning

confidence: 99%

Diverse Applications of Nanomedicine

Pelaz¹,

et al. 2017

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“…In addition to PSA, we included DNA analogs of the microRNA sequences miR-30c and miR-141 based on literature demonstrating the utility of microRNA biomarkers in blood for detection of prostate cancer. [28,29] To detect this panel, we designed three nanoswitches with different loop sizes and created a multiplexed nanoswitch mixture and tested detection of all biomarkers in buffer (Figure 5c). We then spiked the biomarkers in 20% serum and showed detection of individual biomarkers (Figure 5c, lanes 2-4) In this study, we showed that DNA nanoswitch barcodes can be used to detect up to six biomarkers in a single assay, and even a mixture of protein, antibody and nucleic acids.…”

Section: Such a System Can Gather Information From Multiple Types Of mentioning

confidence: 99%

DNA nanoswitch barcodes for multiplexed biomarker profiling

Chandrasekaran

MacIsaac

Vilcapoma

et al. 2020

Preprint

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The detection of molecular biomarkers plays a key role in the clinic, aiding in diagnostics and prognostics, and in the research laboratory, contributing to our basic understanding of diseases. The ability to detect multiple and diverse molecular biomarkers within a single accessible assay would have great utility, providing a more comprehensive picture for clinical evaluation and research, but is a challenge with standard methods. One promising approach is the use of dynamic DNA nanostructures that can respond to molecular biomarkers, which have recently been used in a variety of biosensing strategies. In this work, we report the use of programmable DNA nanoswitches for the multiplexed detection of up to 6 biomarkers within a single pot through the use of a barcoded gel-based readout. We demonstrate the barcoding capability using gene fragments that correspond to 6 different diseases, with each fragment or combination of fragments producing a unique barcode signature. As a defining feature of our method, we show "mixed multiplexing" for simultaneous barcoded detection of different types of biomolecules - DNA, RNA, antibody and protein in a single assay. To demonstrate clinical potential, we show multiplexed detection of a prostate cancer biomarker panel in serum that includes two microRNA sequences and prostate specific antigen (PSA). This strategy holds promise in clinical diagnostics for profiling complex and diverse biomarker panels.

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“…To realize accurate and multiplexed biochemical assays in an even better way, we develop a barcoded microchips for biomolecular assays according to barcode symbology specification (Figure 2c). [21] Using the barcode reader/smartphone, up to 20 samples can be detected simultaneous in a single barcoded chip.…”

Section: Reviewmentioning

confidence: 99%

Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

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115

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Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user‐friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials‐related aspects of the recent advances in microfluidics‐based immunoassays for point‐of‐care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

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Barcoded Microchips for Biomolecular Assays

Cited by 35 publications

References 35 publications

Diverse Applications of Nanomedicine

Diverse Applications of Nanomedicine

DNA nanoswitch barcodes for multiplexed biomarker profiling

Materials for Microfluidic Immunoassays: A Review

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