Barbellatanic acid, a new antitrypanosomal pseudo-disesquiterpenoid isolated from Nectandra barbellata, displayed interaction with protozoan cell membrane

Londero, Vinicius S.; Rosa, Matheus Elias; Baitello, João Batista; Costa-Silva, Thaís A.; Cruz, Lucas Monteiro Santa; Tempone, André G.; Caseli, Luciano; Lago, João Henrique G.

doi:10.1016/j.bbamem.2023.184184

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…The maximum permeabilization was obtained in the presence of 0.5% ( v / v ) of Triton X-100, and untreated parasites were used as the negative control (100% viability, integral membrane). Digital images of the trypomastigotes after treatment with the FII fraction from the M. zeaxanthinifaciens were obtained with a digital fluorescence microscope (EVOS M 5000, Thermo, USA) using the SYTOX Green [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential

Santos Ferreira,

de Castro Levatti,

Santa Cruz

et al. 2024

Pharmaceuticals

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Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals, Tubastraea coccinea, Mussismilia hispida, Madracis decactis, and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera—Vibrio, Shewanella, Mesoflavibacter, Halomonas, Bacillus, and Alteromonas. To conduct this study, EtOAc extracts were prepared and tested against Trypanosoma cruzi. The crude extracts showed IC50 values ranging from 15 to 51 μg/mL against the trypomastigotes. The bacterium Mesoflavibacter zeaxanthinifaciens was selected for fractionation, resulting in an active fraction (FII) with IC50 values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against T. cruzi.

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Section: Methodsmentioning

confidence: 99%

Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential

Santos Ferreira,

de Castro Levatti,

Santa Cruz

et al. 2024

Pharmaceuticals

Self Cite

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show abstract

“…The maximum permeabilization was obtained in the presence of 0.5% (v/v) Triton X-100, and untreated parasites were used as negative control (100% viability, integral membrane) [48]. Digital Images of the trypomastigotes after treatment with FII fraction from Mesoflavibacter zeaxanthinifaciens were obtained in a digital fluorescence microscope (EVOS M 5000, Thermo, USA) using SYTOX Green [49].…”

Section: Evaluation Of Plasma Membrane Permeabilitymentioning

confidence: 99%

Unsaturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Antitrypanosomal Potential

Santos Ferreira,

De Castro Levatti,

Costa

et al. 2024

Preprint

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Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new antitrypanosomal compounds, we investigated the potential of metabolites from bacteria living in corals and sediments of the Southeastern Brazilian coast. Three corals Tubastraea coccinea, Mussismilia hispida, Madracis decactis and sediments yielded 11 bacterial strains, that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera - Vibrio, Shewanella, Mesoflavibacter, Halomonas, Bacillus and Alteromonas. To conduct this study, EtOAc extracts were prepared and tested against Trypanosoma cruzi. Crude extracts showed IC50 values ranging from 15 to 51 μg/mL against trypomastigotes. The bacterium Mesoflavibacter zeaxanthinifaciens was selected for fractionation, resulting in an active fraction (FII) with IC50 values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using NMR and ESI-HRMS analysis, FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate these unsaturated iso-type fatty acids as possible new hits against T. cruzi.

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Exploring the physicochemical properties of the integration of Tristearoyl uridine in Langmuir monolayers: An approach to cell membrane modeling for prodrugs

Moreira,

Escobar,

Giordani

et al. 2024

Biophysical Chemistry

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Barbellatanic acid, a new antitrypanosomal pseudo-disesquiterpenoid isolated from Nectandra barbellata, displayed interaction with protozoan cell membrane

Cited by 3 publications

References 40 publications

Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential

Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential

Unsaturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Antitrypanosomal Potential

Exploring the physicochemical properties of the integration of Tristearoyl uridine in Langmuir monolayers: An approach to cell membrane modeling for prodrugs

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